<i>Ex vivo</i> evaluation of the effects of several root canal preparation techniques and irrigation regimens on a mixed microbial infection

Nakamura, Vitor Cesar; Cai, Silvana; Candeiro, George Táccio de Miranda; Ferrari, Patricia Helena Pereira; Caldeira, Celso Luiz; Gavini, Giulio

doi:10.1111/j.1365-2591.2012.02110.x

Cited by 33 publications

(38 citation statements)

References 36 publications

(86 reference statements)

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“…Previous ex vivo studies have shown that manual and conventional rotary preparations have similar effects on microbial populations within straight root canals. 14,20,21 This was corroborated in the present study and might be explained by the ability of the instruments to remove contaminated dentine, ) indicate the presence of significant differences in percent reduction between the groups (p < 0.001). Lower case letters ( a or b ) indicate significant differences between the groups, before and after instrumentation, respectively (p < 0.001).…”

Section: Discussionsupporting

confidence: 78%

“…These results are in agreement with previous studies using similar methodologies. 14,26 Unlike previous studies, which assessed the effectiveness of the reciprocating technique in disinfecting root canals, the present study involved sectioning of the roots into thirds, and collection of dentin chips from the root canal walls. This approach enables the evaluation of the effects of the different preparation techniques on different portions of the root canal.…”

Section: Discussionmentioning

confidence: 99%

“…7 To overcome the above-mentioned anatomical and microbial challenges, the disinfection abilities of different substances and procedures have been studied, especially through ex vivo experiments involving E. faecalis biofilm models. 4,6,10,11,12,13,14 In addition, various materials and techniques have been introduced to improve the shaping and disinfection of the root canal system. Since 2008, two innovations including a new type of nickel-titanium (NiTi) alloy and an instrument offering reciprocating movement 15,16,17 have been studied.…”

Section: Introductionmentioning

confidence: 99%

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Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

et al. 2015

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Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals Abstract: The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

et al. 2015

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“…The plate culture method was used in this study because of its simplicity and efficiency (5,7,(8)(9)(10)(11)(13)(14)(15)(16)(17)(18)(19). Molecular methods has been highlighted because of their high sensibility to detect microorganisms, but such a feature would not be necessary in this study as there was no total absence of bacterial growth in the collection.…”

Section: Discussionmentioning

confidence: 99%

Instrument Design May Influence Bacterial Reduction During Root Canal Preparation

et al. 2017

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The aim of this study was to evaluate the bacterial reduction promoted by ProTaper Next and Twisted File by comparing to ProTaper Universal and manual technique. Sixty distobuccal root canals of maxillary molars sterilized with ethylene oxide were contaminated with Enterococcus faecalis broth culture. After incubation for 21 days, bacterial samples were collected and cultured on m-Enterococcus agar plates. The root canals were divided into 4 groups, according to the system used for instrumentation: ProTaper Next, Twisted File, ProTaper Universal, and crown down manual technique. Other 8 uncontaminated root canals were control asepsis. Bacterial samples were collected immediately and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by paired t-test and intergroup analysis by ANOVA and Tukey tests, all at 5% significance. All techniques significantly reduced the bacterial number in the root canal (p<0.05). ProTaper Next and Twisted File resulted in more bacterial reduction than ProTaper Universal and manual technique (p<0.05). ProTaper Next and Twisted File were similar (p>0.05). It can be concluded that ProTaper Next and Twisted File promote a higher bacterial reduction than Protaper Universal and manual technique.

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“…to count the number of bacteria that already inoculated into the canal to compare it with that found after irrigation of root canal with the tested irrigation A1 (Green Tea), and A2 (Propolis), and after obturation with B1 (AH Plus sealer), and B2 (Total Fill BC Sealer), (19) on the number of inoculated bacteria (20) .…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of the Antibacterial Efficacy of two Natural Irrigating Solutions with Two Different Root Canal Sealers on E-feacalis

et al. 2017

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This study aimed to: evaluate the antibacterial efficacy of two natural irrigating solutions (Green Tea, and Propolis) with two different root canal sealers (AH Plus sealer, and Total Fill BC Sealer) against E. faecalis. Methods: One hundred single rooted teeth were selected. All root canals were prepared manually to #50 file. Finally the teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E.faecalis. Specimens were divided randomly into two main groups (50 specimens each) according to the irrigating solution; A1 (Green Tea), and A2 (Propolis). Each main group was divided into two subgroups with (25 specimens each) according to the type of sealer used B1 (AH Plus sealer), and B2 (TotalFill BC Sealer). The bacterial sampling counting process was done three times throughout the study. Five specimens from each subgroup were examined by SEM for presence or absence of E. faecalis. Results:The highest mean value of bacterial count was found in (Green tea) (6.50x10x10 ± 1.29x10x10) while the least mean value of bacterial count was found in (Propolis) (with less antibacterial effect 2x10x10 ± 1.33x10x10) with higher antibacterial effect. The highest mean value of bacterial count was found in (AH Plus Sealer) (5.40x10x10 ± 2.44x10x10) with less antibacterial effect while the least mean value of bacterial count was found in (TotalFill BC Sealer) (3.10x10x10 ± 1.54 x10x10) with higher antibacterial effect. Conclusions: Propolis is an effective intracanal irrigant in eradicating E.faecalis. On other hand TotalFill BC Sealer is more effective in reducing E. faecalis.

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Ex vivo evaluation of the effects of several root canal preparation techniques and irrigation regimens on a mixed microbial infection

Abstract: Irrigation with 5.25% NaOCl and 1% NaOCl alternated with 15% citric acid reduced microorganisms in infected root canals significantly more than saline and 1% NaOCl.

Cited by 33 publications

References 36 publications

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

Instrument Design May Influence Bacterial Reduction During Root Canal Preparation

A Comparative Study of the Antibacterial Efficacy of two Natural Irrigating Solutions with Two Different Root Canal Sealers on E-feacalis

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