flaB
 Gene as a Molecular Marker for Distinct Identification of Borrelia Species in Environmental Samples by the PCR-Restriction Fragment Length Polymorphism Method

Wodecka, Beata

doi:10.1128/aem.05437-11

Cited by 39 publications

(39 citation statements)

References 17 publications

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“…These areas were surrounded by fields, and as shown in the study by Hubelaek et al [25] and Bartosik et al [24], the highest risk of infection with the B. burgdorferi s. l., exists in deciduous and mixed forests, whereas the lack of this pathogen in ticks from Jarnołtówek where mixed forest areas dominated, may be caused mainly by the low number of studied ticks. To-date, a total of nine B. burgdorferi s. l. genospecies have been detected in I. ricinus ticks in Poland [26]. In general, the dominant genospecies both in Poland and in Europe is B. afzelii [12].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland

Asman¹,

Witecka²,

Solarz³

et al. 2019

Ann Agric Environ Med.

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Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Abstract Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis. Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland. Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively. Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland

Asman¹,

Witecka²,

Solarz³

et al. 2019

Ann Agric Environ Med.

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“…It was first isolated from ticks in 1995 in I. persulcatus from Japan [14] and then later in other Ixodid tick species in North America [15], Canada [16] and Europe. B. miyamotoi bacteria have since been found in I. ricinus in at least eight different European countries [13,[17][18][19][20][21][22] where prevalence rates have varied between 0·5% and 5%, and tend to be tenfold less than that of B. burgdorferi s.l. [23,24].…”

Section: Introductionmentioning

confidence: 99%

Borrelia miyamotoiin host-seekingIxodes ricinusticks in England

et al. 2014

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This paper reports the first detection of Borrelia miyamotoi in UK Ixodes ricinus ticks. It also reports on the presence and infection rates of I. ricinus for a number of other tick-borne pathogens of public health importance. Ticks from seven regions in southern England were screened for B. miyamotoi, Borrelia burgdorferi sensu lato (s.l.), Anaplasma phagocytophilum and Neoehrlichia mikurensis using qPCR. A total of 954 I. ricinus ticks were tested, 40 were positive for B. burgdorferi s.l., 22 positive for A. phagocytophilum and three positive for B. miyamotoi, with no N. mikurensis detected. The three positive B. miyamotoi ticks came from three geographically distinct areas, suggesting a widespread distribution, and from two separate years, suggesting some degree of endemicity. Understanding the prevalence of Borrelia and other tick-borne pathogens in ticks is crucial for locating high-risk areas of disease transmission.

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“…afzelii and B . burgdorferi sensu stricto (s.s.) (Kurtenbach et al ., ; Wodecka, ; Franke et al ., ). These genospecies can also be found in medium‐sized mammal like hedgehogs, hares and squirrels (Gern et al ., ; Kjelland et al ., ; Pisanu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…The B. burgdorferi s.l. genospecies, which are Borrelia species discriminated genetically, found in small mammals in Europe are mainly B. afzelii and B. burgdorferi sensu stricto (s.s.) (Kurtenbach et al, 2002;Wodecka, 2011;Franke et al, 2013). These genospecies can also be found in medium-sized mammal like hedgehogs, hares and squirrels (Gern et al, 1997;Kjelland et al, 2011;Pisanu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Ecological factors influencing small mammal infection by Anaplasma phagocytophilum and Borrelia burgdorferi s.l. in agricultural and forest landscapes

Perez

Bastian

Chastagner

et al. 2017

Environmental Microbiology

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Small mammals are key components of numerous tick-borne disease systems, as hosts for immature ticks and pathogen reservoirs. To study the factors influencing tick-borne infection in small mammals, we trapped small mammals and collected questing ticks in spring and autumn in 2012 and 2013 at 24 sites in a 10 × 15 km rural landscapes (Brittany, France). Tissue samples were screened by real-time PCR for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato. Of the two dominant small mammal species captured, bank voles (Myodes glareolus) had higher prevalence than wood mice (Apodemus sylvaticus) for both infections, presumably because of specific differences in immunological defenses. Prevalence of infections was higher in 2013 than in 2012, likely because small mammals were fivefold less abundant in 2013, favouring tick aggregation. Bacterial prevalence, which was higher in autumn, was not associated to questing Ixodes ricinus nymph abundance which was six times higher in spring, but rather to the structure of the small mammal community. These findings suggest the involvement of endophilic tick species, I. trianguliceps and/or I. acuminatus, in bacterial transmission. Our study highlights that the entire community of hosts and vectors, and their interactions, should be considered to fully understand the epidemiology of vector-borne diseases.

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flaB Gene as a Molecular Marker for Distinct Identification of Borrelia Species in Environmental Samples by the PCR-Restriction Fragment Length Polymorphism Method

Cited by 39 publications

References 17 publications

Occurrence of <i>Borrelia burgdorferi</i> sensu lato, <i>Anaplasma phagocytophilum</i> and <i>Babesia microti</i> in <i>Ixodes ricinus</i> ticks collected from selected areas of Opolskie Province in south-west Poland

Occurrence of <i>Borrelia burgdorferi</i> sensu lato, <i>Anaplasma phagocytophilum</i> and <i>Babesia microti</i> in <i>Ixodes ricinus</i> ticks collected from selected areas of Opolskie Province in south-west Poland

Borrelia miyamotoiin host-seekingIxodes ricinusticks in England

Ecological factors influencing small mammal infection by Anaplasma phagocytophilum and Borrelia burgdorferi s.l. in agricultural and forest landscapes

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