<i>In situ</i> fabrication of an anisotropic double-layer hydrogel as a bio-scaffold for repairing articular cartilage and subchondral bone injuries

Yu, Xiaotian; Deng, Zhantao; Li, Han; Ma, Yuanchen; Zheng, Qiujian

doi:10.1039/d3ra06222h

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The subchondral plate is a dense and crisscrossed poly-porous calcified plate. The subchondral bone trabeculae possess a spongy-like cancellous structure behind the subchondral plate, which contributes to continuous bone regeneration and remodeling and maintaining the physiological homeostasis microenvironment of the cartilage ( Su et al, 2023 ; Yu et al, 2023 ; Zhao et al, 2024 ) ( Figure 2B ).…”

Section: Bone Tissue Structure and Bone Tissue Microenvironmentmentioning

confidence: 99%

Smart responsive in situ hydrogel systems applied in bone tissue engineering

Wu,

Gai,

Chen

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The repair of irregular bone tissue suffers severe clinical problems due to the scarcity of an appropriate therapeutic carrier that can match dynamic and complex bone damage. Fortunately, stimuli-responsive in situ hydrogel systems that are triggered by a special microenvironment could be an ideal method of regenerating bone tissue because of the injectability, in situ gelatin, and spatiotemporally tunable drug release. Herein, we introduce the two main stimulus-response approaches, exogenous and endogenous, to forming in situ hydrogels in bone tissue engineering. First, we summarize specific and distinct responses to an extensive range of external stimuli (e.g., ultraviolet, near-infrared, ultrasound, etc.) to form in situ hydrogels created from biocompatible materials modified by various functional groups or hybrid functional nanoparticles. Furthermore, “smart” hydrogels, which respond to endogenous physiological or environmental stimuli (e.g., temperature, pH, enzyme, etc.), can achieve in situ gelation by one injection in vivo without additional intervention. Moreover, the mild chemistry response-mediated in situ hydrogel systems also offer fascinating prospects in bone tissue engineering, such as a Diels–Alder, Michael addition, thiol-Michael addition, and Schiff reactions, etc. The recent developments and challenges of various smart in situ hydrogels and their application to drug administration and bone tissue engineering are discussed in this review. It is anticipated that advanced strategies and innovative ideas of in situ hydrogels will be exploited in the clinical field and increase the quality of life for patients with bone damage.

show abstract

Section: Bone Tissue Structure and Bone Tissue Microenvironmentmentioning

confidence: 99%

Smart responsive in situ hydrogel systems applied in bone tissue engineering

Wu,

Gai,

Chen

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…These hydrogels not only fill cartilage defects but can also repair both cartilage and the underlying subchondral bone, 393 which supports mechanical strength and promotes cell migration and new tissue growth. 394 Moreover, since cartilage injuries often trigger inflammatory reactions that can hinder repair, designing hydrogels with anti-inflammatory properties is beneficial. For instance, a novel poly-anionic hydrogel enriched with carboxylate/sulfonate groups and closely cross-linked with Fe 3+ has shown effectiveness in suppressing hydroxyl radicals and nitric oxide in macrophages, protecting chondrocytes/fibroblasts from severe inflammation.…”

Section: Hydrogels For Cell Therapymentioning

confidence: 99%

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Lu,

Ruan,

Huang

et al. 2024

Sig Transduct Target Ther

View full text Add to dashboard Cite

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements and the potential of hydrogels in therapeutic applications, focusing primarily on two areas: emerging cell-based therapies and promising non-cell therapeutic modalities. Within the context of cell therapy, we discuss the capacity of hydrogels to overcome the existing translational challenges faced by mainstream cell therapy paradigms, provide a detailed discussion on the advantages and principal design considerations of hydrogels for boosting the efficacy of cell therapy, as well as list specific examples of their applications in different disease scenarios. We then explore the potential of hydrogels in drug delivery, physical intervention therapies, and other non-cell therapeutic areas (e.g., bioadhesives, artificial tissues, and biosensors), emphasizing their utility beyond mere delivery vehicles. Additionally, we complement our discussion on the latest progress and challenges in the clinical application of hydrogels and outline future research directions, particularly in terms of integration with advanced biomanufacturing technologies. This review aims to present a comprehensive view and critical insights into the design and selection of hydrogels for both cell therapy and non-cell therapies, tailored to meet the therapeutic requirements of diverse diseases and situations.

show abstract

Characterization of Acellular Cartilage Matrix-Sodium Alginate Scaffolds in Various Proportions

Lu,

Yang,

Zhang

et al. 2024

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

The development of three-dimensional (3D) bioprinting technology has provided a new solution to address the shortage of donors, multiple surgeries, and aesthetic concerns in microtia reconstruction surgery. The production of bioinks is the most critical aspect of 3D bioprinting. Acellular cartilage matrix (ACM) and sodium alginate (SA) are commonly used 3D bioprinting materials, and there have been reports of their combined use. However, there is a lack of comprehensive evaluations on ACM-SA scaffolds with different proportions. In this study, bioinks were prepared by mixing different proportions of decellularized rabbit ear cartilage powder and SA and then printed using 3D bioprinting technology and crosslinked with calcium ions to fabricate scaffolds. The physical properties, biocompatibility, and toxicity of ACM-SA scaffolds with different proportions were compared. The adhesion and proliferation of rabbit adipose-derived stem cells on ACM-SA scaffolds of different proportions, as well as the secretion of Collagen Type II, were evaluated under an adipose-derived stem cell chondrogenic induction medium. The following conclusions were drawn: when the proportion of SA in the ACM-SA scaffolds was <30%, the printed structure failed to form. The ACM-SA scaffolds in proportions from 1:9 to 6:4 showed no significant cytotoxicity, among which the 5:5 proportion of ACM-SA scaffold was superior in terms of adhesiveness and promoting cell proliferation and differentiation. Although a higher proportion of SA can provide greater mechanical strength, it also significantly increases the swelling ratio and reduces cell proliferation capabilities. Overall, the 5:5 proportion of ACM-SA scaffold demonstrated a more desirable biological and physical performance. Impact statement This study provides a more comprehensive in vitro assessment of material selection for bioinks in three-dimensional (3D) bioprinting technology for microtia reconstruction. By evaluating the biological performance and physical properties of acellular cartilage matrix-sodium alginate (ACM-SA) scaffolds in different proportions, we found that the 5:5 proportion of ACM-SA scaffold is more suitable as a constituent material for 3D bioprinting compared to other proportions. This provides meaningful foundational research data for our further in vivo animal experiments involving 3D bioprinted scaffolds loaded with cells.

show abstract

In situ fabrication of an anisotropic double-layer hydrogel as a bio-scaffold for repairing articular cartilage and subchondral bone injuries

Abstract: An anisotropic SA/DECM/agarose double-layer hydrogel is fabricated as a bio-scaffold for the repair of osteochondral injury.

Cited by 4 publications

References 27 publications

Smart responsive in situ hydrogel systems applied in bone tissue engineering

Smart responsive in situ hydrogel systems applied in bone tissue engineering

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Characterization of Acellular Cartilage Matrix-Sodium Alginate Scaffolds in Various Proportions

Contact Info

Product

Resources

About