<i>In silico</i> analysis of phytoene synthase and its promoter reveals hints for regulation mechanisms of carotenogenesis in <i>Duanliella bardawil</i>

Lao, Yong-Min; Xiao, Lan; Ye, Zhi‐Wei; Jiang, Jianguo; Zhou, Shishui

doi:10.1093/bioinformatics/btr371

Cited by 26 publications

(28 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the glycerol synthesis or degradation in D. salina treated by salt stress was due to the synergistic effect of four enzymes, which were propitious to the glycerol metabolism for osmotic adjustment of D. salina to respect to salt stress, and G3pdh play the key role in glycerol metabolism. In our previous study, it was found that several candidate motifs of the promoter of phytoene synthase (Psy), the first regulatory point in carotenogenesis in Dunaliella bardawil , exhibited salt-induced characteristics [27]. Psy is recognized as the rate-limiting enzyme in carotenoids synthesis.…”

Section: Resultsmentioning

confidence: 99%

Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses

Chen

Jiang

2012

PLoS ONE

View full text Add to dashboard Cite

The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses

Chen

Jiang

2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Previously, we isolated the promoter region of Dbpsy and found a cis-acting element candidate nominated as the GT-1 motif potentially involved in the hyperosmotic expression of psy (Lao et al, 2011). We believed that some vital CRTs responsible for b-carotene biosynthesis would be up-regulated under hyperosmolarity exerted by salt stress.…”

Section: Different Induction Characteristics Of Crts Might Correlate mentioning

confidence: 99%

“…Further knowledge on its regulation mechanisms for massive accumulation of b-carotene in response to salt stress remains to be discovered. Our previous study on the D. bardawil psy (Dbpsy) promoter region implied various regulatory elements involved in the transcriptional expression of the Dbpsy gene (Lao et al, 2011). It seems that the regulation of many key carotenogenic genes (crts) is partly attributed to related transcriptional regulatory elements residing in the promoter regions; therefore, in this study, we attempted to isolate the promoter and terminator of Dbzds and subsequently used them to express a fusion protein, fusion of Zeocin resistance gene (BLE) to the enhanced green fluorescent protein (EGFP), in D. bardawil.…”

mentioning

confidence: 99%

Hypoosmotic Expression of Dunaliella bardawil ζ-Carotene Desaturase Is Attributed to a Hypoosmolarity-Responsive Element Different from Other Key Carotenogenic Genes

Lao

Xiao

et al. 2014

Plant Physiology

View full text Add to dashboard Cite

Some key carotenogenic genes (crts) in Dunaliella bardawil are regulated in response to salt stress partly due to salt-inducible cisacting elements in their promoters. Thus, we isolated and compared the z-carotene desaturase (Dbzds) promoter with other crts promoters including phytoene synthase (Dbpsy), phytoene desaturase (Dbpds), and lycopene b-cyclase1 (DblycB1) to identify salt-inducible element(s) in the Dbzds promoter. In silico analysis of the Dbzds promoter found several potential cis-acting elements, such as abscisic acid response element-like sequence, myelocytomatosis oncogene1 recognition motif, AGC box, anaerobic motif2, and activation sequence factor1 binding site. Remarkably, instead of salt-inducible elements, we found a unique regulatory sequence architecture in the Dbzds promoter: a hypoosmolarity-responsive element (HRE) candidate followed by a potential hypoosmolarity-inducible factor GBF5 binding site. Deletion experiments demonstrated that only HRE, but not the GBF5 binding site, is responsible for hypoosmotic expression of the fusion of Zeocin resistance gene (ble) to the enhanced green fluorescent protein (egfp) chimeric gene under salt stress. Dbzds transcripts were in accordance with those of ble-egfp driven by the wild-type Dbzds promoter. Consequently, Dbzds is hypoosmotically regulated by its promoter, and HRE is responsible for this hypoosmotic response. Finally, the hypoosmolarity mechanism of Dbzds was studied by comparing transcript profiles and regulatory elements of Dbzds with those of Dbpsy, Dbpds, DblycB1, and DblycB2, revealing that different induction characteristics of crts may correlate with regulatory sequence architecture.Carotenoids are a structurally diverse class of isoprenoids synthesized by all photosynthetic organisms and many nonphotosynthetic organisms, such as certain species of bacteria, fungi, and archaea (Goodwin, 1980). They possess many advantageous properties for the human body on account of their vitamin A activity as essential nutrients (Farré et al., 2010), prevention and treatment functions against several kinds of diseases as health care products (Michaud et al., 2000;Landrum and Bone, 2001;Shaish et al., 2006), as well as industrial agents as colorants, forages, and cosmetics (SchmidtDannert, 2000). Therefore, the investigation of biosynthetic mechanisms and commercial exploitation of carotenoids have gained increasing attraction in many laboratories and companies. Recently, at least 700 carotenoids have been characterized from natural carotenoid biosynthetic pathways (Feltl et al., 2005).Some carotenogenic microorganisms have been commercially employed to produce important carotenoids (Johnson et al., 1995;Raja et al., 2007). Among these microorganisms, the Dunaliella genus, especially Dunaliella salina and Dunaliella bardawil, has been researched extensively and exploited as a natural source of carotenoids due to its striking ability to accumulate carotenoids under certain circumstances (Amotz et al., 1982), including high light intensity, high sa...

show abstract

“…In Duanliell bardawil and D. salina it was suggested that variation at the N-terminus may be accountable for fine tuning of PSY in elucidating the regulation mechanisms. However, the C-terminus of the PSY protein is special and contains the Trans-IPPS-H domain having aspartate rich region I, II (DELVD) and (DVGED) as conserved motifs as well as a substrate binding domain with a catalytic function (Lao et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

A novel phytoene synthase paralog from halophytic Salicorniaconfers salinity tolerance in plants

Rehman¹,

Khan²,

Ali³

2017

Turk J Bot

View full text Add to dashboard Cite

In silico analysis of phytoene synthase and its promoter reveals hints for regulation mechanisms of carotenogenesis in Duanliella bardawil

Abstract: jgjiang@scut.edu.cn.

Cited by 26 publications

References 47 publications

Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses

Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses

Hypoosmotic Expression of Dunaliella bardawil ζ-Carotene Desaturase Is Attributed to a Hypoosmolarity-Responsive Element Different from Other Key Carotenogenic Genes

A novel phytoene synthase paralog from halophytic Salicorniaconfers salinity tolerance in plants

Contact Info

Product

Resources

About