<i>hgf/c‐met</i> expression and functional analysis during zebrafish embryogenesis

Latimer, Andrew J.; Jessen, Jason R.

doi:10.1002/dvdy.21794

Cited by 21 publications

(33 citation statements)

References 67 publications

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“…The results indicated that a number of MET expression-related genes, including mmp2 (26), mif (27), ybx1 (28), ctnnb1 (29), sub1 (30), rac1 (31), rps6kb1 (32), fn1 (33), and cdh2 (34), were down-regulated in the context of GrnA deficiency. MET, which encodes a receptor tyrosine kinase, is known to be critical for liver size in mice (5) and zebrafish (35). The suppressed expression of MET-related genes was further confirmed using quantitative RT-PCR in 72-hpf grnA morphants (Fig.…”

Section: Liver Volumementioning

confidence: 61%

“…To elucidate the molecular mechanisms involved in GrnAregulated liver growth, we performed a cDNA microarray analysis, which showed that GrnA modulated the expression of met and other related genes. During embryogenesis, Met and its ligand, the scatter factor/hepatocyte growth factor, play crucial roles in regulating liver development (49), nerve outgrowth (50), and myoblast migration from somites to the limbs (5,35). In addition, dysregulation of the MET signaling pathway promotes invasive growth and initiates metastasis during tumorigenesis (51,52).…”

Section: Discussionmentioning

confidence: 99%

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Progranulin A-mediated MET Signaling Is Essential for Liver Morphogenesis in Zebrafish

Chen

Gong

et al. 2010

Journal of Biological Chemistry

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The mechanism that regulates embryonic liver morphogenesis remains elusive. Progranulin (PGRN) is postulated to play a critical role in regulating pathological liver growth. Nevertheless, the exact regulatory mechanism of PGRN in relation to its functional role in embryonic liver development remains to be elucidated. In our study, the knockdown of progranulin A (GrnA), an orthologue of mammalian PGRN, using antisense morpholinos resulted in impaired liver morphogenesis in zebrafish (Danio rerio). The vital role of GrnA in hepatic outgrowth and not in liver bud formation was further confirmed using whole-mount in situ hybridization markers. In addition, a GrnA deficiency was also found to be associated with the deregulation of MET-related genes in the neonatal liver using a microarray analysis. In contrast, the decrease in liver size that was observed in grnA morphants was avoided when ectopic MET expression was produced by co-injecting met mRNA and grnA morpholinos. This phenomenon suggests that GrnA might play a role in liver growth regulation via MET signaling. Furthermore, our study has shown that GrnA positively modulates hepatic MET expression both in vivo and in vitro. Therefore, our data have indicated that GrnA plays a vital role in embryonic liver morphogenesis in zebrafish. As a result, a novel link between PGRN and MET signaling is proposed.

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Section: Liver Volumementioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Progranulin A-mediated MET Signaling Is Essential for Liver Morphogenesis in Zebrafish

Chen

Gong

et al. 2010

Journal of Biological Chemistry

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“…One particularly important role of vascular endothelial cells during liver differentiation is to provide signals required to polarize adjacent hepatocytes, as well as to promote biliary development (162). Recent studies of liver growth in zebrafish have shown that it is regulated by signaling pathways known to play a role in mammalian liver development, such as hepatocyte growth factor (HGF)-c-Met pathway (102). Novel factors affecting liver growth have also been reported (10,12).…”

Section: Liver Development In Zebrafishmentioning

confidence: 99%

Zebrafish Models of Human Liver Development and Disease

Wilkins

Pack

2013

Comprehensive Physiology

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The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their disruption underlies a diverse group of heritable and acquired diseases that affect both pediatric and adult patients. Although experimental analyses used to study liver development and disease are typically performed in cell culture models or rodents, the zebrafish is increasingly used to complement discoveries made in these systems. Forward and reverse genetic analyses over the past two decades have shown that the molecular program for liver development is largely conserved between zebrafish and mammals, and that the zebrafish can be used to model heritable human liver disorders. Recent work has demonstrated that zebrafish can also be used to study the mechanistic basis of acquired liver diseases. Here, we provide a comprehensive summary of how the zebrafish has contributed to our understanding of human liver development and disease.

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“…The met proto-oncogene is active in normal embryogenesis (230). It acts as an oncogene in the N-methyl-N'-nitro-N-nitrosoguanidine-(MNNG)-transformed human osteosarcoma (HOS) cell line (231)(232)(233).…”

Section: Met Mc/msh-r Mitfmentioning

confidence: 99%

Horizontal gene transfers and cell fusions in microbiology, immunology and oncology (Review)

Sinkovics¹

2009

Int J Oncol

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hgf/c‐met expression and functional analysis during zebrafish embryogenesis

Cited by 21 publications

References 67 publications

Progranulin A-mediated MET Signaling Is Essential for Liver Morphogenesis in Zebrafish

Progranulin A-mediated MET Signaling Is Essential for Liver Morphogenesis in Zebrafish

Zebrafish Models of Human Liver Development and Disease

Horizontal gene transfers and cell fusions in microbiology, immunology and oncology (Review)

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