In vitro and in silico screening of novel typhoid drugs from endangered herb (Enicostema axillare)

Sakthivel, P.; Samy, MadhanaVigneshwari Gopal; Dharaneedharan, Subramanian

doi:10.1080/07391102.2022.2041488

Cited by 3 publications

(1 citation statement)

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“…Isolated and purified swertiamarin from Enicostema axillare was dissolved in double distilled water in the concentration of 10 mg/ml as stock (Perumal et al, 2022).…”

Section: Test Compoundmentioning

confidence: 99%

Effect of novel therapeutic medicine swertiamarin from Enicostema axillare in zebrafish infected with Salmonella typhi

2022

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Herbal treatments have been practiced by humans over centuries and therefore possess time‐proven safety. However, it is crucial to evaluate the toxic effects of herbal medicine to confirm their safety, particularly when developing therapeutic drugs. Use of laboratory animals such as mice, rat, and rabbits was considered as gold standard in herbal toxicity assessments. However, in the last few decades, the ethical consideration of using higher vertebrates for toxicity testing has become more controversial. As a possible alternative model involving lower vertebrates such as zebra fish were introduced. Hence in the present study, swertiamain compound isolated from E. axillare was assessed for it antimicrobial activity in zebra fish larvae againt S. typhi. The cumulative mortality rate and bacterial localization in zebra fish larvae were studied. Biochemical markers assays were performed to find the preventive role of the compound during the typhoid infection. The results showed that zebra fish can be successfully used as a model to study typhoid infection and the anti‐bacterial compound swertiamarin used in this study clears the bacterial load and pathogenic symptoms to a great extent.

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“…Isolated and purified swertiamarin from Enicostema axillare was dissolved in double distilled water in the concentration of 10 mg/ml as stock (Perumal et al, 2022).…”

Section: Test Compoundmentioning

confidence: 99%