1990
DOI: 10.1111/j.1432-1033.1990.tb15542.x
|View full text |Cite
|
Sign up to set email alerts
|

GAP1, the general amino acid permease gene of Saccharomyces cerevisiae

Abstract: In Saccharomyces cerevisiae, mutations at the GAP1 locus selectively abolish the activity of the general amino acid transport system. This permease catalyses active transport of apparently all biological amino acids across the plasma membrane. We have determined the nucleotide sequence of the GAP1 gene. The sequence contains an open reading frame of 601 codons corresponding to a polypeptide of Mr 65578. This polypeptide is strongly hydrophobic; it exhibits three potential glycosylation sites. Hydropathy analys… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

10
170
1

Year Published

1996
1996
2007
2007

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 271 publications
(182 citation statements)
references
References 48 publications
10
170
1
Order By: Relevance
“…3A) and for which NCR is the main (if not the sole) known transcriptional regulation. This category includes GAP1, which codes for the general amino acid permease (67) and has been the focus of many studies aimed at deciphering the molecular mechanisms of NCR (26,85). It also includes three of the four genes (GAT1/NIL1, DAL80/UGA43, GZF3/DEH1/NIL2) coding for the GATA transcription factors involved in the transcriptional control of NCR target genes (26,85), shown in previous studies to be under NCR control and interlinked in a network of trans-and auto-regulation systems (12,23,109,119).…”
Section: General Approachmentioning
confidence: 99%
“…3A) and for which NCR is the main (if not the sole) known transcriptional regulation. This category includes GAP1, which codes for the general amino acid permease (67) and has been the focus of many studies aimed at deciphering the molecular mechanisms of NCR (26,85). It also includes three of the four genes (GAT1/NIL1, DAL80/UGA43, GZF3/DEH1/NIL2) coding for the GATA transcription factors involved in the transcriptional control of NCR target genes (26,85), shown in previous studies to be under NCR control and interlinked in a network of trans-and auto-regulation systems (12,23,109,119).…”
Section: General Approachmentioning
confidence: 99%
“…Upon addition of a preferential nitrogen source like ammonium (NH 4 ϩ ), the GAP1 gene is repressed, and the permease present at the cell surface is rapidly poly-ubiquitinated on two lysine residues in the N-terminal tail (Lys-9 and Lys-16). This ubiquitination triggers Gap1 endocytosis followed by sorting to the vacuole/lysosome lumen for degradation (17)(18)(19)(20)(21)(22). Ubiquitination of Gap1 at the cell surface is dependent on the ubiquitin ligase Rsp5/Npi1, the Bul proteins, and the Doa4/Npi2 ubiquitin hydrolase, the latter being required to maintain a normal level of free ubiquitin (19,20,22,23).…”
mentioning
confidence: 99%
“…Gap1p is a high-capacity permease that can transport all naturally occurring amino acids (5,6). GAP1 transcription is positively regulated by the GATA-type transcription factors Gln3p and Gat1p͞Nil1p and negatively regulated by the cytoplasmic factor Ure2p, so that GAP1 is expressed on nonpreferred nitrogen sources but repressed on preferred nitrogen sources (1).…”
mentioning
confidence: 99%