GAS1Deficient Enhances UPR Activity inSaccharomyces cerevisiae

Abstract

Beta-1,3-glucanosyltransferase (Gas1p) plays important roles in cell wall biosynthesis and morphogenesis and has been implicated in DNA damage responses and cell cycle regulation in fungi. Yeast Gas1p has also been reported to participate in endoplasmic reticulum (ER) stress responses. However, the precise roles and molecular mechanisms through which Gas1p affects these responses have yet to be elucidated. In this study, we constructed GAS1-deficient (gas1Δ) and GAS1-overexpressing (GAS1 OE) yeast strains and observed that the gas1Δ strain exhibited a decreased proliferation ability and a shorter replicative lifespan (RLS), as well as enhanced activity of the unfolded protein response (UPR) in the absence of stress. However, under the high-tunicamycin-concentration (an ER stress-inducing agent; 1.0 μg/mL) stress, the gas1Δ yeast cells exhibited an increased proliferation ability compared with the wild-type yeast strain. In addition, our findings demonstrated that IRE1 and HAC1 (two upstream modulators of the UPR) are required for the survival of gas1Δ yeast cells under the tunicamycin stress. On the other hand, we provided evidence that the GAS1 overexpression caused an obvious sensitivity to the low-tunicamycin-concentration (0.25 μg/mL). Collectively, our results indicate that Gas1p plays an important role in the ageing and ER stress responses in yeast.

Single-cell and spatial mapping Identify cell types and signaling Networks in the human ureter

“…In fact, the top marker gene for this cluster, growth arrest specific-1 (GAS1), is an anti-mitogenic factor that mediates the growth arrest of fibroblasts. The Saccharomyces cerevisiae homolog of GAS1 plays an important role in the endoplasmic reticulum stress response (Cui et al, 2019). Thus, the minor interstitial fibroblasts might play a role in upregulating pathways involved in protein homeostasis.…”

Ureter single-cell and spatial mapping reveal cell types, architecture, and signaling networks

“…In fact, growth arrest specific-1 ( GAS1 ) is an anti-mitogenic factor that mediates growth arrest of fibroblasts. The Saccharomyces cerevisiae homologue of GAS1 plays an important role in the endoplasmic reticulum stress response (Cui et al, 2019). Thus, this population of fibroblasts might play a role in upregulating pathways involved in protein homeostasis.…”

GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast

“…2000 ; Levin 2005 ). In addition to the established function in the cell wall, it was reported that GAS1 may play roles in several intracellular processes such as DNA damage response ( Eustice and Pillus 2014 ), locus-specific transcriptional silencing ( Koch and Pillus 2009 ), and ER stress response ( Cui et al . 2019 ).…”

“…2003 ; Koch and Pillus 2009 ), it has been proposed that Gas1 is involved in post-translational glycosylation of putative chromatin components ( Koch and Pillus 2009 ; Eustice and Pillus 2014 ). Loss of GAS1 also elevates the unfolded protein response (UPR) and renders the mutant cells resistant to tunicamycin-induced ER proteotoxic stress ( Cui et al . 2019 ).…”