<i>In situ</i>net fishing of α-glucosidase inhibitors from evening primrose (<i>Oenothera biennis</i>) defatted seeds by combination of LC-MS/MS, molecular networking, affinity-based ultrafiltration, and molecular docking

Wang, Zhiqiang; Zhang, Yuxian; Yan, Hongyuan

doi:10.1039/d1fo03975j

Cited by 19 publications

(15 citation statements)

References 45 publications

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“…In the last three decades, especially high-calorie diets have been shown to affect lipid metabolism and lead to fat accumulation, resulting in obesity, which is a cause of various chronic diseases such as diabetes, hypertension, and cardiovascular disease ( 1 ). Many bioactive phytochemicals in natural plants have attracted the attention of researchers due to their hypoglycemic, lipid- lowering, and antioxidant properties ( 2 , 3 ). Screening the safe, effective, and conveniently extracted compounds from natural plants have become a new direction to managing diabetes and alleviating oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

Characterization, Hypoglycemic Activity, and Antioxidant Activity of Methanol Extracts From Amomum tsao-ko: in vitro and in vivo Studies

Xie

et al. 2022

Front. Nutr.

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The dried fruit of Amomum tsao-ko is well-known as a spice as well as a Chinese traditional herb. This study aimed to identify the bioactive constituents in the powder of methanol extract from Amomum tsao-ko (PMEAT) and to evaluate the hypoglycemic and antioxidant effects of PMEAT, in vitro and in vivo. We identified 36 phytochemicals in PMEAT by employing HPLC-MS/MS. PMEAT solution was found to have potent α-glucosidase-inhibiting activity (IC50, 0.145 mg/mL) in vitro, twice as strong as that of acarbose (IC50, 0.273 mg/mL). To investigate the hypoglycemic activity of PMEAT in vivo, we studied the impact of low-dose PMEAT (the addition of 100 mg/kg PMEAT to the mice diet) and high-dose PMEAT (200 mg/kg PMEAT addition) treatments in STZ-induced diabetic mice. After 6 weeks of intervention, significantly decreased fasting blood glucose (FBG) (p < 0.05), significantly decreased area under the curve (AUC) of the oral glucose tolerance test (p < 0.05), significantly decreased HOMA-IR (p < 0.05), and significantly increased HOMA-β (p < 0.05) were observed in the high-dose PMEAT group. Moreover, we performed an antioxidant activity experiment in vitro. The results showed that PMEAT had a strong ability to scavenge DPPH (IC50, 0.044 mg/mL) as well as ABTS free radicals (IC50, 0.040 mg/mL). In an animal experiment conducted on oxidative damage mice model which was induced by D-glucose and a high-fat diet, we observed significantly increased dismutase (SOD) (p < 0.01), glutathione (GSH) (p < 0.01), and glutathione peroxidase (GSH-Px) (p < 0.01) and significantly reduced malondialdehyde (MDA) and 8-ISO-prostaglandin-PGF2α (8-ISO-PGF2α), after treatment with PMEAT for 90 days. In conclusion, this study reveals the therapeutic potential of Amomum tsao-ko for the treatment of diabetes and helps us discover new antioxidant candidates from natural sources.

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Section: Introductionmentioning

confidence: 99%

Characterization, Hypoglycemic Activity, and Antioxidant Activity of Methanol Extracts From Amomum tsao-ko: in vitro and in vivo Studies

Xie

et al. 2022

Front. Nutr.

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“…In the past, the research was focused on investigating the health effects, such as the preventive effects on the body, but now, research has started to develop concerning the antioxidative side due to the important flavonoid content (e.g., flavonols, flavanols, anthocyanins, dihydrochalcones or phenolic acids) [ 26 , 27 , 28 , 29 , 30 ]. We can say that polyphenols are powerful antioxidants, and they can inhibit the reactive oxygen species’ formation as their scavengers [ 31 , 32 , 33 , 34 ]. They have good results as cosmetic active ingredients in cosmetic products, demonstrated by a high affinity for the reactive oxygen species (ROS), and can inhibit the formation of new ROS, by activating antioxidant enzymes, chelating pro-oxidant metal ions and neutralizing free radicals.…”

Section: Introductionmentioning

confidence: 99%

Efficient Extraction of Total Polyphenols from Apple and Investigation of Its SPF Properties

et al. 2022

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The purpose of this study was to evaluate the sun protection factor (SPF) of cosmetic emulsions with the addition of hydroalcoholic apple extract. First, the total polyphenolic content, the antioxidant activity and SPF properties of the extracts obtained by sonication and refluxing were evaluated. The two extraction methods were improved using the central composite design. For cosmetic emulsion that contained a different concentration of apple extract (10–40%), a SPF value between 0.51 and 0.90 was obtained. The most efficient apple extract was obtained by reflux using 50% ethanol and a 60 min extraction time. The concentrated extract was incorporated in a cosmetic emulsion whose SPF maximum was 0.90. Accordingly, due to photoprotective properties, the apple extract can be a candidate for use in cosmetic formulations.

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“…Owing to the complex chemical composition of natural product extracts and high structural diversity of their components, the identification of biologically active compounds using bioassay-guided isolation is laborious ( 13 ). Recently, affinity-based ultrafiltration hyphenated with liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been used for the in situ identification of bioactive compounds in natural product extracts ( 14 , 15 ). However, the detection of the minor bioactive compounds captured by proteins via affinity-based ultrafiltration using LC–MS/MS is challenging because the significant differences in extracts composition cause the signals of the major components to mask those of the minor components ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Tyrosinase Inhibitors in Dryopteris crassirhizoma Rhizome Using a Combination of High-Speed Counter-Current Chromatography, Affinity-Based Ultrafiltration, and Liquid Chromatography–Tandem Mass Spectrometry

Wang

Han

et al. 2022

Front. Nutr.

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Dryopteris crassirhizoma rhizome (DCR) inhibits melanin production in B16F10 melanoma cells and tyrosinase activity. The melanin content and tyrosinase activity of DCR-treated zebrafish embryos were determined to evaluate the in vivo inhibitory effect of DCR on melanogenesis. Moreover, an off-line hyphenated method combining the high-speed counter-current chromatography, affinity-based ultrafiltration, and liquid chromatography–tandem mass spectrometry was used to identify and characterize the DCR compounds with tyrosinase inhibitory activity. Our results indicated that DCR significantly decreased the melanin content and tyrosinase activity in zebrafish embryos in a dose-dependent manner; moreover, 22 compounds in DCR presented tyrosinase inhibitory activity. In silico molecular docking prediction data indicated that the 22 compounds in DCR can form stable conformations in the active site pocket of tyrosinase.

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In situnet fishing of α-glucosidase inhibitors from evening primrose (Oenothera biennis) defatted seeds by combination of LC-MS/MS, molecular networking, affinity-based ultrafiltration, and molecular docking

Abstract: Defatted seeds of evening primrose (DSEP), the by-product of evening primrose oil manufacture, exhibit potential α-glucosidase inhibitory activity; however, presently they are routinely discarded as waste. In this study, an...

Cited by 19 publications

References 45 publications

Characterization, Hypoglycemic Activity, and Antioxidant Activity of Methanol Extracts From Amomum tsao-ko: in vitro and in vivo Studies

Characterization, Hypoglycemic Activity, and Antioxidant Activity of Methanol Extracts From Amomum tsao-ko: in vitro and in vivo Studies

Efficient Extraction of Total Polyphenols from Apple and Investigation of Its SPF Properties

Characterization of Tyrosinase Inhibitors in Dryopteris crassirhizoma Rhizome Using a Combination of High-Speed Counter-Current Chromatography, Affinity-Based Ultrafiltration, and Liquid Chromatography–Tandem Mass Spectrometry

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