<i>In Vitro</i> and <i>In Vivo</i> Evaluation of Drug-Encapsulated Lignin Nanoparticles for Release Control

Lee, Jae Hoon; Im, Jun Su; Jin, Xuanjun; Kim, Tae Min; Weon, Choi Joon

doi:10.1021/acssuschemeng.1c08529

Cited by 20 publications

(17 citation statements)

References 39 publications

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“…Also, lignin has potential antioxidant and antimicrobial properties, and the structure can vary over a broad span depending on source and isolation process. For these reasons, lignin is rapidly gaining increasing interest from the scientific community as a potential controlled delivery carrier [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Spheronized drug microcarrier system from canola straw lignin

Zhang

Svärd

Edlund

2023

Science and Technology of Advanced Materials

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Inhomogeneous lignin from a canola (rapeseed) straw was isolated and valorized as regularly shaped spherical microparticles for drug delivery formulations. Lignin with a purity of 83% and broad molecular weight distribution (Ð > 5.0) was extracted by alkali pulping and acetylated to increase spheronization ability. Lignins with high degrees of acetylation (0.76 and 0.89) were successfully assembled into microparticles with uniform sizes (approximately 2 μm) and smooth spherical surfaces via solvent–antisolvent precipitation. Hydrophobic coumarin 153 and positively charged ciprofloxacin were used as model drugs to assess the encapsulation and release performance of lignin microparticles. Highly acetylated lignin microparticles displayed encapsulation efficiencies of 89.6% for coumarin 153% and 90.6% for ciprofloxacin. Scanning electron microscope images showed that coumarin 153 was encapsulated in the hydrophobic core, while ciprofloxacin was adsorbed on the less hydrophobic shell. The synthesis of lignin microcarriers not only provides a facile approach to utilizing waste canola straw lignin for drug delivery matrices but also has the potential to serve as an alternative lignin powder feedstock for bio-based materials.

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Section: Introductionmentioning

confidence: 99%

Spheronized drug microcarrier system from canola straw lignin

Zhang

Svärd

Edlund

2023

Science and Technology of Advanced Materials

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“…The prepared hydrogel can potentially help to decrease the exploitation and abuse of antibiotics . Henceforth, the release of RB from RBZPSH was studied under various physiological conditions, pH 7.4 and 5.5, designed to mimic the normal blood and microbial microenvironment/intracellular endosomal environment, respectively . To note, 90% of the RB was released at pH 5.5 during the first 6 h of the study from the RB-loaded ZPSH, and sustained release was observed for the next 18 h. However, less than 20% of the RB was released after 24 h at pH 7.4.…”

Section: Resultsmentioning

confidence: 99%

In Situ Nanoarchitectonics of a MOF Hydrogel: A Self-Adhesive and pH-Responsive Smart Platform for Phototherapeutic Delivery

Reddy

Paul

et al. 2023

Biomacromolecules

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Metal–organic frameworks (MOFs) have dramatically changed the fundamentals of drug delivery, catalysis, and gas storage as a result of their porous geometry, controlled architecture, and ease of postsynthetic modification. However, the biomedical applications of MOFs still remain a less explored area due to the constraints associated with handling, utilizing, and site-specific delivery. The major drawbacks associated with the synthesis of nano-MOFs are related to the lack of control over particle size and inhomogeneous dispersion during doping. Therefore, a smart strategy for the in situ growth of a nano-metal–organic framework (nMOF) has been devised to incorporate it into a biocompatible polyacrylamide/starch hydrogel (PSH) composite for therapeutic applications. In this study, the post-treatment of zinc metal ion cross-linked PSH with the ligand solution generated the nZIF-8@PAM/starch composites (nZIF-8, nano-zeolitic imidazolate framework-8). The ZIF-8 nanocrystals thus formed have been found to be evenly dispersed throughout the composites. This newly designed nanoarchitectonics of an MOF hydrogel was found to be self-adhesive, which also exhibited improved mechanical strength, a viscoelastic nature, and a pH-responsive behavior. Taking advantage of these properties, it has been utilized as a sustained-release drug delivery platform for a potential photosensitizer drug (Rose Bengal). The drug was initially diffused into the in situ hydrogel, and then the entire scaffold was analyzed for its potential in photodynamic therapy against bacterial strains such as E. coli and B. megaterium. The Rose Bengal loaded nano-MOF hydrogel composite exhibited remarkable IC50 values within the range of 7.37 ± 0.04 and 0.51 ± 0.05 μg/mL for E. coli and B. megaterium. Further, reactive oxygen species (ROS) directed antimicrobial potential was validated using a fluorescence-based assay. This smart in situ nanoarchitectonics hydrogel platform can also serve as a potential biomaterial for topical treatment including wound healing, lesions, and melanoma.

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“…Another strategy involves the development of lignin nanoparticles (LNPs) for loading compounds such as benzazulene (BZL) or the naturally renewable drug resveratrol. This methodology holds the potential to provide drug protection and enhance their anticancer effectiveness. , Additionally, the encapsulation of the anticancer drug adriamycin (DOX) in lignin nanoparticles resulted in enhanced utilization of DOX and improved anticancer effects. , Moreover, water-soluble modifications of hydrolyzed lignin using benzene poly(carboxylic acid) compounds (BP-Cx-1) have demonstrated multiple biological effects on neurotransmitter receptors, ligand-gated ion channels, and transporter proteins, thereby contributing to cancer treatment . Although these methods enhance drug availability and antitumor efficacy, lignin only serves as a passive carrier or active site provider, which limits its potential as a standalone antitumor biomaterial.…”

Section: Introductionmentioning

confidence: 99%

Antitumor Effects of Carrier-Free Functionalized Lignin Materials on Human Hepatocellular Carcinoma (HepG2) Cells

Wang,

Lu,

Liu

et al. 2024

ACS Nano

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Lignin, as an abundant aromatic biopolymer in plants, has great potential for medical applications due to its active sites, antioxidant activity, low biotoxicity, and good biocompatibility. In this work, a simple and ecofriendly approach for lignin fractionation and modification was developed to improve the antitumor activity of lignin. The lignin fraction KL-3 obtained by the lignin gradient acid precipitation at pH = 9−13 showed good cytotoxicity. Furthermore, the cell-feeding lignin after additional structural modifications such as demethylation (DKL-3), sulfonation (SL-3), and demethylsulfonation (DSKL-3) could exhibit higher glutathione responsiveness in the tumor microenvironment, resulting in reactive oxygen species accumulation and mitochondrial damage and eventually leading to apoptosis in HepG2 cells with minimal damage to normal cells. The IC 50 values for KL-3, SL-3, and DSKL-3 were 0.71, 0.57, and 0.41 mg/mL, respectively, which were superior to those of other biomass extractives or unmodified lignin. Importantly, in vivo experiments conducted in nude mouse models demonstrated good biosafety and effective tumor destruction. This work provides a promising example of constructing carrier-free functionalized lignin antitumor materials with different structures for inhibiting the growth of human hepatocellular carcinoma (HepG2) cells, which is expected to improve cancer therapy outcomes.

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In Vitro and In Vivo Evaluation of Drug-Encapsulated Lignin Nanoparticles for Release Control

Cited by 20 publications

References 39 publications

Spheronized drug microcarrier system from canola straw lignin

Spheronized drug microcarrier system from canola straw lignin

In Situ Nanoarchitectonics of a MOF Hydrogel: A Self-Adhesive and pH-Responsive Smart Platform for Phototherapeutic Delivery

Antitumor Effects of Carrier-Free Functionalized Lignin Materials on Human Hepatocellular Carcinoma (HepG2) Cells

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