Fusobacterium nucleatumenhances invasion of human gingival epithelial and aortic endothelial cells byPorphyromonas gingivalis

Abstract

Invasion by Porphyromonas gingivalis has been proposed as a possible mechanism of pathogenesis in periodontal and cardiovascular diseases. Porphyromonas gingivalis have direct access to the systemic circulation and endothelium in periodontitis patients by transient bacteremia. Periodontitis can be described as one of the predominant polymicrobial infections of humans. In the present study, P. gingivalis strains were tested for their ability to invade a human gingival epithelial cell line (Ca9-22) and human aortic endothelial cells in coinfection with Fusobacterium nucleatum using antibiotic protection assays. Coinfection with F. nucleatum resulted in 2-20-fold increase in the invasion of host cells by P. gingivalis strains. The invasive abilities of P. gingivalis strains were significantly greater when incubated with a F. nucleatum clinical isolate (which possesses strong biofilm-forming ability), than when incubated with a F. nucleatum-type strain. In inhibition assays with metabolic inhibitors, a difference in inhibition profiles was observed between mono- and polymicrobial infections. Collectively, our results suggest that F. nucleatum facilitates invasion of host cells by P. gingivalis. Investigations of polymicrobial infection of host cells should improve our understanding of the role of P. gingivalis in periodontal infection and proatherogenic mechanisms.

Differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by Porphyromonas gingivalis

“…Galactose, however, only partially inhibited F. nucleatum enhanced P. gingivalis invasion [10]. Furthermore, the enhancement of P. gingivalis invasion was not observed for co-infection with A. actinomycetemcomitans, that can also coaggregate with F. nucleatum [22].…”

“…In our previous experiments, we found that there was a modest increase in the invasion efficiencies of P. gingivalis with increasing MOI. Invasion reached a plateau at about 100 MOI [10] . Thus, we used MOI of 100 in all subsequent experiments.…”

“…gingivalis, and reported that Fusobacterium nucleatum, a periodontopathogen strongly implicated in plaque development [9], enhances invasion of human gingival epithelial and aortic endothelial cells by P. gingivalis [10]. In the present study, we used this polymicrobial infection model to evaluate the influence of several major periodontopathogens on P. gingivalis invasion of human gingival epithelial cells.…”

Investigation of Subgingival Profile of Periodontopathic Bacteria Using Polymerase Chain Reaction

“…Periodontitis has been considered to be a biofilm infection 3,17,19) , and the polymicrobial nature of the periodontopathic bacteria in periodontal lesions has received particular attention 7,19,20) . The results of this study indicate that the relationships between coexistence of P. gingivalis, T. forsythia and T. denticola, designated the red complex, and PD or BOP were significant in periodontitis.…”

Inflammation buccale et accidents vasculaires cérébraux ischémiques non fatals

“…Le parodonte représente une « porte d'entrée » pour les agents infectieux parodontaux tels que Actibacillus actinomycetemcomitans et Porphyromonas gingivalis qui ont la capacité d'envahir les cellules épithéliales. Après leur internalisation, ces bactéries à majorité Gram né-gatif sont capables de franchir la membrane cytoplasmiques des cellules adjacentes entrainant une bactériémie [24] et une élévation des paramètres inflammatoires. On observe ainsi une augmentation de la CRP induite par la stimulation de l'interleukine 1-beta (IL1-beta) ou du TNF alpha [25,26] par le LPS.…”