<i>First Prize:</i> Ureteral Segmental Replacement Revisited

El-Hakim, Assaad; Marcovich, Robert; Chiu, Kun Yuan; Lee, Benjamin R.; Smith, Arthur D.

doi:10.1089/end.2005.19.1069

Cited by 33 publications

(17 citation statements)

References 12 publications

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“…Moreover, in case of an ureter replacement with the Optimaix 3D prototype, an additional layer of omentum or peritoneum might be used to prevent leakage. This procedure is already used for clinical applications [20] and might enhance neovascularization of tissue engineered constructs [21,22]. …”

Section: Discussionmentioning

confidence: 99%

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

Seifarth

Goßmann²,

Janke³

et al. 2015

Urol Int

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Regenerative medicine, tissue engineering and biomedical research give hope to many patients who need bio-implants. Tissue engineering applications have already been developed based on bioreactors. Physiological ureter implants, however, do not still function sufficiently, as they represent tubular hollow structures with very specific cellular structures and alignments consisting of several cell types. The aim of this study was to a develop a new bioreactor system based on seamless, collagenous, tubular OPTIMAIX 3D prototype sponge as scaffold material for ex-vivo culturing of a tissue engineered ureter replacement for future urological applications. Particular emphasis was given to a great extent to mimic the physiological environment similar to the in vivo situation of a ureter. NIH-3T3 fibroblasts, C2C12, Urotsa and primary genitourinary tract cells were applied as co-cultures on the scaffold and the penetration of cells into the collagenous material was followed. By the end of this study, the bioreactor was functioning, physiological parameter as temperature and pH and the newly developed BIOREACTOR system is applicable to tubular scaffold materials with different lengths and diameters. The automatized incubation system worked reliably. The tubular OPTIMAIX 3D sponge was a suitable scaffold material for tissue engineering purposes and co-cultivation procedures.

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Section: Discussionmentioning

confidence: 99%

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

Seifarth

Goßmann²,

Janke³

et al. 2015

Urol Int

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“…Matrici arricchite di vari tipi di cellule sono state impiegate allo scopo di migliorare la funzionalità dell'uretere. I risultati ottenuti con cellule uroteliali non sono stati confortanti; al contrario, utilizzando cellule mononucleate derivate dal midollo osseo supportate da matrici, si è ottenuta neovascolarizzazione (42,43). L'ingegneria tessutale sembra offrire delle prospettive nel trattamento dei restringimenti uretrali.…”

Section: Ricostruzione Della Vescica Dell'uretere E Dell'uretraunclassified

Stem Cells and Tissue Engineering Techniques

Sica

2013

Urologia

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The therapeutic use of stem cells and tissue engineering techniques are emerging in urology. Here, stem cell types, their differentiating potential and fundamental characteristics are illustrated. The cancer stem cell hypothesis is reported with reference to the role played by stem cells in the origin, development and progression of neoplastic lesions. In addition, recent reports of results obtained with stem cells alone or seeded in scaffolds to overcome problems of damaged urinary tract tissue are summarized. Among others, the application of these biotechnologies in urinary bladder, and urethra are delineated. Nevertheless, apart from the ethical concerns raised from the use of embryonic stem cells, a lot of questions need to be solved concerning the biology of stem cells before their widespread use in clinical trials. Further investigation is also required in tissue engineering utilizing animal models.

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“…Trials to improve functional outcome were attempted by matrix seeding with different types of cells; The results were not promising when using a urothelial seeded acellular matrix in the replacement of a 3‐cm segmental defect 32 . On the contrary, matrices enhancement with BM‐derived mononuclear cells showed neovascularization and angiogenesis 33 …”

Section: Applications Of Tissue Engineering and Stem Cells In The Genmentioning

confidence: 99%

Tissue engineering and stem cells: Basic principles and applications in urology

2010

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Abstract:To overcome problems of damaged urinary tract tissues and complications of current procedures, tissue engineering (TE) techniques and stem cell (SC) research have achieved great progress. Although diversity of techniques is used , urologists should know the basics. We carried out a literature review regarding the basic principles and applications of TE and SC technologies in the genitourinary tract. We carried out MEDLINE/PubMed searches for English articles until March 2010 using a combination of the following keywords: bladder, erectile dysfunction, kidney, prostate, Peyronie's disease, stem cells, stress urinary incontinence, testis, tissue engineering, ureter, urethra and urinary tract. Retrieved abstracts were checked , and full versions of relevant articles were obtained. Scientists have achieved great advances in basic science research. This is obvious by the tremendous increase in the number of publications. We divided this review in two topics; the first discusses basic science principles of TE and SC, whereas the second part delineates current clinical applications and advances in urological literature. TE and SC applications represent an alternative resource for treating complicated urological diseases. Despite the paucity of clinical trials, the promising results of animal models and continuous work represents the hope of treating various urological disorders with this technology.

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First Prize: Ureteral Segmental Replacement Revisited

Cited by 33 publications

References 12 publications

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

Stem Cells and Tissue Engineering Techniques

Tissue engineering and stem cells: Basic principles and applications in urology

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