<i>Growing Slowly 1</i>locus encodes a PLS-type PPR protein required for RNA editing and plant development in Arabidopsis

Xie, Tingting; Chen, Dan; Wu, Jian; Huang, Xiaorong; Wang, Yifan; Tang, Kun; Li, Jiayang; Sun, Mingwei; Peng, Xiongbo

doi:10.1093/jxb/erw331

Cited by 37 publications

(24 citation statements)

References 66 publications

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“…So far, the only PPR mutant, which showed lower sensitivity to ABA is ppr96 (Liu et al ., ), in which the ABI5 expression level is downregulated. In contrast, other PPR protein mutants for example abo5 (Liu et al ., ), pgn (Laluk et al ., ), soar1‐2 (Mei et al ., ), abo8 (Yang et al ., ) and grs1 (Xie et al ., ) showed a higher ABA sensitivity compared with the corresponding wild type plants.…”

Section: Discussionmentioning

confidence: 84%

“…Plant PPR proteins are split into two main classes based on their characteristic domain architecture: P‐class and PLS‐class (Schmitz‐Linneweber and Small, ) and are mostly targeted to the mitochondria and chloroplasts (Barkan and Small, ). To date, few PPR proteins are known to be implicated in different stress responses and plant development for example PPR40, SLO1, GUN1, ABO5, PGN, SLG1, AtGRS1 and PPR96 (Zsigmond et al ., ; Cottage et al ., ; Liu et al ., , ; Sung et al ., ; Laluk et al ., ; Yuan and Liu, ; Xie et al ., ). Furthermore, several PPR mutants that have altered sensitivity to ABA show defects in RNA processing, for example in RNA editing (Liu et al ., ; He et al ., ; Zhu et al ., ; Sechet et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

Emami

Kempken

2019

The Plant Journal

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Flowering is a vital developmental shift in plants from vegetative to reproductive phase. The timing of this shift is regulated by various linked genetic pathways including environmental cues and internal regulation. Here we report a role for an Arabidopsis gene, AT1G15480, which encodes a P-class pentatricopeptide repeat (PPR) protein, affecting flowering time. We show that AT1G15480 is localized to mitochondria. An AT1G15480 T-DNA insertion line exhibits an early-flowering phenotype, which is quite a rare phenotype among PPR mutants. The early-flowering phenotype was observed under both long and short days compared with wild type plants. Genetic complementation confirmed the observed phenotype. We therefore named the PPR protein PRECOCIOUS1 (POCO1). poco1 plants showed lower respiration, ATP content and higher accumulation of superoxide. Importantly, the quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that the expression of FLOWERING LOCUS C (FLC), which is a key floral repressor, was strongly downregulated in the poco1. Likewise, the expression level of the FLC positive regulator ABSCISIC ACID-INSENSITIVE 5 (ABI5) was reduced in the poco1. Consistent with the qRT-PCR results, poco1 plants showed reduced sensitivity to abscisic acid compared with wild type with respect to primary root growth and days to flowering. Furthermore, the poco1 mutation enhances the sensitivity to drought stress. Further analysis showed that POCO1 affects mitochondrial RNA editing. Taken together, our data demonstrate a remarkable function of POCO1 in flowering time and the abscisic acid signalling pathway.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 97%

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

Emami

Kempken

2019

The Plant Journal

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“…Essentially, the role of delineated most-promising SYP trait-associated PPR genes with synonymous SNPs in regulating fertility restoration, heterosis breeding and hybrid seed production is well-documented in crop plants including legumes 52,54,56,57 . Modulation of their gene expression can thereby enhance the overall seed yield.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide generation and genotyping of informative SNPs to scan molecular signatures for seed yield in chickpea

Basu

Srivastava

Bajaj

et al. 2018

Sci Rep

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We discovered 2150 desi and 2199 kabuli accessions-derived SNPs by cultivar-wise individual assembling of sequence-reads generated through genotyping-by-sequencing of 92 chickpea accessions. Subsequent large-scale validation and genotyping of these SNPs discovered 619 desi accessions-derived (DAD) SNPs, 531 kabuli accessions-derived (KAD) SNPs, 884 multiple accessions-derived (MAD) SNPs and 1083 two accessions (desi ICC 4958 and kabuli CDC Frontier)-derived (TAD) SNPs that were mapped on eight chromosomes. These informative SNPs were annotated in coding/non-coding regulatory sequence components of genes. The MAD-SNPs were efficient to detect high intra-specific polymorphic potential and wide natural allelic diversity level including high-resolution admixed-population genetic structure and precise phylogenetic relationship among 291 desi and kabuli accessions. This signifies their effectiveness in introgression breeding and varietal improvement studies targeting useful agronomic traits of chickpea. Six trait-associated genes with SNPs including quantitative trait nucleotides (QTNs) in combination explained 27.5% phenotypic variation for seed yield per plant (SYP). A pentatricopeptide repeat (PPR) gene with a synonymous-coding SNP/QTN significantly associated with SYP trait was found most-promising in chickpea. The essential information delineated can be of immense utility in genomics-assisted breeding applications to develop high-yielding chickpea cultivars.

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“…EMP5 participates in C-to-U editing of mitochondrial multiple sites, especially at rpl16-458 (Liu et al, 2013). Recently, GRS1 was found to edit Cs at nad1-265, nad4L-55, nad6-103 and rps4-377 in Arabidopsis mitochondria (Xie et al, 2016). Apart from DYW-PPR proteins, several E-PPR proteins have also been considered to be related to RNA editing at one or several sites in plant mitochondrial transcripts (Takenaka et al, 2010;Hammani et al, 2011;Murayama et al, 2012;Yuan & Liu, 2012;Zhu et al, 2012;Li et al, 2014;Sun F. et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

EMP18 functions in mitochondrial atp6 and cox2 transcript editing and is essential to seed development in maize

Huang

Yang

et al. 2018

New Phytologist

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RNA editing plays an important role in organellar gene expression in plants, and pentatricopeptide repeat (PPR) proteins are involved in this function. Because of its large family size, many PPR proteins are not known for their function and roles in plant growth and development. Through genetic and molecular analyses of the empty pericarp18 (emp18) mutant in maize (Zea mays), we cloned the Emp18 gene, revealed its molecular function, and defined its role in the mitochondrial complex assembly and seed development. Emp18 encodes a mitochondrial-localized DYW-PPR protein. Null mutation of Emp18 arrests embryo and endosperm development at an early stage in maize, resulting in embryo lethality. Mutants are deficient in the cytidine (C)-to-uridine (U) editing at atp6-635 and cox2-449, which converts a Leu to Pro in ATP6 and a Met to Thr in Cox2. The atp6 gene encodes the subunit a of F F -ATPase. The Leu to Pro alteration disrupts an α-helix of subunit a, resulting in a dramatic reduction in assembly and activity of F F -ATPase holoenzyme and an accumulation of free F -subcomplex. These results demonstrate that EMP18 functions in the C-to-U editing of atp6 and cox2, and is essential to mitochondrial biogenesis and seed development in maize.

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Growing Slowly 1locus encodes a PLS-type PPR protein required for RNA editing and plant development in Arabidopsis

Abstract: HighlightThe mitochondrial pentatricopeptide repeat protein AtGRS1 edits RNA at four sites and is critical for development. The abscisic acid response gene ABI5 participates in the short root phenotype of grs1.

Cited by 37 publications

References 66 publications

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana

Genome-wide generation and genotyping of informative SNPs to scan molecular signatures for seed yield in chickpea

EMP18 functions in mitochondrial atp6 and cox2 transcript editing and is essential to seed development in maize

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