<i>Ex Situ</i>Collection of Model Resurrection Plant<i>Haberlea Rhodopensis</i>as a Prerequisite for Biodiversity and Conservation Studies

Petrova, Galya; Tosheva, Anita; Mladenov, Petko; Moyankova, Daniela; Djilianov, Dimitar

doi:10.2478/v10133-010-0070-y

Cited by 9 publications

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“…2). This trait is confirmed to be stable when such plants are potted and exposed to soil drying under controlled or natural conditions (Petrova et al. 2010).…”

Section: Resultsmentioning

confidence: 75%

Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non‐resurrection species Chirita eberhardtii

Djilianov

Ivanov²,

Moyankova

et al. 2011

Plant Biology

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Because of their unique tolerance to desiccation, the so-called resurrection plants can be considered as excellent models for extensive research on plant reactions to environmental stresses. The vegetative tissues of these species are able to withstand long dry periods and to recover very rapidly upon re-watering. This study follows the dynamics of key components involved in leaf tissue antioxidant systems under desiccation in the resurrection plant Haberlea rhodopensis and the related non-resurrection species Chirita eberhardtii. In H. rhodopensis these parameters were also followed during recovery after full drying. A well-defined test system was developed to characterise the different responses of the two species under drought stress. Results show that levels of H₂O₂ decreased significantly both in H. rhodopensis and C. eberhardtii, but that accumulation of malondialdehyde was much more pronounced in the desiccation-tolerant H. rhodopensis than in the non-resurrection C. eberhardtii. A putative protective role could be attributed to accumulation of total phenols in H. rhodopensis during the late stages of drying. The total glutathione concentration and GSSG/GSH ratio increased upon complete dehydration of H. rhodopensis. Our data on soluble sugars suggest that sugar ratios might be important for plant desiccation tolerance. An array of different adaptations could thus be responsible for the resurrection phenotype of H. rhodopensis.

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“…2). This trait is confirmed to be stable when such plants are potted and exposed to soil drying under controlled or natural conditions (Petrova et al. 2010).…”

Section: Resultsmentioning

confidence: 75%

Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non‐resurrection species Chirita eberhardtii

Djilianov

Ivanov²,

Moyankova

et al. 2011

Plant Biology

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“…Gesneriaceae include several resurrection plants, typically occurring in arid regions, which have evolved unique mechanisms of desiccation tolerance (Mitra et al 2013). Bulgaria is among the few countries in Europe where two resurrection plants of Gesneriaceae, R. serbica and H. rhodopensis, share similar habitats (Petrova et al 2010). Haberlea rhodopensis is an example of a species of Tertiary origin, and persistence and survival in paleorefugia on the eastern Balkan Peninsula.…”

Section: Introductionmentioning

confidence: 99%

Low genetic diversity in small leading edge populations of the European paleoendemic Ramonda serbica (Gesneriaceae) in Bulgaria

Petrova

Petrov

Möller

2018

Nordic Journal of Botany

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Ramonda serbica Panč. (Gesneriaceae) is an endangered endemic species of the Balkan Peninsula which has been the subject of several studies in the past, but has not yet been investigated in terms of its genetic variation. Ramonda serbica is one of only five European representatives of the mainly subtropical family Gesneriaceae categorized as paleoendemics or Tertiary relicts sequestered in southern Europe by Quaternary climate oscillations. Here, an inter simple sequence repeat (ISSR) analysis was performed to determine the genetic diversity of five populations sampled from the geographically eastern fringe of its range in northwestern Bulgaria. We found relatively low levels of genetic diversity and significant genetic differentiation among the investigated populations, typical of a leading edge scenario. From a conservation point of view, the low genetic diversity, together with the presence of only few extant localities stress the need for urgent in situ and ex situ conservation actions to ensure the long‐term survival of R. serbica in Bulgaria.

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“…A voucher specimen (№ 20090600) was deposited at the Medical University of Sofia. The caffeoyl phenylethanoid glucosides (1-2) and flavone 8-C-glycosides (3)(4)(5)(6) were isolated and identified [7d]. , and (C) MeOH.…”

Section: Plant Materials and Standardsmentioning

confidence: 99%

“…Haberlea rhodopensis, family Gesneriaceae, is highly desiccationtolerant and able to revive upon re-hydration of vegetative tissues, even after prolonged periods of dehydration. The plant is used in folk medicine for treatment of wounds and diseases of stock in the Rhodope region of Bulgaria [1][2][3]. Previous investigation revealed the presence of the caffeoyl phenylethanoid glucosides myconoside and paucifloside [4,5] and flavone C-glycosides [6a].…”

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confidence: 99%

“…However, these methods are not applicable for the simultaneous analysis of the caffeoyl phenylethanoid glucosides and flavone 8-C-glycosides isolated from H. rhodopensis. The present paper describes a HPLC method for the determination and quantification of the main compounds (1)(2)(3)(4)(5)(6) in H. rhodopensis that are responsible for its pharmacological activity.…”

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confidence: 99%

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A Validated HPLC Method for Simultaneous Determination of Caffeoyl Phenylethanoid Glucosides and Flavone 8-C-glycosides in Haberlea rhodopensis

Zheleva‐Dimitrova

Nedialkov

Giresser

2016

Natural Product Communications

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A HPLC-UV method for analysis of the main compounds: caffeoyl phenylethanoid glucosides myconoside (1) and paucifloside (2) and flavone 8-C-glycosides: hispidulin 8-C-β-galactopyranoside (3), hispidulin 8-C-(2"-O-syringoyl-β-glucopyranoside) (4), hispidulin 8-C-(6-O-acetyl-βglucopyranoside) (5) and hispidulin 8-C-(6-O-acetyl-2"-O-syringoyl-β-glucopyranoside) (6) in Haberlea rhodopensis leaves was developed and validated. Compound 3 was isolated for the first time from the title species. Ultrasound extraction with 80% methanol at room temperature allowed a good recovery of analytes (from 87.2 % for 1 to 109.8 % for 3) and the precision of the entire procedure was between 1.6% and 6.9%. The subsequent HPLC separation and quantification was achieved using a Hypersil ODS C18 column and UV detection at 280 nm. The mobile phase comprised methanol and 0.1 % o-phosphoric acid, and gradient elution mode was applied. The detection limits ranged from 0.042 µg/mL (6) to 0.18 µg/mL (5). The total amount in leaves of the assayed phenolic compounds was 374.2 mg/g. Myconoside was found to be the dominant compound in H. rhodopensis extract (332.2 ± 0.7 mg/g dw) and reached up to 88.8% of the analyzed mixture in leaves, while the total content of flavone C-glycosides was 17.1 mg/g dw.

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Ex SituCollection of Model Resurrection PlantHaberlea Rhodopensisas a Prerequisite for Biodiversity and Conservation Studies

Cited by 9 publications

References 9 publications

Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non‐resurrection species Chirita eberhardtii

Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non‐resurrection species Chirita eberhardtii

Low genetic diversity in small leading edge populations of the European paleoendemic Ramonda serbica (Gesneriaceae) in Bulgaria

A Validated HPLC Method for Simultaneous Determination of Caffeoyl Phenylethanoid Glucosides and Flavone 8-C-glycosides in Haberlea rhodopensis

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