In vitro and in vivo antitumor activity of liposomal fenretinide targeted to human neuroblastoma

The chemopreventive agent N-(4-hydroxyphenyl)retinamide induces apoptosis through a mitochondrial pathway regulated by proteins from the Bcl-2 family

“…Introduction N-(4-hydroxyphenyl)retinamide (4-HPR, fenretinide) has potent chemopreventive and antimetastatic effects in several animal models (Green et al, 1999;Shaker et al, 2000;Hursting et al, 2001;Raffaghello et al, 2003) and is currently undergoing clinical trials for the prevention of ovarian carcinoma (De Palo et al, 2002), lung carcinoma (Cai and Jones, 1998), and breast neoplasia (Levi et al, 2001;Singletary et al, 2001). In addition, 4-HPR can exert chemotherapeutic effects and kill cancer cells in a variety of in vitro and in vivo models, through the induction of apoptosis (Wu et al, 2001;Fontana and Rishi, 2002).…”

Anti-GD2 MABS and Next-Generation mAb-Based Agents for Cancer Therapy

“…Different nanoparticle formats that have been bioconjugated with anti-GD2 full size mAbs or Fab fragments include: liposomes, which have small diameter and are able to differentially accumulate and penetrate into solid tumors (with highly permeable capillaries) relative to normal tissue (with less permeable tight junctions) [121][122][123][124]; porous silica nanoparticles, which are inert, biodegradable, nontoxic and more stable due to uniform particle size [125]; gold nanorods and carbon nanotubes capable of absorbing near-infrared laser light leading to in vitro photothermal destruction of tumor cells [126,127]. ; and iron-based nanoparticles that enable direct delivery of drugs c arried by the nanoparticle [128].…”

“…There have been many studied, including: doxorubicin (chemotherapeutic drug) [122]; antisense oligonucleotide (downregulate c-myb and c-myc proto-oncogenes) [121,123]. ; fenretinide (HPR), a synthetic retinoid acid derivative that promotes apoptosis of NBL cells in vitro [124]; miR-34a (multi-gene targeting microRNA capable activating caspase-mediated apopototic pathways) [125]. Some of these targeted nanoparticles showed p romising a ntitumor effects in vivo.…”

In vitro and In vivo Antitumor Activity of the Novel Derivatized Polyvinyl Alcohol-Based Polymer P10(4)

“…Moreover, this strategy also contributes to improve the solubility in biological fluids of several mildly to highly lipophilic drugs (5). Several approaches have been undertaken with different chemical composition of these drug vehicles, with liposomes, and their actively targeting form (immunoliposomes), being perhaps the most suitable (12,18). An extensive review of the several approaches to nanoparticlemediated drug targeting is provided by Moghimi et al (9).…”

“…For therapeutic experiments, CD1 nude/nude mice were injected i.v. with HTLA-230 (5 Â 10 6 per mouse; 12 mice per group) and NXS2 (100,000 per mouse; 9 mice per group) as described (18). Twenty-four hours after the tumor cell inoculation, the animals were treated with P10(4) (300 mg/kg) or vehicle alone (PBS), given slowly through the tail vein in a volume of 200 AL.…”