In situ13CO2 pulse‐labeling in a temperate heathland – development of a mobile multi‐plot field setup

Reinsch, Sabine

doi:10.1002/rcm.6584

Cited by 8 publications

(7 citation statements)

References 47 publications

(161 reference statements)

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“…The three-factorial treatment combination of warming, drought and elevated CO2 is not expected to cause significant changes of the SOM pools after eight treatment years. Previous shorter term experiments at the specific site showed that the stimulating effects of elevated CO2 and warming on plant biomass, SOM turnover (measured via soil and leaf litter incubation bags after 1 year) and soil fauna cancelled out or were 5 reduced when combined with drought (Andresen et al 2010;Kongstad et al 2012;Larsen et al 2011;Maraldo et al 2010;Reinsch & Ambus 2013).…”

mentioning

confidence: 91%

Decrease in heathland soil labile organic carbon under future atmospheric and climatic conditions

2017

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confidence: 91%

Decrease in heathland soil labile organic carbon under future atmospheric and climatic conditions

2017

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“…Unfortunately, this cannot be achieved due to the limitations of the used IRMS. Reinsch and Ambus adjusted resistor settings of the mass spectrometer to obtain higher measurement precision, but still they found that x ( 13 C) values measured by IRMS for 13 C-enriched samples ( x ( 13 C) = 50%) were also significantly dependent on CO 2 concentrations . They subsequently used an empirical relationship to correct data but the measured x ( 13 C) values never showed the expected values.…”

Section: Resultsmentioning

confidence: 99%

Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy

Dong

Jochmann

Elsner

et al. 2017

Environ. Sci. Technol.

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Assessing the biodegradation of organic compounds is a frequent question in environmental science. Here, we present a sensitive, inexpensive, and simple approach to monitor microbial mineralization using reverse stable isotope labeling analysis (RIL) of dissolved inorganic carbon (DIC). The medium for the biodegradation assay contains regular organic compounds and 13C-labeled DIC with 13C atom fractions (x(13C)DIC) higher than natural abundance (typically 2–50%). The produced CO2 (x(13C) ≈ 1.11%) gradually dilutes the initial x(13C)DIC allowing to quantify microbial mineralization using mass-balance calculations. For 13C-enriched CO2 samples, a newly developed isotope ratio mid-infrared spectrometer was introduced with a precision of x(13C) < 0.006%. As an example for extremely difficult and slowly degradable compounds, CO2 production was close to the theoretical stoichiometry for anaerobic naphthalene degradation by a sulfate-reducing enrichment culture. Furthermore, we could measure the aerobic degradation of dissolved organic carbon (DOC) adsorbed to granular activated carbon in a drinking water production plant, which cannot be labeled with 13C. Thus, the RIL approach can be applied to sensitively monitor biodegradation of various organic compounds under anoxic or oxic conditions.

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“…These studies have also shown that this flux might be affected by a range of factors such as the seasonality of plant activity. Usually, more carbon is allocated below‐ground towards the end of the growing season (Balasooriya et al., ; Högberg et al., ), under exposure to elevated atmospheric CO 2 concentrations (Jin & Evans, ; Reinsch & Ambus, ), under drought conditions (Fuchslueger, Bahn, Fritz, Hasibeder, & Richter, ) or in plants grown on fertile soils (Denef, Roobroeck, Manimel Wadu, Lootens, & Boeckx, ).…”

Section: Methods Using In‐situ 13c Labelling To Study Rhizodepositionmentioning

confidence: 99%

“…Therefore, a mobile flow-through system suitable for continuous 13 C-CO 2 delivery was developed (Box 3): A gas-tight vinyl balloon (c. 3 m diameter) was filled with CO 2 free synthetic air and mixed with 13 C-CO 2 (50 or 99 atom%) that supplied the transparent chambers enclosing the vegetation of interest with air over the duration of the experiments, ranging from 4 to 7.5 hr. Air was pumped continuously through gas tight tubing via electric diaphragm pumps (Reinsch & Ambus, 2013). The first experiment was conducted at the end of the growing season (October 2010), when we observed the highest allocation of carbon below-ground as measured by 13 C in soil respiration (Reinsch et al, 2014).…”

Section: C-co 2 Pulse Labellingmentioning

confidence: 99%

“…Thus, the analysis of the natural abundance of carbon isotopes in these compartments can give information about some processes related to photosynthesis and carbon losses through plant or soil respiration. In addition, in situ pulse labelling with the heavy stable carbon isotope ( 13 C) is a powerful tool to analyse short‐term dynamics of carbon allocation to the soil with high resolution (Epron et al., ; Högberg et al., ; Reinsch & Ambus, ). The application of these isotopic methods can therefore provide unique information about above‐ground‐below‐ground linkages and their alterations in response to climate changes.…”

Section: Introductionmentioning

confidence: 99%

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Isotopic methods for non‐destructive assessment of carbon dynamics in shrublands under long‐term climate change manipulation

et al. 2018

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Long‐term climate change experiments are extremely valuable for studying ecosystem responses to environmental change. Examination of the vegetation and the soil should be non‐destructive to guarantee long‐term research. In this paper, we review field methods using isotope techniques for assessing carbon dynamics in the plant–soil–air continuum, based on recent field experience and examples from a European climate change manipulation network. Eight European semi‐natural shrubland ecosystems were exposed to warming and drought manipulations. One field site was additionally exposed to elevated atmospheric CO2. We discuss the isotope methods that were used across the network to evaluate carbon fluxes and ecosystem responses, including: (1) analysis of the naturally rare isotopes of carbon (13C and 14C) and nitrogen (15N); (2) use of in situ pulse labelling with 13CO2, soil injections of 13C‐ and 15N‐enriched substrates, or continuous labelling by free air carbon dioxide enrichment (FACE) and (3) manipulation of isotopic composition of soil substrates (14C) in laboratory‐based studies. The natural 14C signature of soil respiration gave insight into a possible long‐term shift in the partitioning between the decomposition of young and old soil carbon sources. Contrastingly, the stable isotopes 13C and 15N were used for shorter‐term processes, as the residence time in a certain compartment of the stable isotope label signal is limited. The use of labelled carbon‐compounds to study carbon mineralisation by soil micro‐organisms enabled to determine the long‐term effect of climate change on microbial carbon uptake kinetics and turnover. Based on the experience with the experimental work, we provide recommendations for the application of the reviewed methods to study carbon fluxes in the plant–soil–air continuum in climate change experiments. 13C‐labelling techniques exert minimal physical disturbances, however, the dilution of the applied isotopic signal can be challenging. In addition, the contamination of the field site with excess 13C or 14C can be a problem for subsequent natural abundance (14C and 13C) or label studies. The use of slight changes in carbon and nitrogen natural abundance does not present problems related to potential dilution or contamination risks, but the usefulness depends on the fractionation rate of the studied processes.

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In situ¹³CO₂ pulse‐labeling in a temperate heathland – development of a mobile multi‐plot field setup

Abstract: A mobile flow-through system suitable for continuous in situ (13)CO2 pulse-labeling was successfully developed that is easily applicable in remote natural ecosystems.

Cited by 8 publications

References 47 publications

Decrease in heathland soil labile organic carbon under future atmospheric and climatic conditions

Decrease in heathland soil labile organic carbon under future atmospheric and climatic conditions

Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy

Isotopic methods for non‐destructive assessment of carbon dynamics in shrublands under long‐term climate change manipulation

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