Hibiscus SabdariffaLinnaeus Aqueous Extracts Attenuate the Progression of Renal Injury in 5/6 Nephrectomy Rats

Abstract

Hibiscus sabdariffa Linn. (HS) is a tropical wild plant with antioxidant, antibacterial, antihypertensive, and lipid-lowering properties. In several animal models, HS aqueous extracts reduced the severity of the multi-organ injuries such as hypertension and diabetic nephropathy. One of the multiorgan injuries is chronic kidney disease (CKD), which results from the loss of nephron function. HS was used in a 5/6 nephrectomy (5/6 Nx) rat model to determine if it could attenuate the progression of CKD. HS (250 mg/kg/day) or placebo was orally administered to 5/6 Nx male Sprague-Dawley rats. The NxþHS group had fewer renal injuries as measured by blood urea nitrogen, serum creatinine, creatinine clearance, and renal pathology when compared with the Nx group. In order to determine which property of HS, either vasodilatory and/or antioxidant, was important in attenuating the progression of CKD, systolic blood pressure (SBP) and serum levels of malondialdehyde (MDA) were assessed. In the NxþHS group, the SBP and the serum levels of MDA were significantly lower at Week 7. In conclusion, through either antihypertensive and/or antioxidant properties, HS was able to attenuate the progression of renal injury after 5/6 Nx. Hence, HS should be considered as one of the new, promising drugs that can be used to attenuate the progression of CKD.

Cilostazol attenuates intimal hyperplasia in a mouse model of chronic kidney disease

“…A tail cuff plethysmography (IITC Life Scientific Instruments, Woodland Hills, CA, USA) was used for the measurement of systolic blood pressure (SBP) as previously described elsewhere [ 25 ]. SBP was measured before the clamping of left renal bundle at wk-1 and then again at 4, 12, and 20 weeks after right nephrectomy.…”

Effect of aqueous extract and anthocyanins of calyces of Hibiscus sabdariffa (Malvaceae) in rats with adenine-induced chronic kidney disease

“…The aqueous extract of HS was prepared as described earlier . Fifty and 100 g of HS were placed separately in 950 and 900 ml distilled water, respectively, shaken for 5 min, left overnight at room temperature and filtered to provide the 5 and 10% aqueous extracts.…”

“…Hibiscus sabdariffa extract has been shown previously to be effective in a surgical model of CKD, [20] but the constituents of the extract responsible for this activity were not determined. The most active constituents of HS extracts have been shown on several occasions to be anthocyanins (ref.…”

Mechanisms of Herbal Nephroprotection in diabetes mellitus

“…), reactive nitrogen species (tocotrienol [50], etc. ), and lipid peroxidation products (breviscapine [51,52], Flammulina velutipes [53], Hibiscus sabdariffa [54], and many more-see Table 2). Beside decreasing malondialdehyde, Artemisia campestris also lowered the level of nitric oxide (NO) and advanced oxidation protein products [55].…”