<i>Hsr-omega</i>, A Novel Gene Encoded by a <i>Drosophila</i> Heat Shock Puff

Pardue, Mary Lou; Bendena, William G.; Fini, M E; Garbe, James C.; Hogan, N. Catherine; Traverse, Karen L.

doi:10.2307/1541741

Cited by 28 publications

(23 citation statements)

References 23 publications

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“…While the molecular and expression patterns of one of the classic heat shock genes of Drosophila, hsr-omega, have been well described (Pardue et al 1990), the significance of this gene to the cell and for the whole organism are not well understood. A growing body of data suggest that it may influence both protein synthesis (summarised in Lakhotia 2003) and heat tolerance (McKechnie et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…According to Pardue et al (1990), the polysomal-associated turnover of omega-c may serve to monitor or regulate some aspect of protein synthesis in the cell. The second, nuclearrestricted transcript, omega-n, may also influence protein synthesis by controlling release into the cytoplasm of messenger RNAs-the essential templates for building proteins.…”

Section: Introductionmentioning

confidence: 99%

“…At such a location genotype and phenotype variation is likely to be maximised, and the use of singlepair lines both limits variation within lines and maximises variation among lines. Associations between protein synthesis capacity, measured in ovarian tissue (both before and Pardue et al 1990). Positions of omega-n and omega-c transcript-specific primers used for qRT-PCR are indicated by arrows after a mild heat shock) and variation in both hsr-omega transcript levels and genotype are explored.…”

Section: Introductionmentioning

confidence: 99%

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Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Johnson

Carrington

Hallas

et al. 2009

Cell Stress and Chaperones

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Transcripts of the Drosophila hsr-omega gene are known to interact with RNA processing factors and ribosomes and are postulated to aid in co-ordinating nuclear and cytoplasmic activities particularly in stressed cells. However, the significance of these interactions for physiological processes and in turn for whole-organism fitness remains an open question. Because hsr-omega's cellular expression characteristics suggest it may influence protein synthesis, and because both genotypic and expression variation of hsr-omega have been associated with thermotolerance, we characterised 30 lines for variation in the rates of protein synthesis, measured in ovarian tissues, both before and after a mild heat shock, and for basal levels of the two main hsr-omega transcripts, omega-n and omega-c. As expected, the mild heat shock reduced protein synthesis rates. Large variation occurred among lines in levels of omega-n which was negatively associated with rates of basal protein synthesis-a result that supports the model for the cellular function of omega-n. Furthermore, omega-n levels were associated with hsr-omega genotype of the line parents. Little variation occurred among lines for omega-c levels and no associations were detected with protein synthesis or genotype. Since protein synthesis is a fundamental process for growth and development, we characterised the lines for several life-history traits; however, no associations with protein synthesis, omega-n or omega-c levels were detected. Our results are consistent with the idea that natural variation in hsr-omega expression influence rates of protein synthesis in this species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Johnson

Carrington

Hallas

et al. 2009

Cell Stress and Chaperones

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“…In Drosophila melanogaster one candidate gene that has been subject to thorough investigation at the molecular and cellular level is hsr-omega (Pardue et al, 1990). This gene does not produce a protein product.…”

Section: Introductionmentioning

confidence: 99%

“…This gene does not produce a protein product. It produces two main RNA transcripts both of which are constitutively expressed in most adult tissue types, one located in the cytoplasm, omega-c, and one in the nucleus, omega-n. hsr-Omega has long been recognized as one of the heatstress genes as it is upregulated by a mild heat shock (similar to the heat-shock protein genes), and because overlapping deletion mutants of hsr-omega are thermosensitive (Pardue et al, 1990). Also, hsr-omega shares with the other heat-shock genes isolated from natural populations an abundance of P-element inserts in the promoter region (Walser et al, 2006)-inserts that may influence heat-shock gene expression and thermal adaptive potential (Chen et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Latitudinal and cold-tolerance variation associate with DNA repeat-number variation in the hsr-omega RNA gene of Drosophila melanogaster

et al. 2008

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Spatial expression of the hsr‐omega (93D) gene in different tissues of drosophila melanogaster and identification of promoter elements controlling its developmental expression

Mutsuddi

Lakhotia

1995

Dev. Genet.

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Developmental expression of the heat shock inducible non-protein coding hsromega gene in several iarval and adult tissues of Drosophila melanogaster was examined by in situ hybridization to transcripts in intact organs and by X-gal staining in the germline transformants carrying the lacZ reporter gene under the control of hsromega promoter. This gene is expressed in a specific spatial pattern in all the larval and adult tissue types examined; however, its transcripts were specifically absent in certain gonadal cell types like the male as well as female gonial cells and in follicle cells and oocytes in ovary. All polytenised tissues like the prothoracic and salivary glands, certain regions of larval gut and the Malpighian tubules showed a greater abundance of hsr-omega transcripts with a strong hybridization in nuclei. Our results with promoter deletion variant germline transformants suggest that a region between -346bp to -844bp upstream contains major regulatory element/s for developmental expression of this gene in most of the larval and adult tissues examined; however, this region is not sufficient for its normal expression in male and female reproductive systems. An analysis of the base sequence of the hsr-omega promoter (upto -844bp) reveals putative ecdysone receptor element half-sites and two GAGA factor binding sites which may be involved in its developmental expression and its ready inducibility. The widespread expression in most tissue types and the known lethality associated with its homozygous deletion, suggest that the variety of non-protein coding transcripts of the hsromega gene have vital "house-keeping" functions. 0 1995 Wiley-Liss, Inc.

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Hsr-omega, A Novel Gene Encoded by a Drosophila Heat Shock Puff

Cited by 28 publications

References 23 publications

Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Protein synthesis rates in Drosophila associate with levels of the hsr-omega nuclear transcript

Latitudinal and cold-tolerance variation associate with DNA repeat-number variation in the hsr-omega RNA gene of Drosophila melanogaster

Spatial expression of the hsr‐omega (93D) gene in different tissues of drosophila melanogaster and identification of promoter elements controlling its developmental expression

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