<i>In vitro</i> activation of the classical pathway of complement by a streptococcal lipoteichoic acid

Monefeldt, Kari; Helgeland, Kristen; Tollefsen, Tore

doi:10.1111/j.1399-302x.1994.tb00037.x

Cited by 8 publications

(3 citation statements)

References 24 publications

(23 reference statements)

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“…The importance of the negative charge in LTA binding for the activation of the classical complement pathway has been described previously (27,28). The degree of interaction with C1 and its subcomponent C1q, which also contains short collagenous domains (1), was shown to depend upon the presence of the negative charges of the phosphate groups of LTA.…”

Section: Discussionmentioning

confidence: 77%

Influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor

1996

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Lipoteichoic acids (LTAs) belong to the immunostimulatory class of molecules of gram-positive bacteria (GPB). Previous investigations showed that the macrophage scavenger receptor (SR), a glycosylated trimeric transmembrane protein, binds directly to many GPB, possibly via LTA. SR binding to other ligands is dependent upon the spatial characteristics of the repeating negative charge of the ligand. We therefore investigated SR recognition of LTA species with various charge densities and distributions by determining the capacity of these LTAs to compete with the binding of metabolically labeled SR to beads coated with the known SR ligand polyguanylic acid. Staphylococcus aureus LTA, a classical LTA type (unbranched 1,3-linked polyglycerophosphate chain covalently bound to a membrane diacylglyceroglycolipid), had a 50% inhibitory concentration (IC 50) for inhibition of SR binding of 0.84 g/ml. When the S. aureus LTA was rendered more negatively charged by removal of ester-linked alanine from the polyglycerophosphate backbone, the IC 50 dropped to 0.23 g/ml. Other polyglycerophosphate LTAs from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Listeria monocytogenes, Listeria welshimeri, and Streptococcus sanguis showed IC 50 s of 0.5 to 2.1 g/ml, supporting the role of negative charge in binding to SR. Accordingly, the zwitterionic LTA of Streptococcus pneumoniae and Clostridium innocuum LTA substituted with positively charged sugar residues had no binding capacity. Monoglycerophosphate branches, but not succinyl ester, affected binding of lipoglycans. The data presented above parallel the previous findings for whole organisms and support the role of surface-associated LTA as a major ligand of GPB for binding to SR. Whether binding of LTA and whole GPB to macrophages initiates uptake and degradation or results in signal transduction remains to be determined.

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Section: Discussionmentioning

confidence: 77%

Influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor

1996

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“…LTA is a major cell wall component of gram-positive bacteria [75,76]. In human, the classical complement pathway is activated by LTA in vitro [77]. In addition, immunity is related to antioxidative status in fish [78].…”

Section: Discussionmentioning

confidence: 99%

The effect of dietary supplementation with Clostridium butyricum on the growth performance, immunity, intestinal microbiota and disease resistance of tilapia (Oreochromis niloticus)

Zhou²,

Ling³

et al. 2019

PLoS ONE

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This study was conducted to assess the effects of dietary Clostridium butyricum on the growth, immunity, intestinal microbiota and disease resistance of tilapia (Oreochromis niloticus). Three hundreds of tilapia (56.21 ± 0.81 g) were divided into 5 groups and fed a diet supplemented with C. butyricum at 0, 1 x 104, 1 x 105, 1 x 106 or 1 x 107 CFU g-1 diet (denoted as CG, CB1, CB2, CB3 and CB4, respectively) for 56 days. Then 45 fish from each group were intraperitoneally injected with Streptococcus agalactiae, and the mortality was recorded for 14 days. The results showed that dietary C. butyricum significantly improved the specific growth rate (SGR) and feed intake in the CB2 group and decreased the cumulative mortality post-challenge with S. agalactiae in the CB2, CB3 and CB4 groups. The serum total antioxidant capacity and intestinal interleukin receptor-associated kinase-4 gene expression were significantly increased, and serum malondialdehyde content and diamine oxidase activity were significantly decreased in the CB1, CB2, CB3 and CB4 groups. Serum complement 3 and complement 4 concentrations and intestinal gene expression of tumour necrosis factor α, interleukin 8, and myeloid differentiation factor 88 were significantly higher in the CB2, CB3 and CB4 groups. Intestinal toll-like receptor 2 gene expression was significantly upregulated in the CB3 and CB4 groups. Dietary C. butyricum increased the diversity of the intestinal microbiota and the relative abundance of beneficial bacteria (such as Bacillus), and decreased the relative abundance of opportunistic pathogenic bacteria (such as Aeromonas) in the CB2 group. These results revealed that dietary C. butyricum at a suitable dose enhanced growth performance, elevated humoral and intestinal immunity, regulated the intestinal microbial components, and improved disease resistance in tilapia. The optimal dose was 1 x 105 CFU g-1 diet.

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“…In addition to binding to endotoxin from Gram-negative bacteria, C1 may bind to streptococcal lipoteichoic acid [14]. These may have potentially important functional consequences that are not dependent on protease inhibition.…”

Section: Sepsis and Complementmentioning

confidence: 99%

Bench-to-bedside review: The role of C1-esterase inhibitor in sepsis and other critical illnesses

Singer

Jones²

2010

Critical Care

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In vitro activation of the classical pathway of complement by a streptococcal lipoteichoic acid

Cited by 8 publications

References 24 publications

Influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor

Influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor

The effect of dietary supplementation with Clostridium butyricum on the growth performance, immunity, intestinal microbiota and disease resistance of tilapia (Oreochromis niloticus)

Bench-to-bedside review: The role of C1-esterase inhibitor in sepsis and other critical illnesses

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