<i>Hsd3b2</i> associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells

Zhang, Chen; Wang, Man; He, Na; Ahmed, Mahmoud F.; Wang, Yilin; Zhao, Ruihua; Yu, Xudong; Jin, Jing; Song, Jiuzhou; Zuo, Qisheng; Zhang, Yani

doi:10.1002/jcb.26279

Cited by 6 publications

(2 citation statements)

References 27 publications

(56 reference statements)

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“…Liu et al [ 32 ] performed RNA-seq analysis of goat ovaries in the follicular and luteal phases and found HSD17B7 to be a differentially expressed gene, implying that HSD17B7 is a gene that regulates ovarian development in goats. HSD17B7 is also involved in regulating the steroid hormone synthesis pathway and the differentiation of chicken embryonic stem cells into spermatogonial stem cells [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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Litter size is an economically important trait in sheep, and it is a complex trait controlled by multiple genes in multiple organs. Among them, the regulation of lamb number trait by the thyroid gland is a very important part. However, the molecular mechanisms of the thyroid gland in sheep reproduction remain unclear. Here, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) STH sheep, respectively, and to identify differentially expressed circRNAs (DECs) associated with reproduction. Bioinformatic analysis of the source genes of these DECs revealed that they can be enriched in multiple signaling pathways involved in the reproductive process of animals. We found that the source genes of these DECs, such as GNAQ, VEGFC, MAPK1, STAT1, and HSD17B7, may play important roles in the reproductive process of animals. To better understand the function of these DECs, we constructed circRNA–miRNA co-expression networks. Dual luciferase reporter assays suggested that a ceRNA regulatory mechanism between circ_0003259-oar-miR-133-TXLNA and circ_0012128-oar-miR-370-3p-FGFR1 may hold. All of these DEC expression profiles in the thyroid gland provide a novel resource for elucidating the regulatory mechanisms underlying STH sheep prolificacy.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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“…Hatching conditions were 37.5°C and 65% relative humidity. For speci c separation and cultivation, refer to Zhang's article (Zhang, Wang et al 2017).…”

Section: Isolation and Culture Of Escs Pgcs And Sscsmentioning

confidence: 99%

Long Noncoding RNA LncPGCR mediated by TCF7L2 regulates chicken PGCs formation

Jiang

Zuo

Cheng

et al. 2020

Preprint

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Background: Several of the thousands of long noncoding RNAs (lncRNAs) have been functionally characterized, yet its specific function and molecular mechanism in the formation of chicken primordial germ cells (PGCs) remains poorly understood. In the present study, we aim to investigate the role of LncPGCR (LncRNA PGCs Regulator) in PGCs formation.Methods: LncPGCR, Cvh, Nanog, C-kit, gga-mir-6577-5p and Btrc expressions were detected by qRT-PCR. The percentage of PGCs cells was detected by flow cytometry, immunocytochemistry, and PAS staining. The interaction of histone acetylation, DNA methylation, transcription factor TCF7L2, and LncPGCR was confirmed by Luciferase reporter assay. The interaction of GAPDH and LncPGCR was measured by RNA pull-down, RIP, and Western blot.Results: We observed the increased expression of LncPGCR in PGCs. It is mainly expressed in the cytoplasm and encodes small peptides. Moreover, over-expression of LncPGCR could promote PGCs formation in vitro and in vivo. Besides, we first reported that histone acetylation, DNA methylation, and transcription factor TCF7L2 can regulate LncPGCR expression in PGCs. In addition, LncPGCR activates WNT signaling pathways to promote PGCs formation by adsorbing gga-mir-6577-5p, relieving its inhibitory effect on target gene Btrc. Meanwhile, LncPGCR contributed to PGCs formation by increasing the phosphorylation level of GAPDH to activate the TGF-β signal pathway.Conclusion: LncPGCR over-expression promoted ESCs differentiation into PGCs through the potential LncPGCR/miR-6577-5p/BTRC pathway or increasing the phosphorylation level of GAPDH to activate the TGF-β signal pathway.

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Testicular tissue engineering

Bhaskar

Gupta

2020

Biopolymer-Based Formulations

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Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells

Cited by 6 publications

References 27 publications

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Long Noncoding RNA LncPGCR mediated by TCF7L2 regulates chicken PGCs formation

Testicular tissue engineering

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