<i>In vitro</i> analysis of the penetration of methylene blue dye in human radicular dentin using different methods of impregnation

Ferreira, Ronise; Bombana, Antônio Carlos; Sayeg, Isaac Jamil

doi:10.1111/j.1747-4477.2007.00107.x

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…One reason for such a phenomenon might be the presence of air bubbles that can inhibit the penetration process (1,11). Also, it has been shown that the type of dye, methylene blue or fuchsine, or even the vacuum conditions don't change the amount of dye penetration (12,13). In the present study, we placed dye on top of the root end filling material to take advantage of gravity force.…”

Section: Discussionmentioning

confidence: 94%

Comparison of Pro Root Mineral Trioxide Aggregate and Calcium Enriched Mixture Cement Microleakage as Root End Filling Material: An in Vitro Analysis of Dye Penetration

Faramarzi¹,

Vossoghi²,

Shams³

et al. 2015

Avicenna J Dent Res

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Background: Sealing ability is an essential factor for retrograde filling material in successful endodontic apical surgery. The aim of this in vitro study was to evaluate dye microleakage of Mineral Trioxide Aggregate (MTA) and Calcium Enriched Mixture (CEM cement) as root end filling material. Objectives: The aim of this in vitro study was to compare sealing ability of these two root end filling materials using the dye penetration method. Materials and Methods: Eighty-six single rooted teeth were randomly divided into two study (n = 40) groups and two positive and negative control (n = 3) groups. After decoronation closely to the cementum enamel junction, the root canals were shaped by the crown down technique and obturated by gutta-percha and AH26 sealer with the lateral condensation method. Furthermore, 3 mm of root ends were resected and 3 mm root-end cavities were prepared by ultrasonic microsurgical tip. Root-end cavities were filled with each of the mentioned materials. Methylene blue dye was used for determination of dye leakage. Chi-2 analysis and t-test were used to compare groups. Results: The mean apical penetration of dye in the MTA group was 0.94 mm while in the CEM cement group this was 1.17 mm. Eighty percent of the MTA group and 75% of the CEM cement group didn't have any dye leakage. The P level was greater than 0.05 (0.592). Conclusions: There was no significant statistical difference between MTA and CEM cement in sealing ability (P > 0.05).

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Section: Discussionmentioning

confidence: 94%

Comparison of Pro Root Mineral Trioxide Aggregate and Calcium Enriched Mixture Cement Microleakage as Root End Filling Material: An in Vitro Analysis of Dye Penetration

Faramarzi¹,

Vossoghi²,

Shams³

et al. 2015

Avicenna J Dent Res

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“…Yet, there have been disparities in these trials 7 have been conflicting. 8 In the existing study, SEM-a method of histological evaluation has been used to assess the root canal seal quality. Scanning Electron Microscope has greater resolution, improved interface magnification, and superior field depth, which has also been stated by Punithia and Shashikala.…”

Section: Discussionmentioning

confidence: 99%

A Scanning Electron Microscope Analysis of Sealing Potential and Marginal Adaptation of Different Root Canal Sealers to Dentin: An In Vitro study

Patri

Agrawal

Anushree

et al. 2020

The Journal of Contemporary Dental Practice

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Aim: The present study aimed to evaluate the sealing potential and marginal adaptation of different root canal sealers to dentin. Materials and methods: A total of sixty human lower premolars of the permanent dentition that were extracted were used for this study. The visible debris and calculus were removed from the extracted teeth ultrasonically and were kept for 2 hours in 2.5% sodium hypochlorite and stored in normal saline till next use. A low-speed diamond disc was used to section all the teeth samples at the cementoenamel junction. Later, cleaning and shaping of the canals was done. Based on the sealer used, the samples (each group consisting of 20 samples) were divided randomly into three groups: group I-bioceramic sealer, group II-resin-based sealer, group III-MTA-based sealer. All split samples were visualized under scanning electron microscope (SEM) at apical and coronal thirds of root canal, the marginal gap at root dentin and sealer interface were assessed. Results: The highest marginal adaptation (5.60 ± 0.12) was demonstrated by EndoSequence BC sealer, followed immediately by ProRoot MTA sealer (4.48 ± 0.12) and EndoREZ sealer (2.10 ± 0.54). A statistically significant difference (p = 0.001) was seen between the EndoSequence BC and ProRoot MTA sealer for apical and coronal marginal adaptation. Also, a statistically significant difference (p < 0.05) was found between EndoSequence BC sealer vs EndoREZ sealer at coronal and EndoSequence BC sealer vs EndoREZ sealer and EndoREZ sealer vs ProRoot MTA sealer at apical third. Conclusion: The present study concluded that significant and better sealing ability and marginal adaptation was demonstrated by EndoSequence BC (bioceramic sealer) when compared to ProRoot MTA sealer (MTA-based sealer) and EndoREZ sealer (resin-based sealer). Clinical significance: Numerous endodontic sealers enter the market with various factors to attain acceptable seal. According to current study bioceramic sealer, is the appropriate sealer that hermetically seals all the margins.

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“…In the gap between dentin and filling materials, methylene blue solution penetrates into dentin tubules easier than do filling materials. Therefore, dentin in contact with filling materials was dyed with methylene blue solution 31) (Figs. 3a, 4a, 5a, and 6a).It is speculated that there is a pathway around the dyed dentin 32) .…”

Section: Observation Of Sectionsmentioning

confidence: 99%

Evaluation of coronal leakage and pathway of dye leakage after obturation with various materials for open apical foramina

et al. 2013

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The aim of this study was to investigate coronal leakage after obturation with mineral trioxide aggregate (MTA), resin-based sealer, and silicon-based sealer for open apical foramina and to evaluate pathway of leakage. Twenty-eight maxillary premolars were used, and instrumented to ISO size #80. Teeth were randomly divided into four groups as follows: Group A filled with MTA, Group B with gutta-percha and resin-based sealer, Group C with polymer-based material and resin-based sealer, and Group D with gutta-percha and silicon-based sealer. All samples were evaluated for coronal leakage with methylene blue solution and spectrophotometry. After leakage testing, samples were cut, and sections were observed. Dye leakage of Group A was significantly lowest among all groups at 15 days and 30 days. Defects which induced coronal leakage in resin-based sealer were observed at 7 mm from the apex. Coronal leakage after obturation with MTA for open apical foramina was significantly lower than resin-based sealer and silicon-based sealer.

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In vitro analysis of the penetration of methylene blue dye in human radicular dentin using different methods of impregnation

Cited by 6 publications

References 10 publications

Comparison of Pro Root Mineral Trioxide Aggregate and Calcium Enriched Mixture Cement Microleakage as Root End Filling Material: An in Vitro Analysis of Dye Penetration

Comparison of Pro Root Mineral Trioxide Aggregate and Calcium Enriched Mixture Cement Microleakage as Root End Filling Material: An in Vitro Analysis of Dye Penetration

A Scanning Electron Microscope Analysis of Sealing Potential and Marginal Adaptation of Different Root Canal Sealers to Dentin: An In Vitro study

Evaluation of coronal leakage and pathway of dye leakage after obturation with various materials for open apical foramina

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