FoxN3 is required for craniofacial and eye development of Xenopus laevis

Schuff, Maximilian; Rossner, Antje; Wacker, Stefan; Donow, Cornelia; Gessert, Susanne; Knöchel, Walter

doi:10.1002/dvdy.21007

Cited by 52 publications

(81 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The homologs of RNF2 and HDAC1 in Xenopus have not been cloned. However, two other members of the HDAC complex, xSin3 and xRBD3, have been reported to coimmunoprecipitate with FoxN3, a protein required in craniofacial and eye development (Schuff et al, 2007).…”

Section: Research Articlementioning

confidence: 99%

Prohibitin1 acts as a neural crest specifier in Xenopus development by repressing the transcription factor E2F1

2010

View full text Add to dashboard Cite

SUMMARYProhibitin 1 (phb1), which was initially described as an inhibitor of cell proliferation, is a highly conserved protein found in multiple cellular compartments. In the nucleus it interacts with the transcriptional regulators Rb and E2F1 and controls cell proliferation and apoptosis. Here we unravel an unexpected novel function for phb1 in Xenopus cranial neural crest (CNC) development. Xphb1 is maternally expressed; zygotically expressed neurula stage transcripts accumulate in the CNC and the neural tube. Knockdown of Xphb1 by antisense morpholino injection results in the loss of foxD3, snail2 and twist expression, whereas expression of c-myc, AP-2 and snail1 remains unaffected. Xphb2, its closest relative, cannot substitute for Xphb1, underlining the specificity of Xphb1 function. Epistatic analyses place Xphb1 downstream of c-myc and upstream of foxD3, snail2 and twist. To elucidate which subdomain in Xphb1 is required for neural crest gene regulation we generated deletion mutants and tested their rescue ability in Xphb1 morphants. The E2F1-binding domain was found to be necessary for Xphb1 function in neural crest development. Gain-and loss-of-function experiments reveal that Xphb1 represses E2F1 activity; suppression of E2F1 through Xphb1 is required for twist, snail2 and foxD3 expression in the CNC. With the Xphb1 dependency of a subset of CNC specifiers downstream of c-myc, we have identified a new branching point in the neural crest gene regulatory network.

show abstract

Section: Research Articlementioning

confidence: 99%

Prohibitin1 acts as a neural crest specifier in Xenopus development by repressing the transcription factor E2F1

2010

View full text Add to dashboard Cite

show abstract

“…It has been reported that FOXN3 is required for craniofacial and eye development in Xenopus laevis (3), and that gene inactivation of Foxn3 in mice leads to partial embryonic and postnatal lethality, growth retardation, eye formation defects, dental anomalies, and craniofacial defects (4). At the cellular level, FOXN3 was described as a checkpoint suppressor (CHES1) in yeast (5) and was shown to inhibit protein biosynthesis (6) or to downregulate E2F5 in human cells to control cell cycle (7).…”

Section: Introductionmentioning

confidence: 99%

“…At the cellular level, FOXN3 was described as a checkpoint suppressor (CHES1) in yeast (5) and was shown to inhibit protein biosynthesis (6) or to downregulate E2F5 in human cells to control cell cycle (7). At the molecular level, although it has been reported that FOXN3 interacts with xSin3/xRPD3 in X. laevis (3) and Sin3 in Saccharomyces cerevisiae (8) and with MEN1 (9) or SKIP (10) in human cells to exert transcriptional repressive function, in Drosophila testes it was demonstrated that FOXN3 acts to activate transcription (11). Clearly, the mechanistic action of FOXN3 in mammalian cells needs further elucidation.…”

Section: Introductionmentioning

confidence: 99%

“…The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer Wanjin Li, 1 Zihan Zhang, 1 Xinhua Liu, 2,3 Xiao Cheng, 1 Yi Zhang, 4 Xiao Han, 1 Yu Zhang, 1 Shumeng Liu, 1 Jianguo Yang, 1 Bosen Xu, 1 Lin He, 1 Luyang Sun, 1 Jing Liang, 1 and Yongfeng Shang dose-dependent fashion in all of the 3 reporter systems ( Figure 1A). However, overexpression of FLAG-tagged FOXN3 (FLAG-FOXN3) did not affect the activity of the Gal4-driven reporters, suggesting that FOXN3 must be physically associated with DNA to exert its repression activity ( Figure 1A).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer

Zhang

Liu

et al. 2017

Journal of Clinical Investigation

163

155

View full text Add to dashboard Cite

“…Therefore, facilitating new research in cranio-and orofacial development is paramount to prevention and treatment of these types of birth defects in humans. Xenopus laevis has emerged as a new tool for dissecting the mechanisms governing craniofacial development (some examples include 2,3,[4][5][6][7][8][9][10][11] ). Therefore, a quantitative method to analyze size and shape changes during development of the head and face of this species could be very powerful 3 .…”

Section: Introductionmentioning

confidence: 99%

Quantification of Orofacial Phenotypes in Xenopus

Kennedy

Dickinson

2014

JoVE

View full text Add to dashboard Cite

Xenopus has become an important tool for dissecting the mechanisms governing craniofacial development and defects. A method to quantify orofacial development will allow for more rigorous analysis of orofacial phenotypes upon abrogation with substances that can genetically or molecularly manipulate gene expression or protein function. Using two dimensional images of the embryonic heads, traditional size dimensionssuch as orofacial width, height and area-are measured. In addition, a roundness measure of the embryonic mouth opening is used to describe the shape of the mouth. Geometric morphometrics of these two dimensional images is also performed to provide a more sophisticated view of changes in the shape of the orofacial region. Landmarks are assigned to specific points in the orofacial region and coordinates are created. A principle component analysis is used to reduce landmark coordinates to principle components that then discriminate the treatment groups. These results are displayed as a scatter plot in which individuals with similar orofacial shapes cluster together. It is also useful to perform a discriminant function analysis, which statistically compares the positions of the landmarks between two treatment groups. This analysis is displayed on a transformation grid where changes in landmark position are viewed as vectors. A grid is superimposed on these vectors so that a warping pattern is displayed to show where significant landmark positions have changed. Shape changes in the discriminant function analysis are based on a statistical measure, and therefore can be evaluated by a p-value. This analysis is simple and accessible, requiring only a stereoscope and freeware software, and thus will be a valuable research and teaching resource. Video LinkThe video component of this article can be found at

show abstract

FoxN3 is required for craniofacial and eye development of Xenopus laevis

Cited by 52 publications

References 66 publications

Prohibitin1 acts as a neural crest specifier in Xenopus development by repressing the transcription factor E2F1

Prohibitin1 acts as a neural crest specifier in Xenopus development by repressing the transcription factor E2F1

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer

Quantification of Orofacial Phenotypes in <em>Xenopus</em>

Contact Info

Product

Resources

About