In vitroandin vivotoxicity of pristine C₆₀fullerene aqueous colloid solution

Water-Soluble Pristine C60 Fullerenes Inhibit Liver Fibrotic Alteration and Prevent Liver Cirrhosis in Rats

“…Carbon-based nanostructure-C 60 fullerene-is one of the most powerful free radical scavengers [9,10]: 1 molecule of С 60 is able to bind up to 34 radicals depending on their size [11]. No toxic effect of water-soluble pristine C 60 fullerenes in the dose range of 75-150 mg/kg was observed on a mouse model (the LD 50 value was 721 mg/kg) [12]. Water-soluble C 60 fullerene is able to accumulate in rat liver [13] and prevent its acute toxic injury [14].…”

Water-Soluble Pristine C60 Fullerene Inhibits Liver Alterations Associated with Hepatocellular Carcinoma in Rat

“…However, if applied in doses less than 10–20 µg/mL (depending on cell line) C 60 fullerene didn’t increase the number of apoptotic and pre-apoptotic cells [ 46 ], being, therefore, relatively low-toxic. Low cytotoxicity of C 60 fullerene against normal and neoplastic cells was also demonstrated in [ 13 , 47 ]. Thus, C 60 fullerene did not affect leukemic cell viability when applying at 3.6–144 µg/mL (CCRF-CEM, Jurkat, Molt-16 cells), IC 50 for HEK293 cells was equal to 383.4 µg/mL.…”

“…It is important to note that C 60 FAS is a relatively safe substance: we did not observe any alterations of vital organs (liver, kidney, spleen, pancreas) or other signs of acute or subacute toxicity in animals receiving C 60 FAS for 7 weeks. Moreover, the maximum tolerated dose of C 60 fullerene was found to be 721 mg/kg for intraperitoneal administration to mice [ 13 ], and any significant violations appeared at the dose of C 60 FAS 150 mg/kg, which is significantly lower than that used in our experiment.…”

“…Furthermore, C 60 fullerene is nontoxic when used in therapeutic doses and can be accumulated in the liver [ 12 , 13 ], which makes it attractive to affect it. Hence, this nanoparticle is an ideal candidate for the prevention and treatment of liver disease associated with oxidative stress.…”

Activation of the Cold and Menthol Receptor TRPM8 Improves Post-Traumatic Recovery of Rat Muscle Soleus During Fullerene Treatment

“…Важно отметить, что в соответствии с нашими предыдущими исследованиями С 60 ÔВР не проявляют никаких токсических эффектов в системах in vitro (по крайней мере, при низких концентрациях) [19], а его максимальная переносимая доза составляет 721 мг/кг при внутрибрюшинном введении мышам [20].…”