<i>In vitro</i>anti-influenza activity of a protein-enriched fraction from larvae of the housefly (<i>Musca domestica</i>)

Wang, Furong; Ai, Hui; Lei, Chaoliang

doi:10.3109/13880209.2012.723724

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…Given the high forage and food value of Musca domestica, the cultivation of their larvae and pupae can alleviate the protein feed shortage (Akpodiete et al, 1997;Hwangbo et al, 2009;Iniguez-covarrubias et al, 1994;Miller et al, 1974;Ocio and Vinaras, 1979;van Huis, 2013;Yan et al, 2012;Zuidhof et al, 2003). Additionally, M. domestica larvae, or maggots, contain several biologically active substances, such as antimicrobial peptides, lectin and chitin Fu et al, 2009;Hou et al, 2007;Nayduch and Joyner, 2013;Tang et al, 2009;Wang et al, 2013;Zhang et al, 2013). The supplemental use of maggots in animal feed may improve animals' immune systems and reduce antibiotic usage Dong et al, 2013).…”

Section: Introductionmentioning

confidence: 97%

Housefly maggot-treated composting as sustainable option for pig manure management

et al. 2015

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Section: Introductionmentioning

confidence: 97%

Housefly maggot-treated composting as sustainable option for pig manure management

et al. 2015

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“…Previous studies reported the protein-enriched fraction (PEF) that isolated from the larvae of housefly had strong antiviral activity against influenza virus at a very early stage of the interaction with virus particles or their entry into the cells [ 29 ]. The extract of Ginkgo biloba (EGB) could directly interact with influenza virus and markedly reduce the infectivity of the virus by preventing the adsorption to host cells [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Natural Influenza A H1N1 Virus Neuraminidase Inhibitory Peptide Derived from Cod Skin Hydrolysates and Its Antiviral Mechanism

Yuan

Zeng

et al. 2018

Marine Drugs

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In this paper, a novel natural influenza A H1N1 virus neuraminidase (NA) inhibitory peptide derived from cod skin hydrolysates was purified and its antiviral mechanism was explored. From the hydrolysates, novel efficient NA-inhibitory peptides were purified by a sequential approach utilizing an ultrafiltration membrane (5000 Da), sephadex G-15 gel column and reverse-phase high-performance liquid chromatography (RP-HPLC). The amino acid sequence of the pure peptide was determined by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) was PGEKGPSGEAGTAGPPGTPGPQGL, with a molecular weight of 2163 Da. The analysis of the Lineweacer–Burk model indicated that the peptide was a competitive NA inhibitor with Ki of 0.29 mM and could directly bind free enzymes. In addition, docking studies suggested that hydrogen binding might be the driving force for the binding affinity of PGEKGPSGEAGTAGPPGTPGPQGL to NA. The cytopathic effect reduction assay showed that the peptide PGEKGPSGEAGTAGPPGTPGPQGL protected Madin–Darby canine kidney (MDCK) cells from viral infection and reduced the viral production in a dose-dependent manner. The EC50 value was 471 ± 12 μg/mL against H1N1. Time-course analysis showed that PGEKGPSGEAGTAGPPGTPGPQGL inhibited influenza virus in the early stage of the infectious cycle. The virus titers assay indicated that the NA-inhibitory peptide PGEKGPSGEAGTAGPPGTPGPQGL could directly affect the virus toxicity and adsorption by host cells, further proving that the peptide had an anti-viral effect with multiple target sites. The activity of NA-inhibitory peptide was almost inactivated during the simulated in vitro gastrointestinal digestion, suggesting that oral administration is not recommended. The peptide PGEKGPSGEAGTAGPPGTPGPQGL acts as a neuraminidase blocker to inhibit influenza A virus in MDCK cells. Thus, the peptide PGEKGPSGEAGTAGPPGTPGPQGL has potential utility in the treatment of the influenza virus infection.

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“…For example, typical strategies of TCM treatment in hepatitis B are (1) to decrease the load of HBV viral; (2) to improve liver function; (3) to ameliorate liver inflammation; (4) to improve immune function; (5) to regulate lipid metabolism; and (6) to ameliorate hepatic fibrosis [21, 22]. The protein-enriched fraction extract from traditional Chinese medicines, housefly larvae, has been found to possess potential hepatoprotective effect [13], immunomodulatory function [12], inflammation regulation effect [24], and antiviral activity against pseudorabies virus [14] and influenza virus [7]. However, the anti-HBV active of the protein-enriched extracts of housefly larvae, until now, has not been studied.…”

Section: Discussionmentioning

confidence: 99%

“…Housefly ( Musca domestica ) can thrive without causing infection in the most unfavorable surroundings, largely due to its antimicrobial and immunoactive substances in the immune system [7]. Musca domestica larvae are traditionally used as therapeutic agents for decubital necrosis, malnutritional stagnation, and ecthyma since the Ming/Qing Dynasty (1368 Anno Domini) up to now in China [8].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the effects of antioxidant [9], antibacterial [10], antitumor activities [11], and immunomodulatory functions [12] of the protein-enriched extracts of housefly larvae have been reported. Additionally, the protein-enriched extracts of housefly larvae have been reported to possess potential hepatoprotective effect [13] and antiviral activity against pseudorabies virus [14] and influenza virus [7]. However, it is unclear whether the protein-enriched extracts of housefly larvae have potent antiviral activity against hepatitis B virus.…”

Section: Introductionmentioning

confidence: 99%

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Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line

Jin

Wang

et al. 2014

The Scientific World Journal

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Hepatitis B virus (HBV) infection remains a major public health problem. Although several vaccines and therapeutic strategies are currently being implemented to combat HBV virus, effective antiviral therapy against HBV infection has not been fully developed. Alternative strategies and new drugs to combat this disease are urged. Insects and insect derivatives are a large and unexploited source of potentially useful compounds for modern medicine. In the present study, we investigated the first anti-HBV activity of a protein-enriched fraction (PE) from the larvae of the housefly (Musca domestica) in a stable HBV-producing cell line. HBsAg and HBeAg in the culture medium were measured by enzyme-linked immunosorbent assay. HBV-DNA was quantified by fluorescent quantification PCR. HBV core protein was assayed by immunofluorescent staining. Results indicate PE treatment inhibited both HBsAg, HBeAg secretion, and HBV-DNA replication. Furthermore, PE could also suppress HBV core protein expression. PE could be a potential candidate for the development of a novel and effective drug for the treatment of HBV infection.

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In vitroanti-influenza activity of a protein-enriched fraction from larvae of the housefly (Musca domestica)

Cited by 12 publications

References 28 publications

Housefly maggot-treated composting as sustainable option for pig manure management

Housefly maggot-treated composting as sustainable option for pig manure management

A Novel Natural Influenza A H1N1 Virus Neuraminidase Inhibitory Peptide Derived from Cod Skin Hydrolysates and Its Antiviral Mechanism

Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line

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