In vitro
 and
 in vivo
 evaluation of antimicrobial activity of
 Zooshikella marina
 against pathogenic bacteria causing vibriosis in aquaculture

Puangpee, Sumesa; Suanyuk, Naraid

doi:10.1111/are.15371

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The pathogenicity of V. parahaemolyticus is closely related to a variety of virulence factors, including the iron absorption system, lipopolysaccharides, proteases, outer membrane proteins, the adhesion factor type III secretion system, and the type VI secretion system ( He et al, 2020 ; Puangpee and Suanyuk, 2021 ). Biofilms are an important survival and pathogenic mechanism of V. parahaemolyticus , which are difficult to remove ( Hall-Stoodley et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Liu

Wang

2022

Front. Microbiol.

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This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its possible antibiofilm mechanisms using transcriptomic analysis. The results indicated that the minimum antibacterial concentration of PCA against V. parahaemolyticus was 300 μg/mL. PCA (9.375 μg/mL) inhibited biofilm generation and adhesion of the mature biofilm. PCA (75 μg/mL) significantly reduced the metabolic viability of V. parahaemolyticus, reduced polysaccharide production, and inhibited cell surface flagella-mediated swimming and aggregation phenotypes. Meanwhile, transcriptome analysis showed that the key genes of V. parahaemolyticus expressed under PCA (75 μg/mL) inhibition were mainly related to biofilm formation (pfkA, galE, narL, and oppA), polysaccharide production and adhesion (IF, fbpA, and yxeM), and motility (cheY, flrC, and fliA). By regulating these key genes, PCA reduced biofilm formation, suppressed polysaccharide production and transport, and prevented the adhesion of V. parahaemolyticus, thereby reducing the virulence of V. parahaemolyticus. This study demonstrated that protocatechuic aldehyde can be used to control V. parahaemolyticus biofilm to ensure food safety.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Liu

Wang

2022

Front. Microbiol.

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“…Piscidin-4 can bind to the lipopolysaccharide on the outside of the bacterial membrane via an electrostatic gravitational force, disturbing the cell wall in that area and altering its stability, which allows it to cross the area smoothly, come to the proximity of the cell membrane, and eradicate the microbial biofilm. Piscidin-4 was highly active against Gram-negative bacteria, including Vibrio spp., that pose a serious threat to aquaculture and possessed immunomodulatory properties [ 9 , 10 ]. Different studies have shown that both AMPs, NZ2114 and Piscidin-4, have weak host toxicity and low hemolytic activity against vertebrates, suggesting that they are more suitable for antibacterial studies [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Han

Zhao²,

Liu

et al. 2022

Marine Drugs

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To solve the problem of antibiotic abuse in aquaculture and to utilize the application potential of antimicrobial peptides (AMPs), a chloroplast transformation system of Porphyridium purpureum was successfully constructed for effectively expressing two exogenous AMPs. The endogenous fragments of 16S rDNA/trnA-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter psbB in a polycistron. The plasmids were transferred into P. purpureum via particle bombardment and the transformation vectors were screened using phosphinothricin (bar), a dominant selection marker under the control of the psbA promoter. Subsequently, in the positive transformed colonies, the exogenous fragments were found to be inserted in the flanking fragments directionally as expected and two foreign AMPs were successfully obtained. Finally, two exogenous peptides with antibacterial properties were obtained from the transformed strain. The two AMPs expressed by the transformed strain were shown to have similar inhibitory effects to antibiotics by inhibition tests. This suggested that AMPs can be introduced into aquaculture using baited microalgae, providing new ideas and ways to solve a series of aquaculture diseases caused by bacteria.

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Antibacterial activity and virulence attenuation of peptides-zinc nanocomposite against Vibrio alginolyticus

Zheng,

Lin,

et al. 2024

Food Bioscience

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In vitro and in vivo evaluation of antimicrobial activity of Zooshikella marina against pathogenic bacteria causing vibriosis in aquaculture

Cited by 4 publications

References 39 publications

Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Antibacterial activity and virulence attenuation of peptides-zinc nanocomposite against Vibrio alginolyticus

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