<i>HANABA TARANU</i>regulates the shoot apical meristem and leaf development in cucumber (<i>Cucumis sativus</i>L.)

Ding, Lian; Yan, Shuangshuang; Jiang, Li; Liu, Meiling; Zhang, Juan; Zhao, Jianyu; Zhao, Wensheng; Han, Yingyan; Wang, Qian; Zhang, Xiaolan

doi:10.1093/jxb/erv409

Cited by 41 publications

(42 citation statements)

References 71 publications

(95 reference statements)

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“…To further explore the function of CsLFY in cucumber, the 35S promoter followed by double‐stranded RNAi construct containing the 29–393 bp coding sequence of CsLFY ( CsLFY‐ RNAi) was transformed into the cucumber inbred line R1461 as previously described (Ding et al ., ). Six transgenic lines were obtained and three representative lines (#13: weak, #21: medium, #53: strong) were chosen for further analyses (Fig.…”

Section: Resultsmentioning

confidence: 99%

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CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)

et al. 2017

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Cucumber (Cucumis sativus) is an agronomically important vegetable with indeterminant growth habit, in which leaves are produced from the shoot apical meristem (SAM), and unisexual flowers are generated from the leaf axils. LEAFY (LFY) and its homologs have been shown to play important roles in promoting flower development and branching. The LFY homolog gene CsLFY was cloned in cucumber. Molecular biology, developmental biology and biochemical tools were combined to explore the biological function of the LFY homologous gene CsLFY in cucumber. CsLFY was expressed in the SAM, floral meristem and floral organ primordia. Ectopic expression of CsLFY rescued the phenotype of the lfy-5 mutant in Arabidopsis. Knockdown of CsLFY by RNA interference (RNAi) led to defective shoot development and premature discontinuance of leaf initiation in cucumber. Transcription of CsWUS and putative CsLFY target genes including CsAP3 and CUM1 were significantly reduced in the CsLFY-RNAi lines. Further biochemical analyses indicated that CsLFY physically interacts with CsWUS in cucumber. These data suggested that CsLFY has a novel function in regulating shoot meristem maintenance through interaction with CsWUS, and promotes flower development via activation of CsAP3 and CUM1 in cucumber.

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Section: Resultsmentioning

confidence: 99%

“…The empty PFGC‐1008 vector was used as a control. The recombinant CsLFY ‐RNAi construct and the empty PFGC‐1008 vector were transferred into Agrobacterium and then transformed into cucumber inbred line R1461 as previously described (Ding et al ., ). The primers used for vector construction are detailed in Table S1.…”

Section: Methodsmentioning

confidence: 97%

CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)

et al. 2017

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“…At 3, 5, 7, 9, 11 and 13 days after a reciprocal cross between B73 and Mo17, kernels were fixed, embedded, sectioned and dewaxed as described (Ding et al ., ). The 10‐μm thick sections were mounted in neutral resin, de‐waxed and stained with hematoxylin staining solution for 5 min (Muhlhausen et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Sequential gene activation and gene imprinting during early embryo development in maize

Meng

Zhao

et al. 2018

The Plant Journal

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Gene imprinting is a widely observed epigenetic phenomenon in maize endosperm; however, whether it also occurs in the maize embryo remains controversial. Here, we used high-throughput RNA sequencing on laser capture microdissected and manually dissected maize embryos from reciprocal crosses between inbred lines B73 and Mo17 at six time points (3-13 days after pollination, DAP) to analyze allelic gene expression patterns. Co-expression analysis revealed sequential gene activation during maize embryo development. Gene imprinting was observed in maize embryos, and a greater number of imprinted genes were identified at early embryo stages. Sixty-four strongly imprinted genes were identified (at the threshold of 9:1) on manually dissected embryos 5-13 DAP (more imprinted genes at 5 DAP). Forty-one strongly imprinted genes were identified from laser capture microdissected embryos at 3 and 5 DAP (more imprinted genes at 3 DAP). Furthermore, of the 56 genes that were completely imprinted (at the threshold of 99:1), 36 were not previously identified as imprinted genes in endosperm or embryos. In situ hybridization demonstrated that most of the imprinted genes were expressed abundantly in maize embryonic tissue. Our results shed lights on early maize embryo development and provide evidence to support that gene imprinting occurs in maize embryos.

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“…Images of fresh samples and cleared petals were photographed using a S8AP0 light microscope (Leica Camera AG, Germany). Petals sampled at the SEB stage were fixed, embedded, sectioned and de-waxed as described elsewhere (Ding et al, 2015). Sections were imaged using a DM1000 microscope (Leica Camera AG, Germany).…”

Section: Morphological and Histological Analysismentioning

confidence: 99%

Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

Ding

Zhao

Zhang

et al. 2019

Plant Biotechnology Journal

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Summary The diversity of form of the chrysanthemum flower makes this species an ideal model for studying petal morphogenesis, but as yet, the molecular mechanisms underlying petal shape development remain largely unexplored. Here, a floral mutant, which arose as a bud sport in a plant of the variety ‘Anastasia Dark Green’, and formed straight, rather than hooked petals, was subjected to both comparative morphological analysis and transcriptome profiling. The hooked petals only became discernible during a late stage of flower development. At the late stage of ‘Anastasia Dark Green’, genes related to chloroplast, hormone metabolism, cell wall and microtubules were active, as were cell division‐promoting factors. Auxin concentration was significantly reduced, and a positive regulator of cell expansion was down‐regulated. Two types of critical candidates, boundary genes and adaxial–abaxial regulators, were identified from 7937 differentially expressed genes in pairwise comparisons, which were up‐regulated at the late stage in ‘Anastasia Dark Green’ and another two hooked varieties. Ectopic expression of a candidate abaxial gene, CmYAB1, in chrysanthemum led to changes in petal curvature and inflorescence morphology. Our findings provide new insights into the regulatory networks underlying chrysanthemum petal morphogenesis.

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HANABA TARANUregulates the shoot apical meristem and leaf development in cucumber (Cucumis sativusL.)

Abstract: HighlightFunctional analysis of cucumber CsHAN1 showed that it regulates meristem development through WUSCHEL and SHOOT MERISTEMLESS pathways, and mediates leaf development through a complicated gene regulatory network in cucumber.

Cited by 41 publications

References 71 publications

CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)

CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)

Sequential gene activation and gene imprinting during early embryo development in maize

Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

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