In vitroandin vivoeffects of progesterone onTrichinella spiralisnewborn larvae

Abstract

We have previously demonstrated that during pregnancy there exists an increased parasiticide activity against Trichinella spiralis newborn larvae (NBL) in infected rats. In this work we analysed the contribution of peritoneal cells from noninfected pregnant rats to the mortality of the NBL in cytotoxicity assays, and evaluated the role of progesterone in this effector mechanism. Our findings suggest that progesterone can induce activation of effector peritoneal cells to destroy the NBL in a rapid and antibody-independent manner. The administration of progesterone to ovariectomized rats also led to a significant decrease in the parasite load of the animals, thus demonstrating that progesterone induces the increase of the parasiticide activity of the leukocytes involved in the mechanisms of NBL death.

Variations in seroprevalences of canine leishmaniasis: Could it be a consequence of the population structure?

“…The clinical background and laboratory test results of the patients 3-6 involved in trichinellosis outbreaks have already been described in Nuñez et al, 2008. Briefly, the four women were young adults with no other clinical backgrounds (aged between 22 and 30 years old) from areas where historically occurs several outbreaks per year and swine, wild animals and pork products have high average parasite burden.…”

“…Serum sample from the other four patients were the same as those used by Nuñez et al, 2008. Also, serum samples from patient 4 and her infant were collected 8 years post-infection.…”

“…The detection of specific Abs, total Igs and Igs isotypes (IgE, IgG1 and IgG4) against T. spiralis was carried out in all serum samples by ELISA using muscle larvae excretory-secretory products (ML-ESP) as antigen (Ag), according to Nuñez et al, 2008. Serum samples were added diluted 1/200 for total Igs and 1/5 for IgE, IgG1 and IgG4.…”

“…Furthermore, little is known about the consequences of this infection in the course of pregnancy as well as its impact on the embryo/fetus development. Although transplacental passage of the migrant larvae or newborn larvae (NBL) has been reported by different authors in rats, mice, rabbits, ferrets and wild boars (Hartmanova and Chroust, 1968;Cosoroabã and Orjanu, 1998;Nuñez et al, 2002;Boulos et al, 2005;Wang ZQ et al, 2005;Webster and Kapel, 2005;Cohen et al, 2010), few cases have been studied in humans (Kuitunen-Ekbaum, 1941;Kociecka, 1981;Dubinský et al, 2001;Nuñez et al, 2008;Taybouavone et al, 2009) and in only one of these cases the presence of muscle larvae (ML) has been claimed in a human fetus (Dubinský et al, 2001).…”

Trichinella spiralis infection rapidly induces lung inflammatory response

“…The ML were obtained from the muscle tissue of Swiss mice by the artificial digestion method (Nuñez et al 2005). As controls, uninfected animals were administered PBS orally by gastric canula.…”