<i>fbl</i> gene as a species‐specific target for <i>Staphylococcus lugdunensis</i> identification

Chatzigeorgiou, Kalliopi-Stavroula; Siafakas, Nikolaos; Petinaki, Efthymia; Zerva, Loukia

doi:10.1002/jcla.20352

Cited by 12 publications

(9 citation statements)

References 19 publications

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“…In our strain collection all 58 strains were shown to be fbl positive using a primer set targeting the A-region of the fbl gene. This prevalence is similar to the high occurrence of the fbl previously described [ 22 , 23 ]. All S. lugdunensis strains were tanA positive (100%) and all were identified as S. lugdunensis by MALDI-TOF MS (100%).…”

Section: Resultssupporting

confidence: 90%

“…Since only 94.8% of S. lugdunensis isolates were PYR positive, an identification strategy based solely on the determination of the PYR activity could miss an estimated 5% of the S. lugdunensis isolates. In addition, the fibrinogen binding gene fbl has been used to establish a S. lugdunensis species-specific PCR [ 22 , 23 ]. In our strain collection all 58 strains were shown to be fbl positive using a primer set targeting the A-region of the fbl gene.…”

Section: Resultsmentioning

confidence: 99%

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Occurrence of genes of putative fibrinogen binding proteins and hemolysins, as well as of their phenotypic correlates in isolates of S. lugdunensis of different origins

et al. 2011

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BackgroundStaphylococcus lugdunensis is an important human pathogen that causes potentially fatal endocarditis, osteomyelitis and skin and soft tissue infections similar to diseases caused by Staphylococcus aureus. Nevertheless, in contrast to S. aureus, data on pathogenicity factors of S. lugdunensis is scarce. Two adhesins, a fibrinogen and a von Willebrand factor binding protein, and a S. lugdunensis synergistic hemolysin (SLUSH) have been previously described. Moreover, the newly sequenced genome of S. lugdunensis revealed genes of other putative fibrinogen binding adhesins and hemolysins. The aim of this study was to gain more insight into the occurrence of genes likely coding for fibrinogen binding adhesins and hemolysins using clinical strains of S. lugdunensis.FindingsMost of the putative adhesin genes and hemolysin genes investigated in this study were highly prevalent, except for the SLUSH gene cluster. In contrast to previous reports, binding to fibrinogen was detected in 29.3% of the S. lugdunensis strains. In most strains, hemolysis on blood agar plates was weak after 24 h and distinct after 48 h of incubation. The fibrinogen binding and hemolysis phenotypes were also independent of the type of clinical specimen, from which the isolates were obtained.ConclusionIn this study we described a pyrrolidonyl arylamidase negative S. lugdunensis isolate. Our data indicate that a matrix-assisted laser desorption ionisation time-of-flight MS-based identification of S. lugdunensis or species-specific PCR's should be performed in favour of pyrrolidonyl arylamidase testing. In contrast to the high occurrence of putative fibrinogen binding protein genes, 29.3% of the S. lugdunensis strains bound to fibrinogen. Putative hemolysin genes were also prevalent in most of the S. lugdunensis strains, irrespective of their hemolysis activity on Columbia blood agar plates. Similar to a previous report, hemolysis after 48 h of incubation is also indicative for S. lugdunensis. The SLUSH gene cluster was detected in an estimated 50% of the strains, indicating that this locus is different or non-prevalent in many strains.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Occurrence of genes of putative fibrinogen binding proteins and hemolysins, as well as of their phenotypic correlates in isolates of S. lugdunensis of different origins

et al. 2011

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“…Fbl and ClfA have similar binding affinities to fibrinogen, and both interact with the C-terminus of the γ-chain (67). Fbl also is used as a species-specific detection method for S. lugdunensis (68, 69). …”

Section: Staphylococcal Adhesinsmentioning

confidence: 99%

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

2016

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The Staphylococci comprise a diverse genus of Gram-positive, non-motile commensal organisms that inhabit the skin and mucous membranes of humans and other mammals. In general, Staphylococci are benign members of the natural flora, but many species have the capacity to be opportunistic pathogens, mainly infecting individuals who have medical device implants or are otherwise immunocompromised. S. aureus and S. epidermidis are a major source of hospital-acquired infections and are the most common causes of surgical site infections and central line-associated bloodstream infections. The ability of Staphylococci to form biofilms in vivo makes them highly resistant to chemotherapeutics and leads to chronic diseases. These biofilm infections include osteomyelitis, endocarditis, medical device implants, and persistence in the cystic fibrosis lung. Here, we provide a comprehensive analysis of our current understanding of Staphylococcal biofilm formation, with an emphasis on adhesins and regulation, while also addressing how Staphylococcal biofilms interact with the immune system. On the whole, this review will provide a thorough picture of biofilm formation of the Staphylococcus genus and how this mode of growth impacts the host.

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“…As previously described, PCR amplifies a 239-bp fragment of tanA and a 63-bp fragment of fbl. 19,20 Antimicrobial susceptibility testing…”

Section: Genotypic Identification Of S Lugdunensismentioning

confidence: 99%

Emergence of oxacillin-resistant Staphylococcus lugdunensis carrying staphylococcal cassette chromosome mec type V in central Taiwan

Yen

Sung

Lin

et al. 2016

Journal of Microbiology, Immunology and Infection

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fbl gene as a species‐specific target for Staphylococcus lugdunensis identification

Cited by 12 publications

References 19 publications

Occurrence of genes of putative fibrinogen binding proteins and hemolysins, as well as of their phenotypic correlates in isolates of S. lugdunensis of different origins

Occurrence of genes of putative fibrinogen binding proteins and hemolysins, as well as of their phenotypic correlates in isolates of S. lugdunensis of different origins

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

Emergence of oxacillin-resistant Staphylococcus lugdunensis carrying staphylococcal cassette chromosome mec type V in central Taiwan

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