<i>Ganoderma lucidum</i>(Reishi mushroom) for cancer treatment

Jin, Xingzhong; Beguerie, Julieta Ruíz; Sze, Daniel Man-yeun; Chan, Godfrey C. F.

doi:10.1002/14651858.cd007731.pub2

Cited by 79 publications

(56 citation statements)

References 28 publications

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“…The Cochrane collaboration presented by Jin et al (2012) also reviewed the benefit of lingzhi in terms of cancer treatment. They summarized that the evidence of using lingzhi as a first line treatment for cancer was insufficient to prolong long term survival.…”

Section: Discussionmentioning

confidence: 99%

“…They summarized that the evidence of using lingzhi as a first line treatment for cancer was insufficient to prolong long term survival. However, it could be administered as an alternative adjunct to conventional treatment for enhancing tumor response and stimulating host immunity (Jin et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Ganoderma lucidum which is commonly known as "Lingzhi" is a woody Basidiomycetes mushroom in the Gonodermaceae or Aphyllophorals family (Jin et al, 2012). It has been widely used in China and other Asian countries in health promotion for thousands of years (Martínez-Montemayor et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Clinical Characteristics of Gynecologic Cancer Patients who Respond to Salvage Treatment with Lingzhi

Suprasert

Apichartpiyakul²,

Sakonwasun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Lingzhi or Ganoderma lucidum is a popular medicinal mushroom used as a health promotion herb in China and other Asian countries for thousands of years. There have many previous studies about the anti-cancer effects of lingzhi especially in vitro. The present study reports the clinical data of 5 gynecologic cancer patients who achieved stability in the disease after ingestion of lingzhi in the form of fruit body water extract and spores in a salvage setting. This report has been written to enhance the data describing the effect of lingzhi in cancer patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Clinical Characteristics of Gynecologic Cancer Patients who Respond to Salvage Treatment with Lingzhi

Suprasert

Apichartpiyakul²,

Sakonwasun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…However, this treatment remains ineffective due to its toxicity to normal cells (2). Natural medicine has been widely used for treating malignant tumors for thousands of years (3,4). There is great potential in identifying drugs from natural medicine for the treatment of metastatic breast cancer due to their wide range of biological activities and low toxicity.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of emodin on migration, invasion and metastasis of human breast cancer MDA-MB-231 cells in vitro and in vivo

et al. 2014

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Abstract. In breast cancer, metastasis is the main reason for patient mortality. In the present study, we used breast cancer MDA-MB-231 cells and a mouse xenograft model to demonstrate the effect of emodin on the migration, invasion and metastasis of human breast cancer MDA-MB-231 cells and the related mechanisms. In vitro, wound healing and Transwell assays showed that emodin dose-dependently inhibited the migration and invasion of MDA-MB-231 cells. Enzyme-linked immunosorbent assay (ELISA) showed that emodin decreased the secretion of MMP-2 and MMP-9. Western blot analysis showed that emodin downregulated the expression levels of MMP-2, MMP-9, uPA and uPAR as well as p38 inhibitor SB203580 and ERK inhibitor PD980559, even though TIMP-1 and TIMP-2 were not obviously changed in the MDA-MB-231 cells. Furthermore, emodin inhibited the activity of p38 and ERK1/2 in the MDA-MB-231 cells. In vivo, emodin inhibited lung metastasis in mice bearing the breast cancer MDA-MB-231 xenografts with no obvious changes in body weight, liver and kidney functions. These results indicated that emodin inhibited the lung metastasis of human breast cancer in a mouse xenograft model, and inhibited the invasion of MDA-MB-231 cells associated with the downregulation of MMP-2, MMP-9, uPA and uPAR expression as well as decreased activity of p38 and ERK.

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“…A scientific review has indicated that the Ganoderma mushroom produces beneficial effects as an adju-vant agent for cancer treatment. 13) In addition, G. lucidum also improves the quality of life [the International Prostate Symptom Score (IPSS)] of men who have lower urinary tract symptoms. 14) To date, pharmacologically active compounds from the Ganoderma species have been found to be polysaccharides and triterpenes.…”

mentioning

confidence: 99%

Bacterial Expression of a Single-Chain Variable Fragment (scFv) Antibody against Ganoderic Acid A: A Cost-Effective Approach for Quantitative Analysis Using the scFv-Based Enzyme-Linked Immunosorbent Assay

Yusakul

Nuntawong

Sakamoto

et al. 2017

Biological & Pharmaceutical Bulletin

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Due to the highly specific binding between an antibody and its target, superior analytical performances was obtained by immunoassays for phytochemical analysis over conventional chromatographic techniques. Here, we describe a simple method for producing a functional single-chain variable fragment (scFv) antibody against ganoderic acid A (GAA), a pharmacologically active metabolite from Ganoderma lingzhi. The Escherichia coli BL21(DE3) strain produced a large amount of anti-GAA scFv. However, in vitro refolding steps, which partially recovered the reactivity of the scFv, were required. Interestingly, the functional scFv was expressed as a soluble and active form in the cytoplasm of an engineered E. coli SHuffle ® strain. Purified anti-GAA scFv, which yielded 2.56 mg from 1 L of culture medium, was obtained from simple and inexpensive procedures for expression and purification. The anti-GAA scFv-based indirect competitive enzymelinked immunosorbent assay (icELISA) exhibited high sensitivity (linearity: 0.078-1.25 µg/mL) with precision (CV: ≤6.20%) and reliability (recovery: 100.1-101.8%) for GAA determination. In summary, the approach described here is an inexpensive, simple, and efficient expression system that extends the application of anti-GAA scFv-based immunoassays. In addition, when in vitro refolding steps can be skipped, the cost and complexity of scFv antibody production can be minimized.Key words Ganoderma lingzhi; ganoderic acid A; single-chain variable fragment antibody; Escherichia coli; enzyme-linked immunosorbent assay Antibodies are the most frequently used biological agents for routine diagnostics, therapeutics, and studies in various fields. Their applications to chemical analysis provide various advantages such as binding specificity and methodological simplicity that extend beyond conventional chromatographic techniques. The subjects of target molecules for immunoassays have been expanded from large molecules (i.e., protein, peptide, and DNA) to small molecules (herbicides, natural products, and phytohormones). As such, many monoclonal antibodies (mAbs) against small molecules (haptens), such as amikacin, 1) carbamazepine, 2) aflatoxins, 3) and daidzin, 4,5) have been produced to develop mAb-based immunoassays for qualitative and quantitative analyses, which were shown to be simple and convenient analytical methods. Because the cost of recombinant antibodies (rAbs) produced using Escherichia coli is much lower than that of antibodies produced using hybridoma cells or other animal cell lines, 6) immunoassays using E. coli-derived antibodies represent an economical approach for analytical applications. However, the E. coli-based production of rAbs against haptens is limited by an inability to retain rAb reactivity. Normally, refolding steps are required to recover the binding reactivity of the rAb, and the steps are determined by time-consuming trial-and-error procedures. The single-chain variable fragment (scFv) antibody is a simple and small arrangement of the functional rAb, in which the vari...

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Ganoderma lucidum(Reishi mushroom) for cancer treatment

Abstract: placebocontrolled, multicenter study of Ganoderma lucidum (w.curt.:fr.) Lloyd (aphyllophoromycetideae) polysaccharides (ganopoly) in patients with advanced lung cancer.

Cited by 79 publications

References 28 publications

Clinical Characteristics of Gynecologic Cancer Patients who Respond to Salvage Treatment with Lingzhi

Clinical Characteristics of Gynecologic Cancer Patients who Respond to Salvage Treatment with Lingzhi

Inhibitory effect of emodin on migration, invasion and metastasis of human breast cancer MDA-MB-231 cells in vitro and in vivo

Bacterial Expression of a Single-Chain Variable Fragment (scFv) Antibody against Ganoderic Acid A: A Cost-Effective Approach for Quantitative Analysis Using the scFv-Based Enzyme-Linked Immunosorbent Assay

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