<i>ETR1</i>-Specific Mutations Distinguish ETR1 from Other Arabidopsis Ethylene Receptors as Revealed by Genetic Interaction with<i>RTE1</i>

Rivarola, Máximo Lisandro; McClellan, Christopher A.; Resnick, Josephine S.; Chang, Caren

doi:10.1104/pp.109.138461

Cited by 35 publications

(26 citation statements)

References 25 publications

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“…Furthermore, in tissues where overexpression of either SlGR or SlGRL1 fails to give rise to an ethylene-insensitive phenotype, it is possible that the appropriate target receptor may be absent, present at reduced levels compared with other receptors, or that the response is mediated by receptors that do not require GR/RTE1 family proteins. The latter hypothesis is supported by the observation that the etr1-9/ers1-3 double null mutant of Arabidopsis remains responsive to ethylene, suggesting that certain ethylene responses are therefore likely to be independent of AtRTE1, given that AtRTE1 acts predominantly through the ETR1 receptor (Resnick et al, 2006;Qu et al, 2007;Zhou et al, 2007;Rivarola et al, 2009).…”

Section: Evidence For the Existence Of Distinct Ethylene Signaling Mosupporting

confidence: 53%

Differential Control of Ethylene Responses byGREEN-RIPEandGREEN-RIPE LIKE1Provides Evidence for Distinct Ethylene Signaling Modules in Tomato

Brandizzí

et al. 2012

Plant Physiology

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The factors that mediate specific responses to the plant hormone ethylene are not fully defined. In particular, it is not known how signaling at the receptor complex can control distinct subsets of ethylene responses. Mutations at the Green-ripe (Gr) and reversion to ethylene sensitivity1 (rte1) loci, which encode homologous proteins of unknown function, influence ethylene responses in tomato (Solanum lycopersicum) and Arabidopsis (Arabidopsis thaliana), respectively. In Arabidopsis, AtRTE1 is required for function of the ETR1 ethylene receptor and acts predominantly through this receptor via direct protein-protein interaction. While most eudicot families including the Brassicaceae possess a single gene that is closely related to AtRTE1, we report that members of the Solanaceae family contain two phylogenetically distinct genes defined by GR and GREEN-RIPE LIKE1 (GRL1), creating the possibility of subfunctionalization. We also show that SlGR and SlGRL1 are differentially expressed in tomato tissues and encode proteins predominantly localized to the Golgi. A combination of overexpression in tomato and complementation of the rte1-3 mutant allele indicates that SlGR and SlGRL1 influence distinct but overlapping ethylene responses. Overexpression of SlGRL1 in the Gr mutant background provides evidence for the existence of different ethylene signaling modules in tomato that are influenced by GR, GRL1, or both. In addition, overexpression of AtRTE1 in tomato leads to reduced ethylene responsiveness in a subset of tissues but does not mimic the Gr mutant phenotype. Together, these data reveal species-specific heterogeneity in the control of ethylene responses mediated by members of the GR/RTE1 family.The gaseous plant hormone ethylene influences many aspects of plant development and mediates responses to biotic and abiotic stresses (Abeles et al., 1992). Impaired ethylene biosynthesis and responsiveness can lead to altered cell expansion, cell elongation, senescence, abscission, cell death, fruit ripening, root hair formation, lateral root development, nodulation, and sex determination (Bleecker et al

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Section: Evidence For the Existence Of Distinct Ethylene Signaling Mosupporting

confidence: 53%

Differential Control of Ethylene Responses byGREEN-RIPEandGREEN-RIPE LIKE1Provides Evidence for Distinct Ethylene Signaling Modules in Tomato

Brandizzí

et al. 2012

Plant Physiology

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“…Recently it was found that REVERSION TO SENSI-TIVITY1 (RTE1) specifically interacts with ETR1 to modulate ethylene responses in Arabidopsis as a positive regulator of ETR1, but does not regulate the other receptor isoforms (Resnick et al, 2006;Zhou et al, 2007;Rivarola et al, 2009;Dong et al, 2010). Interestingly, rte1 null mutants display phenotypes similar to etr1 null mutants including reduced growth in air, ethylene hypersensitivity, and enhanced growth inhibition to application of ethylene (Resnick et al, 2006).…”

Section: Reversion To Sensitivity1 Modulates Ethylene-stimulated Nutamentioning

confidence: 99%

Ethylene Receptor ETHYLENE RECEPTOR1 Domain Requirements for Ethylene Responses in Arabidopsis Seedlings

Kim

Helmbrecht²,

Stalans³

et al. 2011

Plant Physiology

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Ethylene influences many processes in Arabidopsis (Arabidopsis thaliana) through the action of five receptor isoforms. We used high-resolution, time-lapse imaging of dark-grown Arabidopsis seedlings to better understand the roles of each isoform in the regulation of growth in air, ethylene-stimulated nutations, and growth recovery after ethylene removal. We found that ETHYLENE RECEPTOR1 (ETR1) is both necessary and sufficient for nutations. Transgene constructs in which the ETR1 promoter was used to drive expression of cDNAs for each of the five receptor isoforms were transferred into etr1-6;etr2-3;ein4-4 triple loss-of-function mutants that have constitutive growth inhibition in air, fail to nutate in ethylene, and take longer to recover a normal growth rate when ethylene is removed. The patterns of rescue show that ETR1, ETR2, and ETHYLENE INSENSITIVE4 (EIN4) have the prominent roles in rapid growth recovery after removal of ethylene whereas ETR1 was the sole isoform that rescued nutations. ETR1 histidine kinase activity and phosphotransfer through the receiver domain are not required to rescue nutations. However, REVERSION TO SENSITIVITY1 modulates ethylene-stimulated nutations but does not modulate the rate of growth recovery after ethylene removal. Several chimeric receptor transgene constructs where domains of EIN4 were swapped into ETR1 were also introduced into the triple mutant. The pattern of phenotype rescue by the chimeric receptors used in this study supports a model where a receptor with a receiver domain is required for normal growth recovery and that nutations specifically require the full-length ETR1 receptor.

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“…Although the biochemical function of RTE1 is unknown, its action in ethylene signaling seems to involve a physical interaction with ETR1 (Dong et al, 2010) and requires the N-terminal domain of ETR1 (Zhou et al, 2007;Qiu et al, 2012). Interestingly, RTE1 appears to have little or no effect on the other Arabidopsis ethylene receptors (Resnick et al, 2006;Rivarola et al, 2009), but the basis for this specificity is unknown. Expression of the RTE1 gene is somehow regulated by HYPER RECOMBINATION1 (HPR1), a component of the THO/Transcription Export complex that is related to mRNA processing (Xu et al, 2015).…”

Section: How Do the Ethylene Receptors Signal?mentioning

confidence: 99%

Mechanistic Insights in Ethylene Perception and Signal Transduction

2015

Self Cite

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The gaseous hormone ethylene profoundly affects plant growth, development, and stress responses. Ethylene perception occurs at the endoplasmic reticulum membrane, and signal transduction leads to a transcriptional cascade that initiates diverse responses, often in conjunction with other signals. Recent findings provide a more complete picture of the components and mechanisms in ethylene signaling, now rendering a more dynamic view of this conserved pathway. This includes newly identified protein-protein interactions at the endoplasmic reticulum membrane, as well as the major discoveries that the central regulator ETHYLENE INSENSITIVE2 (EIN2) is the long-sought phosphorylation substrate for the CONSTITUTIVE RESPONSE1 protein kinase, and that cleavage of EIN2 transmits the signal to the nucleus. In the nucleus, hundreds of potential gene targets of the EIN3 master transcription factor have been identified and found to be induced in transcriptional waves, and transcriptional coregulation has been shown to be a mechanism of ethylene cross talk.

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ETR1-Specific Mutations Distinguish ETR1 from Other Arabidopsis Ethylene Receptors as Revealed by Genetic Interaction withRTE1

Cited by 35 publications

References 25 publications

Differential Control of Ethylene Responses byGREEN-RIPEandGREEN-RIPE LIKE1Provides Evidence for Distinct Ethylene Signaling Modules in Tomato

Differential Control of Ethylene Responses byGREEN-RIPEandGREEN-RIPE LIKE1Provides Evidence for Distinct Ethylene Signaling Modules in Tomato

Ethylene Receptor ETHYLENE RECEPTOR1 Domain Requirements for Ethylene Responses in Arabidopsis Seedlings

Mechanistic Insights in Ethylene Perception and Signal Transduction

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