<i>HFM1</i>, the human homologue of yeast Mer3, encodes a putative DNA helicase expressed specifically in germ-line cells

Tanaka, Kazunori; Miyamoto, Natsuki; Shouguchi-Miyata, Junko; Ikeda, Joh‐E

doi:10.1080/10425170600805433

Cited by 38 publications

(30 citation statements)

References 15 publications

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“…The mer3 mutant reduces the frequency of crossovers, and the remaining crossovers are distributed randomly along the chromosomes. Recently, homologs of MER3 were identified from Arabidopsis (Mercier et al 2005), humans (Tanaka et al 2006), Coprinus cinereus (Sugawara et al 2009) and rice (Wang et al 2009). In Arabidopsis, the Atmer3 mutation leads to defective crossover formation (Mercier et al 2005).…”

Section: Discussionmentioning

confidence: 96%

OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice

Wang

Tang

et al. 2010

Chromosoma

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Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination. In the present study, we silenced the SPO11-1 gene in rice (Oryza sativa) using RNAi. Rice plants with loss-of-function of OsSPO11-1 have no apparent growth defects during vegetative development, but homologous chromosome pairing and recombination are significantly obstructed. Telomeres can be assembled as bouquet during the zygotene stage of the OsSPO11-1-deficient plants, just as that in wild type. Although the two axial-associated proteins, REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type. The central element of the synaptonemal complex (SC), ZEP1, does not load onto the chromosomes normally, implying that SC formation is disturbed severely. The crossover protein, MER3, isn't efficiently assembled onto chromosomes and the lack of bivalent suggests that crossovers are also affected in the absence of OsSPO11-1. Thus, OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.

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Section: Discussionmentioning

confidence: 96%

OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice

Wang

Tang

et al. 2010

Chromosoma

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“…Of note, the maximal fold change in GBC versus nonneoplastic gallbladder was observed for the SAGE tag corresponding to the human homologue of the yeast MER3 gene (hHFM1), a RecQ-like helicase involved in meiosis (22). The gene product of HFM1 is an evolutionarily conserved helicase predominantly expressed in the gonads, and it is postulated to play a role in the maintenance of genomic stability during meiosis (23). To the best of our knowledge, this is the first demonstrable association between hHFM1 and human cancer.…”

Section: Resultsmentioning

confidence: 99%

Serial Analysis of Gene Expression Identifies Connective Tissue Growth Factor Expression as a Prognostic Biomarker in Gallbladder Cancer

Alvarez¹,

Corvalán²,

Roa

et al. 2008

Clinical Cancer Research

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Background: Gallbladder cancer (GBC) is an uncommon neoplasm in the United States, but one with high mortality rates.This malignancy remains largely understudied at the molecular level such that few targeted therapies or predictive biomarkers exist. Experimental Design: We built the first series of serial analysis of gene expression (SAGE) libraries from GBC and nonneoplastic gallbladder mucosa, composed of 21-bp long-SAGE tags. SAGE libraries were generated from three stage-matched GBC patients (representing Hispanic/ Latino, Native American, and Caucasian ethnicities, respectively) and one histologically alithiasic gallbladder. Real-time quantitative PCR was done on microdissected epithelium from five matched GBC and corresponding nonneoplastic gallbladder mucosa. Immunohistochemical analysis was done on a panel of 182 archival GBC in high-throughput tissue microarray format. Results: SAGE tags corresponding to connective tissue growth factor (CTGF) transcripts were identified as differentially overexpressed in all pairwise comparisons of GBC (P < 0.001). Realtime quantitative PCR confirmed significant overexpression of CTGF transcripts in microdissected primary GBC (P < 0.05), but not in metastatic GBC, compared with nonneoplastic gallbladder epithelium. By immunohistochemistry, 66 of 182 (36%) GBC had high CTGF antigen labeling, which was significantly associated with better survival on univariate analysis (P = 0.0069, log-rank test). Conclusions: An unbiased analysis of the GBC transcriptome by SAGE has identified CTGF expression as a predictive biomarker of favorable prognosis in this malignancy. The SAGE libraries from GBC and nonneoplastic gallbladder mucosa are publicly available at the Cancer Genome Anatomy Project web site and should facilitate much needed research into this lethal neoplasm.

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“…Recently, homologs of MER3 were identified in Arabidopsis (Mercier et al 2005;Chen et al 2005) and humans (Tanaka et al 2006). In Arabidopsis, T-DNA insertional mutants of the gene are defective in homolog synapsis and crossover formation, resulting in a reduction of bivalent formation and increase in the number of univalents at late prophase I.…”

Section: Introductionmentioning

confidence: 96%

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

et al. 2008

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Mer3 is an evolutionarily conserved DNA helicase that has crucial roles in meiotic recombination and crossover formation. We have identified the MER3 homolog in Coprinus cinereus (Ccmer3) and show that it is expressed in zygotene and pachytene meiocytes. Immunostaining analysis indicated that CcMer3 was localized on chromosomes at zygotene and pachytene and CcMer3 foci were more frequent on paired than unpaired chromosomes. We generated a C. cinereus mer3 mutant (#1) and found that it showed abnormal meiosis progression and underwent apoptosis after prophase I. Basidiospore production in #1 was reduced to 0.8% of the wild-type level; the spores showed slower germination at 25 degrees C but were similar to the wild type at 37 degrees C. Electron microscopic analysis of chromosome spreads revealed that axial elements were formed in the mutant but that synapsis was defective, resulting in a reduction in spore production. Our results demonstrate that CcMer3 is required for synaptonemal complex formation after axial elements align and is thus essential for homologous synapsis.

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HFM1, the human homologue of yeast Mer3, encodes a putative DNA helicase expressed specifically in germ-line cells

Cited by 38 publications

References 15 publications

OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice

OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice

Serial Analysis of Gene Expression Identifies Connective Tissue Growth Factor Expression as a Prognostic Biomarker in Gallbladder Cancer

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

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