<i>Hox</i>Gene Misexpression and Cell-Specific Lesions Reveal Functionality of Homeotically Transformed Neurons

Hale, Melina E.; Kheirbek, Mazen A.; Schriefer, Julie E.; Prince, Victoria

doi:10.1523/jneurosci.5624-03.2004

Cited by 35 publications

(31 citation statements)

References 24 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of hoxb1 mRNA-injected animals were morphologically normal and typically had two Mauthner axons on one side of the CNS and one on the contralateral side. A subset of hoxb1 mRNA-injected animals had morphological abnormalities as have been previously described (Hale et al, 2004), and were therefore excluded from further study.…”

Section: Hoxb1 Mrna Injectionmentioning

confidence: 99%

“…In order to have an independent method to generate extra Mauthner axons we injected embryos with hoxb1 mRNAs (Fig. 3C), which has previously been shown to be capable of generating animals with ectopic Mauthner neurons by duplicating rhombomere 4 identity in the hindbrain (Hale et al, 2004). Examination of notch1a morphants and embryos injected with hoxb1 mRNA between 3 and 9 dpf using transgenic reporters (sox10:mRFP and mbp:EGFP-CAAX) and transmission electron microscopy showed that normal and supernumerary Mauthner axons were covered by glial membrane and robustly myelinated ( Fig.…”

Section: Supernumerary Mauthner Axons Are Robustly Myelinated In Vivomentioning

confidence: 99%

See 1 more Smart Citation

Individual axons regulate the myelinating potential of single oligodendrocytes in vivo

et al. 2011

View full text Add to dashboard Cite

SUMMARYThe majority of axons in the central nervous system (CNS) are eventually myelinated by oligodendrocytes, but whether the timing and extent of myelination in vivo reflect intrinsic properties of oligodendrocytes, or are regulated by axons, remains undetermined. Here, we use zebrafish to study CNS myelination at single-cell resolution in vivo. We show that the large caliber Mauthner axon is the first to be myelinated (shortly before axons of smaller caliber) and that the presence of supernumerary large caliber Mauthner axons can profoundly affect myelination by single oligodendrocytes. Oligodendrocytes that typically myelinate just one Mauthner axon in wild type can myelinate multiple supernumerary Mauthner axons. Furthermore, oligodendrocytes that exclusively myelinate numerous smaller caliber axons in wild type can readily myelinate small caliber axons in addition to the much larger caliber supernumerary Mauthner axons. These data indicate that single oligodendrocytes can myelinate diverse axons and that their myelinating potential is actively regulated by individual axons.

show abstract

Section: Hoxb1 Mrna Injectionmentioning

confidence: 99%

Section: Supernumerary Mauthner Axons Are Robustly Myelinated In Vivomentioning

confidence: 99%

Individual axons regulate the myelinating potential of single oligodendrocytes in vivo

et al. 2011

View full text Add to dashboard Cite

show abstract

“…One compelling example of this approach was to inject a Hox gene, which leads to a conversion of a neuron that does not normally form a Mauthner cell into an extra Mauthner neuron (Hale et al, 2004). The result is fish with two Mauthner cells, one in the normal hindbrain segment #4 and the other in hindbrain segment #2.…”

Section: Mutants and Genetic Perturbationsmentioning

confidence: 99%

Zebrafish and motor control over the last decade

Fetcho¹,

Higashijima²,

McLean³

2008

Brain Research Reviews

113

View full text Add to dashboard Cite

The combination of transparency and accessible genetics is making zebrafish an increasingly important model in studies of motor control. Much of the work on the model has been done over the past decade. Here we review some of the highlights of this work that serve to reveal both the power of the model and its prospects for providing important future insights into the links between neural networks and behavior.

show abstract

“…Eventually, the arrangement of dorsal fin motoneurons has to be revisited in future studies when reliable anatomical markers for segment borders are available. Candidates are Hox genes (Hale et al, 2004;MorinKensicki, 2002), genes of the notch pathway (Holley et al, 2002;Prince et al, 2001), and LIM/homeobox genes (Tokumoto et al, 1995;Toyama et al, 1995). Considering the fact that dorsal fin motoneurons are organized only in a few segments of the spinal cord, paraxial mesodermderived signals that appear to participate in the development of distinctive identities of primary motoneurons (Lewis and Eisen, 2004) might participate in the development of dorsal fin motoneurons.…”

Section: Comparison Of Dorsal and Caudal Fin Motoneuronsmentioning

confidence: 99%

Innervation of dorsal and caudal fin muscles in adult zebrafishDanio rerio

Schneider

Sulner

2006

J. Comp. Neurol.

View full text Add to dashboard Cite

The organization of the neuromuscular system of the dorsal and caudal fin of zebrafish, Danio rerio, was studied, including the anatomy of fin motoneurons as revealed by neurobiotin backfills and differential staining using fluorescent markers. The musculature of the dorsal fin consists of one pair of protractor and retractor muscles and 10 sets of muscles attaching to the bases of dorsal fin rays. Each set consists of one pair of erector, depressor, and inclinator muscles. The supplying nerves of the dorsal fin musculature originate from spinal segments 9-17 and form a dorsal fin plexus at the base of the muscles. Dorsal and caudal fin motoneurons have small cell bodies and ipsilateral dendritic branching patterns, thus resembling secondary motoneurons of the axial musculature. As shown by differential staining using fluorescent-labeled dextrans, cell bodies of dorsal fin motoneurons and axial motoneurons seem to be located in separate motor columns. The musculature of the caudal fin is composed of 12 muscles that are arranged in a superficial and a deep muscle layer. The nerves that supply the caudal fin musculature arise from the last five caudal segments of the spinal cord and form the caudal plexus. Neurobiotin backfills were performed on the dorsal caudal muscles, the medial caudal muscles, and the ventral caudal muscles. Most cell bodies of caudal fin motoneurons are small and are located in a ventral motor column. The organization of dorsal and caudal fin motoneurons is compared with the innervation of fins in other fish.

show abstract

HoxGene Misexpression and Cell-Specific Lesions Reveal Functionality of Homeotically Transformed Neurons

Cited by 35 publications

References 24 publications

Individual axons regulate the myelinating potential of single oligodendrocytes in vivo

Individual axons regulate the myelinating potential of single oligodendrocytes in vivo

Zebrafish and motor control over the last decade

Innervation of dorsal and caudal fin muscles in adult zebrafishDanio rerio

Contact Info

Product

Resources

About