Escherichia coli from clinical mastitis

Abstract

Abstract. In the current study, the virulence factors in Escherichia coli isolates from bovine mastitis were investigated, and the connection between these factors and infection was evaluated using phenotypic and genotypic analyses. Twenty-seven E. coli isolates were analyzed, and 2 were shown to produce verotoxin. All isolates had the ability to produce biofilms, although at different levels. One isolate was found to be sensitive to the bactericidal activity of bovine serum, 11 were intermediate, and 15 were resistant. Some isolates showed resistance to trimethoprim sulfa (9) and ampicillin (4), intermediate resistance to neomycin (1) and trimethoprim sulfa (5), and simultaneous resistance to ampicillin and trimethoprim sulfa (4). The fimH gene was found in all isolates and was associated with other virulence markers: pap (1), stb (8), cs31a (3), stb and vt2 (2), cs31a and stb (3), east1 and kps (1), stb and east1 (1), cs31a and east1 (1), and cs31a, stb, pap, and iucD (1). Serogroups were determined for 3 isolates: O93:H4, O83:H19, and O15:H11. Phylogenetic analysis showed that 23 isolates belonged to group A and 4 belonged to B1. The findings revealed that these E. coli isolates are opportunistic pathogens with different virulence factors. The results indicate that the pathogenicity route of E. coli in bovine mastitis is not a consequence of 1 specific virulence factor.

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

“…In the previous studies, researchers also stated variations in the presence rates for these genes [5,20,21,22]. Although, Momtaz et al (2012) and Kobori et al (2004) stated a higher presence rate, other researchers [18,23,24] could not detect the gene in E. coli strains. In the here presented study, stx gene presence was higher than in previous studies, as intimin was lower.…”

“…Researchers stated biofi lm production in E. coli strains in previous reports [18,27,28]. Friedman et al (2013) classifi ed the biofi lm production property of the strains as strong and medium, and found medium level production in one third of the 55 strains.…”

“…The presence of the aerobactin gene was evaluated in previous studies [10,17,18]. Although, Lin et al (1998) defi ned the gene in all strains of E. coli, some researchers [10,18] suggested smaller rates (4-11%) of aerobactin gene presence. Conversely, none of the strains carried the gene in the current study.…”

Sistemas de efluxo multidrogas em Escherichia coli e uso de inibidores como possíveis adjuvantes na terapia da mastite bovina

“…Foram utilizados 27 isolados de Escherichia coli obtidos de leite provenientes de bovinos com mastite (Fernandes et al, 2011 …”

Akut Sığır Mastitislerinden İzole Edilen Escherichia coli Suslarının Filogruplandırılması ve Virulens Faktör Değerlendirmesi

“…The least two phylogroups determined from acute bovine mastitis were found to be D and F. Most of the commensal and intestinal strains have been reported as group A and B1, extra-intestinal E. coli strains belonged to group B2 and D in other studies [6,7] . In the previous studies, bovine mastitis isolates have been generally belonged to A and B1 groups [8,9,[26][27][28] . However, phylogroup C was defined to be closely related to, but distinct from B1 [29,30] .…”