Fusarium tupiensesp. nov., a member of theGibberella fujikuroicomplex that causes mango malformation in Brazil

Abstract

Fusarium tupiense, the main causal agent of mango malformation in Brazil, is described through a combination of morphological, biological and molecular markers. This new species belongs to the Gibberella fujikuroi species complex (GFSC) and has an anamorph morphologically similar to Fusarium mangiferae and F. sterilihyphosum. F. tupiense can be differentiated from other species in the G. fujikuroi species complex on the basis of sexual crosses, amplified fragment length polymorphism (AFLP) markers and partial sequences of the tef1 and tub2 genes. Female fertility for field isolates of F. tupiense appears to be low. PCR with primers specific for the mating type (MAT) alleles and sexual crosses identified this species as heterothallic with two idiomorphs. Female-fertile tester strains were developed for the identification of field strains of this species through sexual crosses.

First report and characterization of Fusarium circinatum, the causal agent of pitch canker in Brazil

“…The species was first described as a special form of F. subglutinans, as it shares the main morphological features with this species, like the presence of polyphialides, production of conidia in the aerial mycelium only in false heads and the absence of chlamydospores (Correll et al, 1991;Nirenberg & O'Donnell et al, 1998). Morphological characteristics typical of F. circinatum like conidiophores on the erect aerial mycelium, polyphialides with more than two conidiogenous openings and sterile coils could be observed, but these characters are shared with species like F. mexicanum, F. pseudocircinatum, F. sterilihyphosum and F. tupiense (Nirenberg & O'Donnell, 1998;Britz et al, 2002;Lima et al, 2012).…”

“…The application of different species concepts allowed for a reliable and consistent identification of this species, which is a member of the GFC, where morphological markers are scarce or sometimes even absent (Kvas et al, 2009;Lima et al, 2012). The species was first described as a special form of F. subglutinans, as it shares the main morphological features with this species, like the presence of polyphialides, production of conidia in the aerial mycelium only in false heads and the absence of chlamydospores (Correll et al, 1991;Nirenberg & O'Donnell et al, 1998).…”

“…A cirrus of ascospores was collected from three perithecia of each fertile cross, suspended in sterile water, and spread on the surface of 2% water agar in a Petri dish. Plates were incubated overnight at 25ºC in the dark and checked for ascospore germination (Lima et al, 2012). Special care was taken to avoid transfer of conidia with ascospores.…”

New Insights into Mango Malformation Disease Epidemiology Lead to a New Integrated Management Strategy for Subtropical Environments

“…nov., resembles F sterilihyphosum morphologically, is phylogenetically distinct from both F. mangiferae and F. sterilihyphosum, and produces a unique teleomorph in the G. fujikuroi complex. F. tupiense causes malfor mation in Brazil (36) and Senegal (67) (Fig. 4).…”

Fusarium species associated with grain sorghum and mungbean in Queensland

“…(Lima et al, 2012) A number of Fusarium species, formerly recognised as F. moniliforme sensu lato have been associated with sorghum stalk rot (Klittich & Leslie, 1992, Leslie, 1991, Leslie, 1995, Tesso et al, 2010. According to Leslie and Marasas (2002) verticillioides (Leslie & Marasas, 2002).…”