<i>Fasciola hepatica</i>: comparison of immature and mature immunoreactive glycoproteins

Dalton, John P.; Tom, Timothy D.; Strand, Mette

doi:10.1111/j.1365-3024.1985.tb00108.x

Cited by 15 publications

(8 citation statements)

References 31 publications

(7 reference statements)

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“…The humoral response is primarily of the IgG isotype and is directed against the glycocalyx of the susceptible juvenile fluke-integument (Duffus and Franks, 1981). It involves protein-bound carbohydrate epitopes (Dalton et al, 1985) and is non-protective (Bossaert et al, 2000). The effector mechanism of protective immunity, antibody-dependent cell-mediated cytotoxicity (ADCC), occurs at three sites of the juvenile fluke´s migration, i. e., the wall of the small intestine, the peritoneal cavity and the liver surface/parenchyma.…”

Section: Introductionmentioning

confidence: 99%

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

Wuhrer¹,

Berkefeld²,

Dennis³

et al. 2001

Biological Chemistry

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Glycosphingolipids from the parasitic liver flukes Fasciola gigantica and Fasciola hepatica were isolated and their carbohydrate moieties were structurally analysed by methylation analysis, exoglycosidase treatment, on-target exoglycosidase cleavage and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. For both liver fluke species, the ceramide monohexosides Gal1-ceramide and Glc1-ceramide were found in relative amounts of 1.0 to 0.1, respectively. From F. gigantica, the ceramide dihexoside was isolated in sufficient amounts to be structurally determined as lactosylceramide, Gal beta4-Glc1-ceramide, while for both liver fluke species the ceramide trihexoside was shown to be Gal alpha4Gal beta4-Glc1-ceramide, which is designated as either globotriaosylceramide, Pk-blood group antigen or CD77 leucocyte cluster of differentiation antigen. To our knowledge, this is the first report on the expression of globo-series glycosphingolipids in non-mammalian species. Ceramide analysis of ceramide monohexosides yielded as major components octadecanoic and 2-hydroxyoctadecanoic fatty acids together with C18- and C20-phytosphingosines. By the use of an anti-CD77 monoclonal antibody and the Escherichia coli Shiga toxin B1 subunit, globotriaosylceramide could be immunolocalised to the tegument of F. hepatica cryosections. The sharing of CD77 between liver flukes and their mammalian hosts fits in with the concept of molecular mimicry, which is closely parallel to the established imitation of host CD15 (Lewis X) displayed by the blood fluke Schistosoma mansoni.

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Section: Introductionmentioning

confidence: 99%

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

Wuhrer¹,

Berkefeld²,

Dennis³

et al. 2001

Biological Chemistry

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“…These molecules, which are primarily glycoconjugates, are involved in parasite survival, infectivity, host-cell recognition and immune stimulation or protection (Nyame et al, 2004). The relationship between parasite-derived glycoconjugates and host responses has been studied in many parasitic diseases such as schistosomiasis (Hokke et al, 2007), echinostomiasis (Fujino et al, 1996a,b) and fascioliasis (Dalton et al, 1985). In O. viverrini infection, secreted and cell surface tegumental components of the parasite are detected along the host biliary epithelium and activate immune/inflammatory responses (Sripa et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Stage-specific expression and antigenicity of glycoprotein glycans isolated from the human liver fluke, Opisthorchis viverrini

Talabnin

Aoki

Saichua

et al. 2013

International Journal for Parasitology

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Infection by Opisthorchis viverrini (liver fluke) is a major public health problem in southeastern Asia, resulting in hepatobiliary disease and cholangiocarcinoma. Fluke surface glycoconjugates are prominently presented to the host, thereby constituting a crucial immunological interface that can determine the parasite’s success in establishing infection. Therefore, N- and O-linked glycoprotein glycan profiles of the infective metacercarial stage and of the mature adult were investigated by nanospray ionization-linear ion trap mass spectrometry (NSI-MSn). Glycan immunogenicity was investigated by immunobloting with serum from infected humans. Metacercariae and adult parasites exhibit similar glycan diversity, although the prevalence of individual glycans and glycan classes varies by stage. The N-glycans of the metacercaria are mostly high mannose and monofucosylated, truncated-type oligosaccharides (62.7%), with the remainder processed to complex and hybrid type glycans (37.3%). The N-linked glycan profile of the adult is also dominated by high mannose and monofucosylated, truncated-type oligosaccharides (80.0%), with a smaller contribution from complex and hybrid type glycans (20.0%). At both stages, complex and hybrid type glycans are detected as mono-, bi-, tri-, or tetra-antennary structures. In metacercariae and adults, O-linked glycans are detected as mono- to pentasaccharides. The mucin type core 1 structure, Galβ1-3GalNAc, predominates in both stages but is less prevalent in the adult than in the metacercaria. Immunogenic recognition of liver fluke glycoproteins is reduced after deglycosylation but infected human serum was unable to recognize glycans released from peptides. Therefore, the most potent liver fluke antigenic epitopes are mixed determinants, comprised of glycan and polypeptide elements.

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“…This lectin is located at the tegument of these parasites and in their excretory/secretory antigens, and it is involved in immune response evasion mechanisms. Dalton et al (1985) observed high molecular weight peptides in the surface of F. hepatica flukes, which could led us to consider that the 190 and 160 kDa peptides common to the three helminths are located in their tegument. To explain their presence in the excretory/ secretory antigens, Maizels et al (1987) pointed out that excretory/secretory antigens are packaged and delivered to the surface of the worm which is metabolically active.…”

Section: Discussionmentioning

confidence: 96%

Diagnosis of Parasitic Zoonoses by Immunoenzymatic Assays—Analysis of Cross‐Reactivity Among the Excretory/Secretory Antigens ofFasciola hepatica,Toxocara canis, andAscaris suum

Romasanta

Romero

Arias

et al. 2003

Immunological Investigations

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Several parasitic infections such fasciolosis, toxocariosis or ascariosis are important zoonoses. During the infection with Fasciola hepatica, Toxocara canis and Ascaris suum, an important intraorganic phase in their hosts takes place, releasing antigens responsible for a humoral immune response, which enables the diagnosis of that parasitosis. A study to identify the existence of cross-reactivity among the excretory/ secretory antigens of F. hepatica, T. canis and A. suum was developed. One group of Sprague-Dawley rats was infected with 20 metacercariae of F. hepatica and another group remained uninfected as control. By means of an Indirect-ELISA, the rat humoral immune response (IgG and IgM) against the excretory/secretory antigens of F. hepatica was analysed and measured for cross reactivity with T. canis and A. suum. IgM cross-reaction was mainly observed in the first 10 weeks post-infection. IgG cross-reaction was observed throughout the study, and was maximal at the 2-3 weeks and 3-6 weeks post-infection, which corresponds to the intraorganic migratory phase of these parasites. The western-blot showed that the rat IgG recognised three proteins of 190, 160 and 33 kDa in the antigens from F. hepatica, T. canis and A. suum. The existence of cross-reactivity among these antigens seems to demonstrate also the presence of structural similarities, such as tegumental proteins. These results should be consider when immunoassay probes are used in the diagnosis of parasitic infections.

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Fasciola hepatica: comparison of immature and mature immunoreactive glycoproteins

Cited by 15 publications

References 31 publications

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

Stage-specific expression and antigenicity of glycoprotein glycans isolated from the human liver fluke, Opisthorchis viverrini

Diagnosis of Parasitic Zoonoses by Immunoenzymatic Assays—Analysis of Cross‐Reactivity Among the Excretory/Secretory Antigens ofFasciola hepatica,Toxocara canis, andAscaris suum

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