Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

Abstract

Double-stranded RNA (dsRNA) is a pathogen-associated molecular pattern, known for its ability to induce antiviral response and enhance communication between cells mediating innate and adaptive immune responses. The aim of this study was to characterize the effect of the dsRNA-containing product Larifan on the production of a wide spectrum of cytokines and chemokines in ex vivo cultivated peripheral blood mononuclear cells. Concentrations of 29 different cytokines were detected by a Luminex® 200™ System using three Milliplex MAP Multiplex Assay Kits. Larifan caused strong induction of chemokine macrophage inflammatory protein 1β, I-309, and TARC, proinflammatory cytokines IL-6, tumor necrosis factor -α, granulocyte macrophage colony-stimulating factor, anti-inflammatory IL-10, and cellular immunity mediating factors IL-23 and interferon-γ. Considerable suppression of IL-16 and chemokine stromal cell-derived factor 1 a+b and interferon gamma-induced protein 10 was also observed. The network of molecules responding to the presence of Larifan revealed the pleiotropic effect this product exerts on immune response.

Modulators affecting the immune dialogue between human immune and colon cancer cells

“…This particular function is promoted by various factors, such as amorphous silica particles capable to increase IL-1β and IL-8 production [25] , and cortisol that have been shown to suppress secretion of IL-1β, IL-6, IL-17 and G-CSF [26] . Recently, Veinalde et al [27] have reported that double stranded RNA has the capacity to induce PBMC to produce a considerable number of both pro-and antiinflammatory cytokines. Metal particles, such as titanium and stainless steel originating in patients with implanted medical devices have been shown to enhance increased production of IL-6 and IL-1 by PBMC, but to lower the level of TNF-α [28] .…”

Biotechnological and environmental microbiological research in the Baltic region

“…Larifan also has a potential application in oncology, as it has exhibited an antitumoral effect in several preliminary experimental clinical investigations. The results obtained by this group and described in the paper revealed the pleiotropic effect of Larifan on the immune response.…”

“…A biotechnological study of Latvian researchers published in this issue is devoted to the characterization of the effect of dsRNA‐containing product Larifan on the production of a wide spectrum of cytokines, which are secreted proteins that possess the ability to modulate immune response, and chemokines whose key function is to traffic various subsets of lymphocytes. Larifan is a pharmaceutical product obtained from Escherichia coli cells infected with a bacteriophage .…”

The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma

“…The preparation has an interferonogenic and immunomodu latory properties. It exhibited an antitumoral effect in the investigations with experimental tumor models [17,18].…”