In vitro antibacterial activity of the volatile oil of Nigella sativa seeds against multiple drug‐resistant isolates of Shigella spp. and isolates of Vibrio cholerae and Escherichia coli

Abstract

The antibacterial activity of the volatile oil of Nigella sufiua seeds was studied against 37 isolates of Shigella dysenteriue I , Shigella &men, ShigeUa sonnei and ShigeUa boydu and 10 strains of Vibrio cholerae and Escherichia coli. Most of the strains were clinically resistant to ampicillin, co-trimoxazole and tetracycline. All the strains tested showed promising sensitivity to the volatile oil. The minimum inhibitory concentration (MIC) of the volatile oil for Shigellu, Vibrio and Escherichia strains tested was between 50-400 pglmL.

Comparison of Chemical Composition and Antibacterial Activity of Nigella sativa Seed Essential Oils Obtained by Different Extraction Methods

“…Previously, different extraction methods, e.g., Clevenger hydrodistillation and steam distillation of seeds or their oils, have been used to obtain antibacterial active essential oil from N. sativa (2,3,10,15,19,23,(30)(31)(32)39). However, according to our results, the techniques based on steam distillation of extracted seed oils (SE-SD and SE-SFE) provide markedly more effective essential oil than do HD and SD of seeds.…”

“…It was found that the antibacterial activity of the seed was predominantly related to the volatile oil (19,30). Subsequently, the antibacterial properties of the volatile oil of N. sativa have been further examined with different types of in vitro (3,10,15,23,32) or in vivo tests (7,14).…”

Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella sativa Linn. Against Breast Cancer Cells

“…5 The seeds are also used as condiments, carminatives, appetizers, stimulants for menstrual flow (emmenagogue), tonics, and for increasing milk yield (galactagogue). 6 A number of studies have validated the traditional uses of N. sativa, such as antibacterial, 7 antihistamine, 8 and antioxidant effects. 9 N. sativa seed extracts and the isolated active principles, thymoquinone (TQ) and dithymoquinone showed cytotoxic activity against several tumor types and prevented tumor growth in mice [10][11][12][13] and induced telomere attrition and apoptosis.…”

Extraction of Essential Oil from Nigella sativa Using Supercritical Carbon Dioxide: Study of Antibacterial Activity

“…It should be noted that the name of black cumin is sometimes given to entirely unrelated spice Nigella sativa. The crude extracts of N. Sativa were reported to have a promising effect on multi-drug resistant S. aureus and Candida albicans [10] , Shigella spp., Vibrio cholerae [15] . Production of β lactamases is the most common mechanism of resistance among the Gram-negative.…”