Hypericum perforatum Oil and Vitamin A Palmitate-Loaded Gelatin Nanofibers Cross-Linked by Tannic Acid as Wound Dressings

Abstract

Recent studies in wound dressing applications offer new therapies to promote the wound healing process. The main strategy of this study is to combine the traditional perspective of using medicinal oils with polymeric scaffolds manufactured by an engineering approach to fabricate a potential tissue engineering product that provides both new tissue formation and wound healing. Thus, Hypericum perforatum oil (HPO) and vitamin A palmitate (VAP) incorporated gelatin (Gt) nanofibrous scaffolds were successfully prepared by the electrospinning method. Tannic acid (TA) was used as the cross-linking agent. The amounts of VAP and HPO loaded in the base Gt solution [15% w/v in 4:6 v/v acetic acid/deionized water] were 5 and 50 wt % (based on the weight of Gt), respectively. The obtained scaffolds were studied regarding their microstructure, chemical structure, thermal stability, antibacterial activity, in vitro release study, and cellular proliferation assay. In the light of these studies, it was determined that VAP and HPO were incorporated successfully in Gt nanofibers cross-linked with TA. Release kinetic tests confirmed that the patterns of TA and VAP release were consistent with the Higuchi model, whereas HPO release was consistent with the first-order kinetic model. In addition, this membrane was biocompatible with L929 fibroblast cells and had antibacterial activity and thermal stability. This preliminary study suggests potential applicability of the proposed dressing to treat skin wounds in clinics.

Fabrication of a Biocompatible Nanoantimicrobial Suture for Rapid Wound Healing after Surgery

“…St John's wort, Hypericum perforatum, is known as a medicinal plant which has been used in popular medicine and phytotherapy for its well-documented antiseptic effects. 6 It has been traditionally used as a folk remedy for the management of wounds, bruises, skin ulcers, cuts, burns, contusions, depression, and myalgia. 7 There are several antiseptic-coated sutures, such as triclosan and chlorhexidine, that have been commercially available for medical or veterinary use.…”

“…St John’s wort, Hypericum perforatum, is known as a medicinal plant which has been used in popular medicine and phytotherapy for its well-documented antiseptic effects . It has been traditionally used as a folk remedy for the management of wounds, bruises, skin ulcers, cuts, burns, contusions, depression, and myalgia …”

Highly Compressible, Superabsorbent, and Biocompatible Hybrid Cryogel Constructs Comprising Functionalized Chitosan and St. John’s Wort Extract

“…A large portfolio of bioactive compounds has been identified in the composition of HYP, including phloroglucinol derivatives (hyperforin, adhyperforin), naphthodianthrones (hypericin and pseudohypericin), and flavonoids (rutin, hyperoside, isoquercitrin, quercitrin, quercetin). ,,,, However, despite their promising biological activities, these compounds show low water solubility, inadequate bioavailability, and poor chemical stability under various environmental conditions like light, air, or heat, , which restrict their potential applications in value-added products. To overcome these drawbacks, and also to improve their biological activity, their encapsulation in different multifunctional systems, including lipid nanocapsules, thermoresponsive liposomes, poly(lactic- co -glycolic acid) nanoparticles, or hydrogels, − has been pursued.…”

“…In ancient times, HYP was not only thought to have healing properties but also magical powers . Traditional uses of HYP include a plethora of indications, both externally (wound healing and antiseptic uses) − and internally (for digestive ailments such as diarrhea and gastric ulcer, or as a sedative in psychiatric disorders). − …”