In situ analysis of oxytetracycline tablets based on matrix‐assisted laser desorption/ionization mass spectrometry imaging

Abstract

Rationale:A thorough understanding of the content and distribution of active ingredients in pharmaceuticals is essential for drug efficacy and safety.Technological advancements in mass spectrometry imaging present an opportunity for methodological innovation by providing qualification and quantification analysis, as well as spatial information, in the same assay, which has great potential for applications in the rapid analysis and quality control of drugs.Methods: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was employed to directly analyze oxytetracycline tablets in order to map the distribution of the active constituent within the whole tablet. Quantitative analysis was capable of differentiating tablets containing various doses of the active pharmaceutical ingredient. Results:To establish the methodology, detailed factors that influence matrix spraying and spatial resolution during sample preparation and the data acquisition process were optimized systematically. Quantitative analysis could differentiate the tablets containing various doses of the active compound. The proposed method was successfully applied to analyze real commercial tablets. Conclusions:The developed method could successfully achieve the spatial location of oxytetracycline in actual tablet samples. These results could contribute to pharmaceutical tracing technology, especially the formulation process of tablets, which is helpful for monitoring the quality of pharmaceutical products and guaranteeing drug security.

Biomarkers of Mycoplasma pneumoniae pneumonia in children by urine metabolomics based on Q Exactive liquid chromatography/tandem mass spectrometry

“…Threonic acid is the substrate of L‐threonate 3‐dehydrogenase in the ascorbate and aldarate metabolism pathway and is a microbial metabolite 38 . It has been identified as a biomarker of cystic fibrosis 39 . Moreover, lung infection is the main cause of cystic fibrosis‐related morbidity and mortality 40 .…”

“…38 It has been identified as a biomarker of cystic fibrosis. 39 Moreover, lung infection is the main cause of cystic fibrosis-related morbidity and mortality. 40 finding further verified that threonic acid is biologically related to lung diseases.…”

Coupling of Laser Ablation and the Liquid Sampling-Atmospheric Pressure Glow Discharge Plasma for Simultaneous, Comprehensive Mapping: Elemental, Molecular, and Spatial Analysis

“…Fields such as pharmaceutical development, , nanotechnology, , and phytoremediation , have advanced significantly due to improvements in imaging methodologies. As an example, through mapping of nanoparticles in animal models, the complex mechanisms of drug–cell interactions can be elucidated .…”

“…Knowledge of both chemical and spatial information is becoming increasingly important as the location of specific elemental or molecular species within a sample can provide substantial insight into the function, or perhaps failure, of a system. 2 Fields such as pharmaceutical development, 3,4 nanotechnology, 5,6 and phytoremediation 7,8 have advanced significantly due to improvements in imaging methodologies. As an example, through mapping of nanoparticles in animal models, the complex mechanisms of drug−cell interactions can be elucidated.…”