gyrA
 Polymorphism in
 Campylobacter jejuni
 : Detection of
 gyrA
 Mutations in 162
 C. jejuni
 Isolates by Single-Strand Conformation Polymorphism and DNA Sequencing

Hakanen, Antti; Jalava, Jari; Kotilainen, Pirkko; Jousimies‐Somer, Hannele; Siitonen, Anja; Huovinen, Pentti

doi:10.1128/aac.46.8.2644-2647.2002

Cited by 53 publications

(56 citation statements)

References 27 publications

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“…BECKMANN et al (2004) reported the same silent mutations detected in this research, His-81-His and Ser-119-Ser, in quinolone sensitive strains. The silent mutation Ala-120-Ala, found in this study, was also previously described by WILSON et al (2000) and HAKANEN et al (2002).…”

Section: Discussionsupporting

confidence: 66%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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Section: Discussionsupporting

confidence: 66%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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“…These data, together with those recently published from Kuwait (Albert et al, 2005), show that, similar to other geographical locations (Zirnstein et al, 1999;Engberg et al, 2001;Hakanen et al, 2002;Dionisi et al, 2004;Beckmann et al, 2004), this mutation is the one most commonly encountered in fluoroquinolone-resistant isolates in the Middle East. Extensive polymorphism of the gyrA gene in C. jejuni within as well as outside the QRDR is well documented (Dionisi et al, 2004;Beckmann et al, 2004;Hakanen et al, 2002;Piddock et al, 2003). Based on partial sequences, the 35 fluoroquinolone-resistant strains encountered in this study carried 10 allelic variants of the gyrA gene (G1-G10), although distributed disproportionately (Fig.…”

mentioning

confidence: 74%

High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates

Sonnevend

Rotimi

Kolodziejek

et al. 2006

Journal of Medical Microbiology

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The antibiotic sensitivity and the serotype and molecular type (MT) distribution of 41 Campylobacter jejuni strains isolated from individual patients in Tawam Hospital, Al Ain, United Arab Emirates, were investigated. While all strains were sensitive to erythromycin (MIC 0?5-4 mg l "1 ), 35 isolates (85?4 %) exhibited resistance to ciprofloxacin (MIC 8-64 mg l "1 ). All resistant strains carried the Thr-86 to Ile mutation in the gyrase A (gyrA) gene, as shown by mismatch amplification mutation assay (MAMA) and confirmed by sequencing. Based on the partial sequences of gyrA, resistant isolates carried 10 distinct alleles, eight of them representing new variants. Strains were assigned to 30 MTs based on the combined results of PFGE and flaA PCR-RFLP typing. Eight of the 35 ciprofloxacin-resistant strains, isolated over a period of more than 1 year, represented the largest MT, also carrying the same allelic variant of the gyrA gene. These results show that the local incidence of fluoroquinolone resistance among C. jejuni is one of the highest reported worldwide. It was also demonstrated that stable MTs could persist for a relatively long time among the clonally unrelated antibiotic-resistant isolates of C. jejuni. The data also emphasize the need to replace fluoroquinolones as empirical therapy for diarrhoea of undiagnosed aetiology. INTRODUCTIONFluoroquinolone resistance in Campylobacter jejuni, a leading cause of gastrointestinal infections worldwide and one of the most important causes of travellers' diarrhoea, is rapidly increasing. Resistance is commonly due to point mutations in the quinolone resistance determinant region (QRDR) of the gyrase A (gyrA) gene involving the Thr-86 for high, and the Asp-90 and Ala-70 amino acid residues for lower-level resistance (Engberg et al., 2001).While C. jejuni infections usually present as acute selflimiting gastrointestinal illnesses, in severe or prolonged cases antibiotic therapy is indicated, with macrolides as the primary drug of choice (Butzler, 2004). However, particularly if the causative agent is not identified, as is often the case in travellers' diarrhoea, fluoroquinolones are frequently used empirically (Guerrant et al., 2001;Yates, 2005). In such cases, resistance to ciprofloxacin can result in therapeutic failure (Guerrant et al., 2001;Sanders et al., 2002). Moreover, by mechanisms yet to be elucidated, fluoroquinolone resistance appears to coincide with increased fitness of the organism. In humans, fluoroquinolone-resistant strains are shed longer than sensitive isolates (Nelson et al., 2004;Engberg et al., 2004), and they have been reported to out-compete their sensitive counterparts, at least in certain genetic backgrounds, when colonizing poultry, i.e. the natural reservoir of the pathogen (Luo et al., 2005). All these findings underscore the necessity for close monitoring of the incidence and spread of fluoroquinolone-resistant clones of C. jejuni. Campylobacter isolates recovered from the 3389 stool samples tested during this period of time. Seven of th...

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“…To investigate the molecular basis of high FQ resistance in the C. jejuni isolates, we analyzed the full gene sequences encoding the DNA gyrase subunits (gyrA and gyrB) (9,10) and the topoisomerase IV subunits (parC and parE) (11,12), which are known targets of FQ, the regulatory protein CmeR, and the sequence of the promoter region of cmeABC, which encodes an efflux pump (13,14), by DNA sequencing as previously described (15,16). The MICs of ciprofloxacin against the 39 isolates which represent different sequence variants were tested according to CLSI guidelines (17,28).…”

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confidence: 99%

Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

Zhāng

Cheng

Luo

et al. 2017

Antimicrob Agents Chemother

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Sequence analysis of 79 ciprofloxacin-resistant Campylobacter jejuni isolates collected in China showed resistance-related sequence variations in gyrA and CmeR-Box. All the isolates contain an identical Thr-86-Ile substitution in GyrA. Several novel CmeR-Box variations, including point substitutions, deletion, and insertion, were identified. The point insertion or deletion led to dramatically reduced binding of CmeR to the cmeABC promoter, which significantly increases the expression of cmeABC and contributes to the high fluoroquinolone resistance.

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gyrA Polymorphism in Campylobacter jejuni : Detection of gyrA Mutations in 162 C. jejuni Isolates by Single-Strand Conformation Polymorphism and DNA Sequencing

Cited by 53 publications

References 27 publications

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates

Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

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