<i>Gordona</i>: A Rare Cause of Peritoneal Dialysis Peritonitis

Imran, Muhammad; Livesley, P. J.; Bell, G. H.; Pai, Pearl; Neal, Timothy; Anijeet, Hameed

doi:10.3747/pdi.2011.00150

Cited by 7 publications

(12 citation statements)

References 9 publications

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“…Although Gordonia species have been implicated in a variety of infections, only seven cases of continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis caused by Gordonia species have been described (1)(2)(3)(4). Furthermore, the methods used for identifying these seven Gordonia isolates were not mentioned in five cases (2,4), and the accuracy of the identifications could not be ascertained.…”

mentioning

confidence: 95%

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Lam

Leung³

et al. 2015

J Clin Microbiol

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We report here four cases of continuous ambulatory peritoneal dialysis-related peritonitis caused by three different species of Gordonia. The portal of entry was likely through Tenckhoff catheters. 16S rRNA and secA1 gene sequencing are so far the most reliable methods for the accurate identification of Gordonia species. Gordonia species are Gram-positive weakly acid-fast coryneform bacteria. Although Gordonia species have been implicated in a variety of infections, only seven cases of continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis caused by Gordonia species have been described (1-4). Furthermore, the methods used for identifying these seven Gordonia isolates were not mentioned in five cases (2, 4), and the accuracy of the identifications could not be ascertained. In this article, we described four cases of CAPD-related peritonitis caused by three different species of Gordonia confirmed by 16S rRNA and secA1 gene sequencing.Clinical specimens were collected and handled according to standard protocols, and the clinical data were collected by analyzing the patients' hospital records. Phenotypic identification was performed using standard conventional biochemical methods and the API Coryne system (bioMérieux, France). All tests were performed in triplicate. The MICs were determined using Etests (bioMérieux), according to the standards of the Clinical and Laboratory Standards Institute (CLSI) (5), with Escherichia coli strain ATCC 25922 and Pseudomonas aeruginosa strain ATCC 27853 as controls; the results were compared with the CLSI MIC interpretive standards for Staphylococcus spp. and Streptococcus pneumoniae (6) for evaluation. Bacterial DNA extraction, PCR amplification, and DNA sequencing of the 16S rRNA and secA1 genes were performed according to our previous publication (7), except for the primer pairs used (G268F/G1096R [8] for the 16S rRNA gene and SecA1-f/SecA1-r [9] for the secA1 gene). Comparative sequence identity analysis and phylogenetic analysis using the maximum likelihood method were performed according to our previous publication (10), except that MEGA 6.06 (11) was used instead. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was performed according to our previous publication (12), and the protein profiles obtained were processed and analyzed using MALDI Biotyper 3.1 (Bruker Daltonics, Germany) for the generation of the hierarchical cluster analysis (HCA) dendrogram and for identification. Case 1. A 65-year-old Chinese man was admitted for cloudy dialysis effluent for 1 day. He had end-stage renal failure (ESRF) of unknown etiology and had been undergoing CAPD for 7 years. He was afebrile but had turbid dialysis effluent. The total leukocyte count of the dialysis fluid was 930/mm 3 , with neutrophil predominance. A Gram smear of the dialysis effluent after centrifugation revealed numerous leukocytes without any organisms. Empirical intraperitoneal (i.p.) cefazolin and gentamicin therapies were started. As the clinical condition of...

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confidence: 95%

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Lam

Leung³

et al. 2015

J Clin Microbiol

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“…In patients undergoing peritoneal dialysis (PD), Gordonia species can rarely cause peritonitis. [1][2][3] Nonetheless, the natural history, optimal treatment, and outcome of Gordonia peritonitis are not well described in the literature. In the past 10 years, we encountered four cases of Gordonia peritonitis in our centre.…”

Section: Tions In Patients Undergoing Peritoneal Dialysis Gordonia mentioning

confidence: 99%

“…In the past 10 years, we encountered four cases of Gordonia peritonitis in our centre. In addition, we identified only three case reports of Gordonia peritonitis in literature search . Here, we report the four Chinese PD patients with peritonitis caused by Gordonia species in our centre.…”

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Peritoneal‐dialysis related peritonitis caused by Gordonia species: Report of four cases and literature review

et al. 2014

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“…They are followed by Gram-negative organisms and fungi. However, recently there have been increasing reports of rare and unusual organisms causing peritonitis in patients maintained on PD (Imran et al ., 2012; Kahveci et al ., 2011). Strains of Rhizobium spp.…”

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confidence: 99%

Rhizobium radiobacter peritonitis: the first case report from India and review

et al. 2014

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Background:Rhizobium radiobacter is an opportunistic human pathogen in debilitated patients with foreign plastic intravascular devices and peritoneal dialysis (PD) catheters. We performed a Medline search of the English-language literature on R. radiobacter continuous ambulatory peritoneal dialysis (CAPD) peritonitis in end-stage renal disease (ESRD) and reviewed 13 cases.Case presentation:A 54-year-old male with ESRD secondary to chronic long-standing type II diabetes mellitus presented to the nephrology outpatient department with fever, abdominal pain and cloudy PD effluent. The patient was admitted to the hospital. PD fluid was sent for cell count, Gram stain and culture. The white blood cell (WBC) count in the PD fluid was 6400 mm−3 with 82 % neutrophils. Gram staining of the fluid showed plenty of Gram-negative bacilli. A presumptive diagnosis of CAPD peritonitis was made and empiric intraperitoneal cefazolin and tobramycin were started. The PD fluid culture grew non-fermenting, Gram-negative bacilli identified as R. radiobacter. Empiric antibiotic therapy was continued and the patient’s abdominal pain subsided. The peritoneal fluid counts decreased to 50 WBCs mm−3 on day 4. However, his abdominal pain recurred on day 8 and his PD fluid count increased to 300 cells mm−3. The catheter was therefore surgically removed.Conclusion:Although R. radiobacter is considered a contaminant, it can cause relapsing symptomatic peritonitis peritoneal catheter removal.

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Gordona: A Rare Cause of Peritoneal Dialysis Peritonitis

Cited by 7 publications

References 9 publications

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Peritoneal‐dialysis related peritonitis caused by Gordonia species: Report of four cases and literature review

Rhizobium radiobacter peritonitis: the first case report from India and review

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