<i>Escherichia coli</i>
            RcsA, a Positive Activator of Colanic Acid Capsular Polysaccharide Synthesis, Functions To Activate Its Own Expression

SummaryGenes rcsC and rcsB form a two-component system in which rcsC encodes the sensor element and rcsB the regulator. In Escherichia coli, the system positively regulates the expression of the capsule operon, cps, and of the cell division gene ftsZ. We report the identi®cation of the promoter and of the sequences required for rcsB-dependent stimulation of ftsZ expression. The promoter, ftsA1p, located in the ftsQ coding sequence, co-regulates ftsA and ftsZ. The sequences required for rcsB activity are immediately adjacent to this promoter.

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“…5; Wehland et al, 1999). Ebel and Trempy (1999) reported that E. coli RcsA activates its own expression. This activation is potentiated by RcsB.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two‐component system rcsC–rcsB

Carballès

Bertrand

Bouché

et al. 1999

Molecular Microbiology

109

120

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“…Besides autoactivation (Ebel and Trempy, 1999), rcsA was activated by GadE (Hommais et al, 2004) and gadE was one of the 48 genes controlled by RpoS (Patten et al, 2004; Table 1). The crucial DNA-binding regulator for Rcs regulon is RcsA together with RcsB (Ebel and Trempy, 1999). Although rcsB did not change, the expression of rcsA showed a 3.5-fold increase during carbon limitation.…”

Section: Uncovering Gene Regulation Strategy At a Genomewide Scalementioning

confidence: 99%

Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation

Pan

Xiao

et al. 2018

Microbial Biotechnology

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Summary Despite decades of studies meant to analyse the bacterial response to carbon limitation, we still miss a high‐resolution overview of the situation. All gene expression changes observed in such conditions cannot solely be accounted for by the global regulator Crp either free or bound to its effector, cyclic AMP . Here, for the first time, we evaluated the response of both CDS (protein‐coding sequence) and nc RNA (non‐coding RNA ) genes to carbon limitation, revealed cellular functions of differentially expressed genes systematically, quantified the contribution of Crp‐ cAMP and other factors to regulation and deciphered regulation strategies at a genomewide scale. Approximately one‐third of the differentially expressed genes we identified responded to Crp‐ cAMP via its direct or indirect control, while the remaining genes were subject to growth rate‐dependent control or were controlled by other regulators, especially RpoS. Importantly, gene regulation mechanisms can be established by expression pattern studies. Here, we propose a comprehensive picture of how cells respond to carbon scarcity. The global regulation strategies thus exposed illustrate that the response of cell to carbon scarcity is not limited to maintaining sufficient carbon metabolism via cAMP signalling while the main response is to adjust metabolism to cope with a slow growth rate.

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“…Our results suggest that H-NS could initiate a new kind of regulation by specifically modulating the expression level of those regulators. Moreover, the effect of H-NS on genes of the colanic acid operon could be mediated by the increased expression of their activator-encoding gene, rcsA (Ebel and Trempy, 1999), in the hns mutant (Table 1). This suggests that H-NS plays an indirect role in the regulation of many genes identified in this study and further supports a high position of H-NS in the regulatory hierarchy of bacterial physiology.…”

Section: Percentage Survival Bmentioning

confidence: 99%

Large‐scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid‐associated protein, H‐NS

Hommais

Krin

Laurent‐Winter

et al. 2001

Molecular Microbiology

370

375

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Despite many years of intense work investigating the function of nucleoid‐associated proteins in prokaryotes, their role in bacterial physiology remains largely unknown. The two‐dimensional protein patterns were compared and expression profiling was carried out on H‐NS‐deficient and wild‐type strains of Escherichia coli K‐12. The expression of approximately 5% of the genes and/or the accumulation of their protein was directly or indirectly altered in the hns mutant strain. About one‐fifth of these genes encode proteins that are involved in transcription or translation and one‐third are known to or were in silico predicted to encode cell envelope components or proteins that are usually involved in bacterial adaptation to changes in environmental conditions. The increased expression of several genes in the mutant resulted in a better ability of this strain to survive at low pH and high osmolarity than the wild‐type strain. In particular, the putative regulator, YhiX, plays a central role in the H‐NS control of genes required in the glutamate‐dependent acid stress response. These results suggest that there is a strong relationship between the H‐NS regulon and the maintenance of intracellular homeostasis.

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Escherichia coli RcsA, a Positive Activator of Colanic Acid Capsular Polysaccharide Synthesis, Functions To Activate Its Own Expression

Cited by 81 publications

References 30 publications

Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two‐component system rcsC–rcsB

Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two‐component system rcsC–rcsB

Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation

Large‐scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid‐associated protein, H‐NS

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