<i>In vitro</i>and<i>in vivo</i>toxicity evaluation of halloysite nanotubes

Long, Zheru; Wu, Yanping; Gao, Huaying; Zhang, Jun; Ou, Xianfeng; He, Rong‐Rong; Liu, Mingxian

doi:10.1039/c8tb01382a

Cited by 96 publications

(51 citation statements)

References 60 publications

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“…Due to their morphological characteristics, halloysite nanotubes are efficient as catalytic supports [ 34 , 35 , 36 , 37 ], adsorbents of pollutants [ 38 , 39 ] and nanocarriers for functional molecules with biological [ 40 , 41 , 42 , 43 , 44 , 45 ] and chemical activities [ 46 , 47 , 48 , 49 ]. In this regards, it should be highlighted that halloysite is suitable for biomedical and pharmaceutical applications because of its biocompatibility and low toxicity, which was observed to both unicellular and multicellular organisms [ 50 , 51 , 52 , 53 , 54 ]. Interestingly, the inside/outside surfaces of halloysite are oppositely charged within the pH interval between 2 and 8 [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Halloysite Nanotubes Coated by Chitosan for the Controlled Release of Khellin

2020

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In this work, we have developed a novel strategy to prepare hybrid nanostructures with controlled release properties towards khellin by exploiting the electrostatic interactions between chitosan and halloysite nanotubes (HNT). Firstly, khellin was loaded into the HNT lumen by the vacuum-assisted procedure. The drug confinement within the halloysite cavity has been proved by water contact angle experiments on the HNT/khellin tablets. Therefore, the loaded nanotubes were coated with chitosan as a consequence of the attractions between the cationic biopolymer and the halloysite outer surface, which is negatively charged in a wide pH range. The effect of the ionic strength of the aqueous medium on the coating efficiency of the clay nanotubes was investigated. The surface charge properties of HNT/khellin and chitosan/HNT/khellin nanomaterials were determined by ζ potential experiments, while their morphology was explored through Scanning Electron Microscopy (SEM). Water contact angle experiments were conducted to explore the influence of the chitosan coating on the hydrophilic/hydrophobic character of halloysite external surface. Thermogravimetry (TG) experiments were conducted to study the thermal behavior of the composite nanomaterials. The amounts of loaded khellin and coated chitosan in the hybrid nanostructures were estimated by a quantitative analysis of the TG curves. The release kinetics of khellin were studied in aqueous solvents at different pH conditions (acidic, neutral and basic) and the obtained data were analyzed by the Korsmeyer–Peppas model. The release properties were interpreted on the basis of the TG and ζ potential results. In conclusion, this study demonstrates that halloysite nanotubes wrapped by chitosan layers can be effective as drug delivery systems.

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Section: Introductionmentioning

confidence: 99%

Halloysite Nanotubes Coated by Chitosan for the Controlled Release of Khellin

2020

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“…44,45 This was also confirmed in in vivo studies on nematodes 46 and zebrafish. 47 The effect of HNTs on cells is dose-dependent as has been shown for human cell lines. 48 Recently, the size-dependent character of cellular internalisation of HNTs (250-650 nm in length) has been demonstrated in colon cancer cells.…”

Section: Clay-based Nanomaterialsmentioning

confidence: 89%

Naturally derived nano- and micro-drug delivery vehicles: halloysite, vaterite and nanocellulose

et al. 2020

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“…The authors demonstrated that HNTs promote the hatchability of zebrafish embryos and did not affect the morphological development at a concentration of ≤25 mg mL −1 . HNTs accumulated predominantly in the gastrointestinal tract of zebrafish larvae similar to nematodes …”

Section: Biocompatibility Of Halloysite Nanotubesmentioning

confidence: 99%

Halloysite Nanoclay/Biopolymers Composite Materials in Tissue Engineering

Naumenko

Fakhrullin

2019

Biotechnology Journal

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Biocompatible materials for the fabrication of tissue substitutes are crucially important in the advancement of modern medicinal biotechnology. These materials, to serve their function, should be similar in physical, chemical, biological, and structural properties to native tissues which they are aimed to mimic. The porosity of artificial scaffolds is essential for normal nutrient transmission to cells, gas diffusion, and cell attachment and proliferation. Nanoscale inorganic additives and dopants are widely used to improve the functional properties of the polymer materials for tissue engineering. Among these inorganic dopants, halloysite nanotubes are arguably the most perspective candidates because of their biocompatibility and functional properties allowing to enhance significantly the mechanical and chemical stability of tissue engineering scaffolds. Here, this vibrant field of biotechnology for regenerative medicine is overviewed.

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In vitroandin vivotoxicity evaluation of halloysite nanotubes

Abstract: Halloysite is weakly toxic to cells and can be excreted out through the gastrointestinal metabolism of zebrafish.

Cited by 96 publications

References 60 publications

Halloysite Nanotubes Coated by Chitosan for the Controlled Release of Khellin

Halloysite Nanotubes Coated by Chitosan for the Controlled Release of Khellin

Naturally derived nano- and micro-drug delivery vehicles: halloysite, vaterite and nanocellulose

Halloysite Nanoclay/Biopolymers Composite Materials in Tissue Engineering

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