Eucommia Bark/Leaf Extract Improves Lipid Metabolism Disorders by Affecting Intestinal Microbiota and Microbiome–Host Interaction in HFD Mice

Abstract

Eucommia bark contains many bioactive compounds and has anti-hyperlipidemic effects. However, due to the slow growth rate of the plant, there is a limited supply of this resource. Studies have demonstrated that Eucommia leaves contain active ingredients similar to those of Eucommia bark and also have anti-hyperlipidemic effects. It is not currently clear whether Eucommia leaf can be used as a substitute for Eucommia bark. Furthermore, their mechanism of action for anti-hyperlipidemia by improving the structure of the gut microbiota is also unclear. We aimed to determine the composition of the active ingredients in EBE and ELE by HPLC, establish an HFD-induced hyperlipidemia model, and combine fecal microbiota transplantation (FMT) experiments to investigate the mechanism of EBE/ELE anti-hyperlipidemia by modifying the structure of intestinal microbiota, as well as to compare the effects of EBE and ELE. Our results showed that EBE and ELE contained similar active ingredients and significantly alleviated lipid metabolism disorders and blood glucose levels in the HFD-induced hyperlipidemia model. In this study, EBE and ELE significantly reduced the relative abundance of Desulfovibrionaceae and Erysipelotrichaceae and significantly increased the relative abundance of Ruminococcaceae. They also promoted the production of short-chain fatty acids (SCFAs) and activated the gene expression of the SCFA receptors G protein-coupled receptor 41 (GPR41) and GPR43. In addition, EBE and ELE can significantly increase the expression of the fasting-induced adipose factor (Fiaf) gene in the colon and inhibit the secretion of lipoprotein lipase (LPL) in the liver, thereby inhibiting triglyceride (TG) synthesis. They also significantly activate the expression of GPR41 and GPR43 genes in the epididymal fat tissue, leading to reduced lipid accumulation in adipocytes. These effects on the target genes were associated with changes in the abundance of Desulfovibrionaceae, Erysipelotrichaceae, and Ruminococcaceae bacteria in the intestinal microbiota. Thus, regulating the relative abundance of these microbes may serve as prospective targets for EBE/ELE to influence the Fiaf-LPL gut-liver axis and the SCFAs-GPR41/GPR43 gut-fat axis. In addition, there was no significant difference in the antihyperlipidemic effects of ELE and EBE, suggesting that Eucommia leaf may be a suitable alternative to Eucommia bark for managing hyperlipidemia by regulating the structure of the intestinal microbiota. These findings suggest that Eucommia leaves have great potential for development as a functional food with lipid-lowering properties.

Effects of Dietary Eucommia ulmoides Leaf Extract Supplementation on Growth Performance, Meat Quality, Antioxidant Capacity, and Lipid Metabolism of Finishing Pigs

“…ELE has been widely reported to have a lipid-lowering function [ 18 , 19 , 79 , 80 ]. Our previous work also showed that ELE could reduce fat deposition on the back of finishing pigs [ 23 ].…”

Study on chemical composition analysis and lipid metabolism regulation activity of different parts of Eucommia ulmoides

“…Preliminary laboratory studies have shown that EBE and ELE contain eight identical bioactive ingredients, and fecal bacteria suspensions containing EBE and ELE signi cantly improved HFD-induced lipid metabolism disorders and elevated blood glucose levels 34 . The results showed that EBE and ELE contained similar active ingredients and signi cantly alleviated lipid metabolism disorders and blood glucose levels in the HFD-induced hyperlipidemia model 35 .…”

Effects of plant natural products on metabolic-associated fatty liver disease and the underlying mechanisms: a narrative review with a focus on the modulation of the gut microbiota

“…Eucommia, isolated from the dry roots of Eucommia ulmoides Oliv., is a Chinese medicine and food homolog that has antiosteoporotic, anti-inflammatory, hypoglycemic and hypolipidemic effects. Recent studies have indicated that Eucommia leaves have similar active ingredients and antihyperlipidemic effects as Eucommia bark and can modulate the gut microbiota to alleviate lipid metabolism disorders ( Wang et al., 2023a ; Wang et al., 2023b ). Eucommia leaf extract supplementation increased the relative abundance of Ruminococcaceae to promote the production of butyric acid, which upregulated the expression of GPR43 to reduce the area of adipocytes and lipid accumulation.…”