In vitroandin vivoantifungal activity of essential oils ofCymbopogon martiniandChenopodium ambrosioidesand their synergism against dermatophytes

Prasad, Chandra Shekhar; Shukla, Ravindra; Kumar, Ashok; Dubey, Nawal Kishore

doi:10.1111/j.1439-0507.2008.01676.x

Cited by 65 publications

(21 citation statements)

References 42 publications

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“…MIC of mixture of oils against T. rubrum was 0.02 μl/ml and M. gypseum was 0.04 μl/ml. Our findings are similar to Prasad et al 13 who found the synergistic antifungal efficacy of essential oils of Cymbopogon martini, Chenopodium ambrosiodies and their combinations against dermatophytes and some filamentous fungi in vitro. Our work also coincides with the findings of Cassella et al 14 studied the antifungal potential of tea tree and lavender essential oils alone and in combinations against T. rubrum and T. mentagrophytes and effective inhibition by mixture of oils than a single oils alone.…”

Section: Discussionsupporting

confidence: 92%

Synergistic Antifungal Activity ofCurcuma longa(Turmeric) andZingiber officinale(Ginger) Essential Oils Against Dermatophyte Infections

Sharma

2011

Journal of Essential Oil Bearing Plants

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The antifungal potential of Curcuma longa (turmeric) and Zingiber officinale (ginger) essential oils alone and in combinations, against common causes of tinea infections in humans was investigated via in vitro investigations, in order to determine a suitable dosage for use in clinical trials. Turmeric oil showed good antifungal activity (68 mm, MIC 1.6 μl/ml against Trichophyton rubrum and 5 6mm, MIC 1.4 μl/ml against Microsporum gypseum) while ginger oil had strong antifungal activity (72 mm, 0.05 μl/ml against T. rubrum and 69 mm, MIC 0.06 μl/ml against M. gypseum) and their mixture showed excellent antifungal activity (82 mm, MIC 0.02 μl/ml against T. rubrum and 79 mm, MIC 0.04 μl/ml against M. gypseum).The antifungal activity of oils was screened against T. rubrum and M. gypseum by using disc diffusion method and microdilution method. The inhibition zone of mixture of oils (turmeric and ginger) is higher than single oils and reference antibiotics i.e Clotrimazole (36 mm against T. rubrum, 41 mm against M. gypseum) and Ketoconazole (60 mm against T. rubrum, 26 mm against M. gypseum). From our findings, the results provide a scientific validation for the use of these essential oils in the treatment of dermatophytic infections and could be used in future for the development of anti-skin disease agents.

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Section: Discussionsupporting

confidence: 92%

Synergistic Antifungal Activity ofCurcuma longa(Turmeric) andZingiber officinale(Ginger) Essential Oils Against Dermatophyte Infections

Sharma

2011

Journal of Essential Oil Bearing Plants

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“…Studies report that the essential oil obtained from this species has antihelmintic activity (particularly against Ascaris lumbricoides), antifungal activity (Prasad, Shukla, Kumar, & Dubey, 2010), activity against Leishmanial (Monzote, García, Montalvo, Linares & Scull, 2009;Monzote, Nance, García, Scull & Setzer, 2011) and acaricide activity (Chiasson, Bostanian, & Vincent, 2004). In the present study, the essential oil from Chenopodium ambrosioides was extracted to determine the secretory structures and to evaluate the biological potential as an antioxidant and antibacterial agent.…”

Section: Introductionmentioning

confidence: 91%

Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities

Santiago

Cardoso

Batista

et al. 2016

Acta Sci. Biol. Sci.

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ABSTRACT. The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC 50 = 455.7 μg mL -1 . This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 μL mL -1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures.Keywords: natural products, antioxidant assays, antimicrobial, trichomes.Óleo essencial de Chenopodium ambrosioides L.: estruturas secretoras, atividade antibacteriana e antioxidante RESUMO. Objetivou-se neste trabalho avaliar as atividades antibacteriana e antioxidante do óleo essencial de Chenopodium ambrosioides L. e determinar as suas estruturas secretoras. A extração do óleo essencial foi realizada pelo método de hidrodestilação, utilizando o aparelho de Clevenger modificado com duração de 2 horas, sendo, posteriormente, analisado por CG/EM e CG-DIC. A atividade antioxidante foi determinada monitorando-se a redução do radical livre DPPH (2,2-difenil-1-picril-hidrazila) e utilizando o ensaio de oxidação do sistema β-caroteno/ácido linoleico. A avaliação da atividade antibacteriana foi realizada por meio da técnica difusão cavidade em ágar, utilizando os micro-organismos Staphylococcus aureus, Listeria monocytogenes, Escherichia coli e Salmonella Cholerasuis. A caracterização dos tricomas foi realizada por Microscopia Eletrônica de Varredura (MEV) e pelos testes histoquímicos com os reagentes de Nadi e Sudan IV. A atividade antioxidante foi evidenciada pelo teste β-caroteno/ácido linoleico, com CI 50 455,7 μg mL -1. O óleo também apresentou atividade antibacteriana tanto para bactérias Gram-negativas como para Gram-positivas. A concentração mínima inibitória do mesmo variou entre 62,5 e 250 μL mL -1. Constatouse a presença de compostos terpênicos nos tricomas glandulares, inferindo-se que o óleo essencial seja secretado nessas estruturas.Palavras-chave: produtos naturais, ensaios antioxidantes, antimicrobiano, tricomas.

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“…It is worth noting that volatile oils containing CIT, GOL and NOL, have proved promising, and may be incorporated into pharmaceutical preparations, alone or in combination (Prasad et al, 2010;Saddiq and Khayyat, 2010;Zore et al, 2011a;Miron et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts

Miron¹,

Battisti²,

Silva³

et al. 2014

Revista Brasileira de Farmacognosia

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GeraniolNerol Isomers Microdilution assay Mechanism of action A B S T R A C T Dermatomycosis causes highly frequent dermal lesions, and volatile oils have been proven to be promising as antifungal agents. The antifungal activity of geraniol, nerol, citral, neral and geranial (monoterpenes), and terbinafine and anidulafungin (control drugs) against seven opportunistic pathogenic yeasts and four dermatophyte species was evaluated by the Clinical and Laboratory Standards Institute microdilution tests. Monoterpenes were more active against dermatophytes than yeasts (geometric mean of minimal inhibitory concentration (GMIC) of 34.5 and 100.4 μg.ml -1 , respectively). Trichophyton rubrum was the fungal species most sensitive to monoterpenes (GMIC of 22.9 μg.ml -1 ). The trans isomers showed higher antifungal activity than the cis. The mechanism of action was investigated evaluating damage in the fungal cell wall (Sorbitol Protection Assay) and in the cell membrane (Ergosterol Affinity Assay). No changes were observed in the MIC of monoterpenes in the sorbitol protection assay. The MIC of citral and geraniol was increased from 32 to 160 μg.ml -1 when the exogenous ergosterol concentrations was zero and 250 μg.ml -1 , respectively. The monoterpenes showed an affinity for ergosterol relating their mechanism of action to cell membrane destabilization.

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In vitroandin vivoantifungal activity of essential oils ofCymbopogon martiniandChenopodium ambrosioidesand their synergism against dermatophytes

Cited by 65 publications

References 42 publications

Synergistic Antifungal Activity ofCurcuma longa(Turmeric) andZingiber officinale(Ginger) Essential Oils Against Dermatophyte Infections

Synergistic Antifungal Activity ofCurcuma longa(Turmeric) andZingiber officinale(Ginger) Essential Oils Against Dermatophyte Infections

<b>Essential oil from <i>Chenopodium ambrosioides</i> L.: secretory structures, antibacterial and antioxidant activities

Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts

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