<i>Ex vivo</i>
                    characterization of a novel tissue-like cross-linked fibrin-agarose hydrogel for tissue engineering applications

Campos, Fernando; Bonhome-Espinosa, Ana B.; García-Martínez, Laura; Durán, J.D.G.; López–López, Modesto T.; Alaminos, Miguel; Sánchez-Quevedo, M.C.; Carriel, Víctor

doi:10.1088/1748-6041/11/5/055004

Cited by 38 publications

(58 citation statements)

References 38 publications

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“…The experiments were [ 42 , 43 ]: (i) amplitude sweep: frequency was kept constant at 1, 2, 5, 10 and 20 Hz, and shear amplitude was varied up to values in which nonlinear behavior was reached. Nonlinearity was developed and was clearly distinguishable when

and

were not only functions of

but also strongly depended on

.…”

Section: Methodsmentioning

confidence: 99%

Performance Study of a Torsional Wave Sensor and Cervical Tissue Characterization

Callejas

Gómez

Melchor

et al. 2017

Sensors

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A novel torsional wave sensor designed to characterize mechanical properties of soft tissues is presented in this work. Elastography is a widely used technique since the 1990s to map tissue stiffness. Moreover, quantitative elastography uses the velocity of shear waves to achieve the shear stiffness. This technique exhibits significant limitations caused by the difficulty of the separation between longitudinal and shear waves and the pressure applied while measuring. To overcome these drawbacks, the proposed torsional wave sensor can isolate a pure shear wave, avoiding the possibility of multiple wave interference. It comprises a rotational actuator disk and a piezoceramic receiver ring circumferentially aligned. Both allow the transmission of shear waves that interact with the tissue before being received. Experimental tests are performed using tissue mimicking phantoms and cervical tissues. One contribution is a sensor sensitivity study that has been conducted to evaluate the robustness of the new proposed torsional wave elastography (TWE) technique. The variables object of the study are both the applied pressure and the angle of incidence sensor–phantom. The other contribution consists of a cervical tissue characterization. To this end, three rheological models have fit the experimental data and a static independent testing method has been performed. The proposed methodology permits the reconstruction of the mechanical constants from the propagated shear wave, providing a proof of principle and warranting further studies to confirm the validity of the results.

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and

were not only functions of

but also strongly depended on

.…”

Section: Methodsmentioning

confidence: 99%

Performance Study of a Torsional Wave Sensor and Cervical Tissue Characterization

Callejas

Gómez

Melchor

et al. 2017

Sensors

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show abstract

“…In vitro SCTLAS included in this study were processed for scanning electron microscopy (SEM) as previously described . Briefly, samples were rinsed in distilled water, dried on paper and immediately immersed in liquid nitrogen and kept at −196 °C until processing.…”

Section: Methodsmentioning

confidence: 99%

Generation and Evaluation of Novel Stromal Cell‐Containing Tissue Engineered Artificial Stromas for the Surgical Repair of Abdominal Defects

Martín-Piedra

Garzón

Gómez-Sotelo

et al. 2017

Biotechnology Journal

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Repair of abdominal wall defects is one of the major clinical challenges in abdominal surgery. Most biomaterials are associated to infection and severe complications, making necessary safer and more biocompatible approaches. In the present work, the adequate mechanical properties of synthetic polymer meshes with tissue-engineered matrices containing stromal mesenchymal cells is combined to generate a novel cell-containing tissue-like artificial stroma (SCTLAS) for use in abdominal wall repair. SCTLAS consisting on fibrin-agarose hydrogels seeded with stromal cells and reinforced with commercial surgical meshes (SM) are evaluated in vitro and in vivo in animal models of abdominal wall defect. Inflammatory cells, collagen, and extracellular matrix (ECM) components are analyzed and compared with grafted SM. Use of SCTLAS results in less inflammation and less fibrosis than SM, with most ECM components being very similar to control abdominal wall tissues. Cell migration and ECM remodeling within SCTLAS is comparable to control tissues. The use of SCTLAS could contribute to reduce the side-effects associated to currently available SM and regenerated tissues are more similar to control abdominal wall tissues. Bioengineered SCTLAS could contribute to a safer treatment of abdominal wall defects with higher biocompatibility than currently available SM.

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“…Once primary cell cultures of human skin keratinocytes and fibroblasts were obtained, bioengineered tissues were generated using fibrin-agarose biomaterials following previously published methods. 10,[23][24][25][26][27] Briefly, human plasma was used as a source of fibrin and 0.1% agarose, tranexamic acid, and calcium chloride were added and immediately aliquoted on culture plates. Four types of samples were used in this study: (1) acellular fibrin-agarose substitutes (AS), in which fibrin-agarose biomaterials were generated without cells; (2) dermal skin (DS) substitutes consisting of 800,000 human skin fibroblasts immersed within fibrin-agarose biomaterials;…”

Section: Generation Of Fibrin-agarose Hydrogelsmentioning

confidence: 99%

Evaluation of the optical and biomechanical properties of bioengineered human skin generated with fibrin-agarose biomaterials

et al. 2020

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Significance: Recent generation of bioengineered human skin allowed the efficient treatment of patients with severe skin defects. However, the optical and biomechanical properties of these models are not known. Aim: Three models of bioengineered human skin based on fibrin-agarose biomaterials (acellular, dermal skin substitutes, and complete dermoepidermal skin substitutes) were generated and analyzed. Approach: Optical and biomechanical properties of these artificial human skin substitutes were investigated using the inverse adding-doubling method and tensile tests, respectively. Results: The analysis of the optical properties revealed that the model that most resembled the optical behavior of the native human skin in terms of absorption and scattering properties was the dermoepidermal human skin substitutes after 7 to 14 days in culture. The time-course evaluation of the biomechanical parameters showed that the dermoepidermal substitutes displayed significant higher values than acellular and dermal skin substitutes for all parameters analyzed and did not differ from the control skin for traction deformation, stress, and strain at fracture break. Conclusions: We demonstrate the crucial role of the cells from a physical point of view, confirming that a bioengineered dermoepidermal human skin substitute based on fibrin-agarose biomaterials is able to fulfill the minimal requirements for skin transplants for future clinical use at early stages of in vitro development.

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Ex vivo characterization of a novel tissue-like cross-linked fibrin-agarose hydrogel for tissue engineering applications

Cited by 38 publications

References 38 publications

Performance Study of a Torsional Wave Sensor and Cervical Tissue Characterization

Performance Study of a Torsional Wave Sensor and Cervical Tissue Characterization

Generation and Evaluation of Novel Stromal Cell‐Containing Tissue Engineered Artificial Stromas for the Surgical Repair of Abdominal Defects

Evaluation of the optical and biomechanical properties of bioengineered human skin generated with fibrin-agarose biomaterials

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