In situ SERS monitoring of intracellular H₂O₂ in single living cells based on label-free bifunctional Fe₃O₄@Ag nanoparticles

Abstract

Visualization of signaling molecules in single living cells is crucial for understanding cellular metabolism and physiology, which can provide valuable insights for early diagnoses and treatments of diseases. Highly sensitive...

Competitive Ratiometric Aptasensing with Core-Internal Standard-Shell Structure Based on Surface-Enhanced Raman Scattering

“…Owing to large surface amine groups, PEI was used as a coupler to integrate FeMMs and APA via electrostatic interaction . Briefly, 0.04 g of FeMMs was weighed into 20 mL of DI water containing 0.1 g of PEI and then ultrasonically mixed for 2 h to obtain FeMMs@PEI and separated by magnetic force.…”

“…Owing to large surface amine groups, PEI was used as a coupler to integrate FeMMs and APA via electrostatic interaction. 27 Briefly, 0.04 g of FeMMs was weighed into 20 mL of DI water containing 0.1 g of PEI and then ultrasonically mixed for 2 h to obtain FeMMs@PEI and separated by magnetic force. The FeMMs@ PEI was redispersed in an aqueous solution (1 mg/mL) and mixed with 200 μL of APA with mechanical shaking for 3 h. The final FAPA was obtained by magnetic separation.…”

Parallel Monitoring of Glucose, Free Amino Acids, and Vitamin C in Fruits Using a High-Throughput Paper-Based Sensor Modified with Poly(carboxybetaine acrylamide)

“…H 2 O 2 molecules adsorb on Ch-Ag NP surfaces, generating hydroxyl radicals (•OH). Hydroxyl radicals reacted with colorless TMB, resulting in the formation of the TMB diamine oxidized in a blue form, as shown in Figure 4a [35,36]. Ch-Ag NPs exhibited excellent catalytic ability in the presence of H 2 O 2 , and the presence of chitosan increased the stability and storage of silver nanoparticles [37].…”

Recent Research Progress of Surface‐Enhanced Raman Scattering Dominated Analysis Strategies in Early Diagnosis of Diseases

“…SERS imaging, as an emerging imaging technology, has aroused tremendous interest of scientific researchers and medical personnel owing to the advantages of ultra-sensitive, high specificity, slight background interference, nondestructive test, and in situ visualization. [215][216][217][218][219][220][221][222][223] Zhong et al proposed a ratiometric SERS nanoprobe via intracellular matrix metalloproteinase-2 (MMP-2) in situ imaging for screening breast cancer cells. As described in Figure 13A, rhodamine B (Rh B)-coupled peptides and internal standard molecules 2-NT were co-modified on AuNPs to form this SERS nanoprobes.…”

“…The gradual maturity of SERS biological imaging approach and miniaturized Raman instruments has provided new assistance for SERS‐based disease diagnosis field. SERS imaging, as an emerging imaging technology, has aroused tremendous interest of scientific researchers and medical personnel owing to the advantages of ultra‐sensitive, high specificity, slight background interference, nondestructive test, and in situ visualization [215–223] . Zhong et al.…”