In vitroanti-inflammatory activity of spherical silver nanoparticles and monodisperse hexagonal gold nanoparticles by fruit extract ofPrunus serrulata: a green synthetic approach

Abstract

Recently, green metal nanoparticles have received global attention owing to their economical synthesis, biocompatible nature, widespread biomedical and environmental applications. Current study demonstrates a sustainable approach for the green synthesis of silver nanoparticles (P-AgNPs) and gold nanoparticles (P-AuNPs) from P. serrulata fresh fruit extract. The silver and gold nanoparticles were synthesized in a very rapid, efficient and facile manner, within 50 min and 30 s at 80 °C, respectively. The nanoparticles were characterized by using visual observation, UV-Vis, FE-TEM, EDX, elemental mapping, FT-IR, XRD and DLS, which confirmed the formation of monodispersed, crystalline and stable nanoparticles. Further, we explored these nanoparticles for anti-inflammatory activity through inhibition of downstream NF-κB activation in macrophages (RAW264.7). We demonstrated that the nanoparticles reduced expression of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PEG), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was attenuated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Furthermore, nanoparticles significantly suppressed LPS-induced activation of NF-κB signalling pathway via p38 MAPK in RAW 264.7 cells. To the best of our knowledge, this is the first report on the efficient green synthesis of P-AgNPs and P-AuNPs using P. serrulata fresh fruit extract and its in vitro anti-inflammatory effects. Collectively, our results suggest that P. serrulata fresh fruit extract is a green resource for the eco-friendly synthesis of P-AgNPs and P-AuNPs, which further can be utilized as a novel therapeutic agent for prevention and cure of inflammation due to their biocompatible nature.

<p>The Antibacterial Mechanism of Silver Nanoparticles and Its Application in Dentistry</p>

“…44 Silver nanoparticles were reported to possess an antiinflammatory effect, through modulation of the level of inflammatory cytokines and growth factors. 45 Periodontal dressing coated with silver nanoparticles can be used to treat gingival wounds. This treatment accelerates the early phases of wound healing, with apparently new collagen synthesis and neovascularization.…”

Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiola rosea rhizome extracts

“…The crystallinity of the biosynthesized nanoparticles was evaluated by selected area (electron) diffraction (SAED) [22,33]. The multiple electron diffraction patterns corresponding to the polycrystalline nature of the synthesized nanoparticles were detected conforming to lattice planes of Bragg's reflection (111), (200), (220) and (311) (Figure 4(E-H)) [24,38]. This is in agreement with the finding reported by Abbai et al, who characterized multifunctional Ag and AuNPs from the oriental herbal adaptogen Siberian ginseng [26].…”

Design Considerations and Assays for Hemocompatibility of FDA-Approved Nanoparticles

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