IL17Agene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection

Abstract

Interleukin (IL)-17A plays important roles in hepatitis B virus (HBV)-induced liver diseases. This study aims to investigate IL17A single nucleotide polymorphisms (SNPs) and the predispositions to chronic HBV infection and hepatocellular carcinoma (HCC) risk and the correlations to IL-17A and IgE levels. Three hundred ninety-five chronic HBV patients, 75 HBV infection resolvers, and 174 healthy controls were included. IL17A SNPs rs8193036 (C/T) and rs2275913 (A/G) and serum IL-17A and IgE levels were determined. HBV infection resolvers had higher rs8193036 allele T and allele T-containing genotypes than HBV patients or controls. Compared with chronic hepatitis, HCC patients had more frequent rs2275913 genotype GG (odds ratios [OR] 3.317, 95% confidence interval [CI] 1.663-6.617, P = 0.001) and allele G (OR 1.844, 95% CI 1.311-2.595, P < 0.001), and more frequent haplotypes CG (OR 1.868, 95% CI 1.256-2.778, P = 0.002) and TG (OR 1.788, 95% CI 1.031-3.101, P = 0.037) of rs8193036 and rs2275913. Comparison of HCC patients with cirrhosis yielded similar findings. Apart from male gender and older ages, IL-17A level (OR 1.020, 95% CI 1.003-1.036, P = 0.019) and rs2275913 genotypes AG and GG (OR 1.704, 95% CI 1.214-2.390, P = 0.006) were factors significantly associated with HCC risk in multivariate analysis in comparison with HBV patients without HCC. These factors remained significant in multivariate analysis in relation to cirrhosis. IL17A rs2275913 genotype GG was associated with significantly increased IL-17A and IgE levels. IL17A polymorphisms may influence HCC risk in chronic HBV infection via regulating IL-17A production.

Genetic Variants of Interleukin 17A Are Functionally Associated with Increased Risk of Age-Related Macular Degeneration

“…Some studies show that in vitro stimulated T cells from healthy individuals possessing the rs2275913A allele produced significantly more IL-17 than those without the A allele [5]. Other researchers reported that subjects carrying rs2275913GG genotype had higher serum level of IL-17 than those with AA genotype [12]. Using in vitro stimulated PBMCs, we identified that PBMCs with AA genotype excrete significantly higher level of IL-17 than those with GG genotype, and this phenomenon could be observed in both healthy controls (Fig.…”

Association between cytokine gene polymorphisms and outcome of hepatitis B virus infection in southern Brazil

“…Hardy–Weinberg equilibrium was tested for each locus using Markov chain as implemented in Arlequin software version 3.5 [Excoffier and Lischer, ]. Dominant and recessive genetic models were assumed based on previous studies of phenotype effects [Nakayama et al, ; Barrett et al, ; Christensen et al, ; Tayebi and Mohamadkhani, ; Li et al, ]. Haplotype frequencies were estimated using the MLOCUS computer program [Long et al, ].…”

Evaluation of IL‐17A, IL‐17F, IL‐23R, VDR, CCL2, CCL5, CCR2, and CCR5 gene polymorphisms and expression in Chinese individuals with syphilis

“…The results showed that IL-17A rs2275913 and rs3819024 polymorphisms were correlated with T. pallidum susceptibility to infection. 23,24 Interestingly, another study showed that IL-17A rs2275913 genotypes did not influence the expression levels of IL-17A in serum in patients with colorectal cancer. Therefore, the current study further analyzed haplotype containing rs2275913 and rs3819024 of IL-17 using SHEsis software.…”