In vitro Activity of Impatiens balsamina L. Against Multiple Antibiotic-Resistant Helicobacter pylori

Abstract

Impatiens balsamina L. has been used as indigenous medicine in Asia for the treatment of rheumatism, fractures, and fingernail inflammation. In this study, anti-H. pylori activity of I. balsamina L. was investigated. The MICs, MBCs, time-kill assay, and effect of environmental pH for the plant extracts were determined. The test H. pylori strains have resistance to clarithromycin (CLR), metronidazole (MTZ), and levofloxacin (LVX). From our results, all part (root/stem/leaf, seed, and pod) extracts of I. balsamina L. exhibited bactericidal H. pylori activity. Specifically, the pod extract had significantly lower MICs and MBCs (1.25-2.5 and 1.25-5.0 microg/ml, respectively). Of the five pod-extraction solvents, both ethyl acetate and acetone were the most efficient for the anti-H. pylori compounds of the pod extraction. The dose-dependency of the pod extract's bactericidal activity was H. pylori strain-dependent. Bactericidal H. pylori activity of the pod extract was not affected by the environmental pH (2-8). In summary, the acetone and ethyl acetate pod extracts of I. balsamina L. exhibited very strong anti-H. pylori activity. This activity exceeded that of MTZ and approximated to that of AMX.

Adverse Effects of Hydroalcoholic Extracts and the Major Components in the Stems of Impatiens balsamina L. on Caenorhabditis elegans

“…I. balsamina stems have long been used in TCM for the treatment of a range of diseases and ailments [7, 13, 14, 49–57]. However, there have been very few studies regarding its potential toxicity [16, 58–63].…”

Impatiens balsamina

“…All parts (root/stem/leaf, seed and pod) of I. balsamina extracts exhibited bactericidal activity against multiple antibiotic-resistant Helicobacter pylori (Wang et al 2009 ). Specifi cally, the pod extract had signifi cantly lower MICs and MBCs (1.25-2.5 and 1.25-5.0 μg/ml, respectively).…”

“…Impatiens balsamina has been used as an indigenous medicine in Asia for the treatment of rheumatism, fractures and fi ngernail infl ammation (Wang et al 2009 ). The plant is cathartic, diuretic and emetic and prescribed for treatment of pains in the joints (Chopra et al 1986 ).…”

Antioxidant, Antibacterial and Antifungal Activity of Impatiens sulcata Wallich in Roxb. Extracts

“…The secondary metabolites isolated from Impatiens species include phenolics, flavonols, anthocyanin pigments, quinones and saponins [2][3]. Impatiens species are reported to be useful as antifungal [4], antibacterial [5], anti-anaphylactic [6], anti-inflammatory [7], anti-pruritic/anti-dermatitis [8], anti-tumour [9], antibacterial [10], anti-helminthic [11] and anti-histaminic [12]. The present paper for first time reports the antioxidant potential and antimicrobial activity of Impatiens sulcata from the high altitude regions of North West (Garhwal) Himalayas, Uttarakhand, India.…”